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Carbohydrate Response Element Binding-Protein Is Activated By Elevated Glucose Levels In The Normal Murine Liver Cell Line Nmuli, Jackson Adams, Daniel G. Peterson
Carbohydrate Response Element Binding-Protein Is Activated By Elevated Glucose Levels In The Normal Murine Liver Cell Line Nmuli, Jackson Adams, Daniel G. Peterson
Daniel G. Peterson
Carbohydrate response element (ChRE) binding protein (ChREBP) is a transcription factor that is regulated by intracellular glucose levels and activates several lipogenic genes including acetyl CoA carboxylase (ACC). Previous studies of the ChREBP system have used both in vivo models as well as isolated primary hepatocytes. Due to the difficulties in isolation and genetic variability of primary cell culture, we propose the use of a normal murine liver cell line (NMuLi) as a convenient alternative to primary isolated hepatocytes. We show that high (25 mM) but not low (0.5 or 5 mM) glucose activates a luciferase reporter driven by 4 …