Life Sciences Commons^™

Hippocampal Ca1 Transcriptional Profile Of Sleep Deprivation: Relation To Aging And Stress, Nada M. Porter, Julia H. Bohannon, Meredith Curran-Rauhut, Heather M. Buechel, Amy L.S. Dowling, Lawrence D. Brewer, Jelena Popovic, Veronique Thibault, Susan D. Kraner, Kuey-Chu Chen, Eric M. Blalock

Pharmacology and Nutritional Sciences Faculty Publications

BACKGROUND: Many aging changes seem similar to those elicited by sleep-deprivation and psychosocial stress. Further, sleep architecture changes with age suggest an age-related loss of sleep. Here, we hypothesized that sleep deprivation in young subjects would elicit both stress and aging-like transcriptional responses.

METHODOLOGY/PRINCIPAL FINDINGS: F344 rats were divided into control and sleep deprivation groups. Body weight, adrenal weight, corticosterone level and hippocampal CA1 transcriptional profiles were measured. A second group of animals was exposed to novel environment stress (NES), and their hippocampal transcriptional profiles measured. A third cohort exposed to control or SD was used to validate transcriptional results …

Cdc42-Dependent Activation Of Nadph Oxidase Is Involved In Ethanol-Induced Neuronal Oxidative Stress, Xin Wang, Zunji Ke, Gang Chen, Mei Xu, Kimberly A. Bower, Jacqueline A. Frank, Zhuo Zhang, Xianglin Shi, Jia Luo

Pharmacology and Nutritional Sciences Faculty Publications

It has been suggested that excessive reactive oxygen species (ROS) and oxidative stress play an important role in ethanol-induced damage to both the developing and mature central nervous system (CNS). The mechanisms underlying ethanol-induced neuronal ROS, however, remain unclear. In this study, we investigated the role of NADPH oxidase (NOX) in ethanol-induced ROS generation. We demonstrated that ethanol activated NOX and inhibition of NOX reduced ethanol-promoted ROS generation. Ethanol significantly increased the expression of p47^phox and p67^phox, the essential subunits for NOX activation in cultured neuronal cells and the cerebral cortex of infant mice. Ethanol caused serine …