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Full-Text Articles in Life Sciences

Enzymatic Screening And Genotypic Characterization Of Thermophilic Bacteria From The Hot Springs Of Sarawak, Malaysia, Seng Chiew Toh, Samuel Lihan, Sui Sien Leong, Azizul Hakim Lahuri, Wai Cheong Woon, Wing Woh Ng Dec 2023

Enzymatic Screening And Genotypic Characterization Of Thermophilic Bacteria From The Hot Springs Of Sarawak, Malaysia, Seng Chiew Toh, Samuel Lihan, Sui Sien Leong, Azizul Hakim Lahuri, Wai Cheong Woon, Wing Woh Ng

Makara Journal of Science

Owing to their eccentric thermostable ability, thermophiles are among the most utilized extremophiles in various industries, such as manufacturing, and clinical research. Researchers believe that many unknown thermophiles are yet to be discovered. This study aimed to genotypically characterize the diversity of thermophiles and screen them for the potential production of enzymes in the recreational hot springs located at Northwest Coast of Borneo. Water samples were collected at 45 °C–50 °C from Annah Rais and Panchor hot springs during the sampling period from January 2018 to January 2019. Three samples (water and sediment) were collected twice in a 3-week interval …


Soil Enzyme Activities As Bio Indicators Of Soil Ph And Fertility In Temperate Grassland, Iker Mijangos, A. Ibarra, Isabel Albizu, Sorkunde Mendarte, Carlos Garbisu Aug 2023

Soil Enzyme Activities As Bio Indicators Of Soil Ph And Fertility In Temperate Grassland, Iker Mijangos, A. Ibarra, Isabel Albizu, Sorkunde Mendarte, Carlos Garbisu

IGC Proceedings (1997-2023)

In recent years, biological indicators are being used to estimate the continued capacity of a given soil to function (i.e., soil health). After all, biological processes are intimately linked with the maintenance of soil structure and fertility, being more sensitive to changes in the soil than conventional physicochemical parameters. Soil enzymes, as mediators and catalysts of vital soil functions, offer great potential as integrative indicators of soil health (Dick et al., 1996). The main aim of the current work was to study the potential of soil enzyme activities as biological (more precisely, biochemical) indicators of soil physicochemical …


Understanding The Intracellular Positive Regulatory Interactions In The Metabolic Network, Sultana Mohammed Al Zubaidi Jun 2023

Understanding The Intracellular Positive Regulatory Interactions In The Metabolic Network, Sultana Mohammed Al Zubaidi

Theses

Metabolism is an essential cellular process that produces essential components such as energy molecules and cellular building blocks. It is regulated by intracellular metabolite-enzyme interactions, which can increase or decrease enzyme efficiency. Each metabolic process is not evolved in nature and may not contribute to the optimal efficiency of the organism's metabolism. However, this study aims to clarify the relationship between cellular positive regulatory interactions and the cell's metabolic network, especially how these relationships regulate metabolism and how it evolved in nature. To obtain that, for each enzyme, the activation molecules from the BRENDA database were downloaded, mapped data to …


Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger May 2023

Effect Of Ferulic Acid Esterase Enzyme Application On The In Vitro Digestibility And In Situ Rumen Degradability Of Tropical Grasses, Adegbola T. Adesogan, N. Krueger, C. R. Staples, D. B. Dean, W. Krueger

IGC Proceedings (1997-2023)

Tropical grasses are the primary staple diet of most of the domesticated ruminants in the tropical and subtropical regions of the world. However, dry matter (DM) digestibility (DMD) and intake levels of these C4 grasses are considerably low, partly because of high lignin contents. Ferulic acid also impedes fibre digestion in such forages due to formation of cross linkages with digestible xylans. Certain esterase enzymes have been shown to cleave ferulic acid cross linkages in wheat bran but no studies have examined whether such enzymes can be used to increase the digestion of tropical grasses. Therefore, the objective of …


The Effects Of Exogenous Enzyme Supplementation On The Performance, Bone Quality, And Nutrient Utilization Of Broiler Chickens, Megan Bauer Jan 2023

The Effects Of Exogenous Enzyme Supplementation On The Performance, Bone Quality, And Nutrient Utilization Of Broiler Chickens, Megan Bauer

Theses and Dissertations--Animal and Food Sciences

Two experiments were conducted to examine the effects of Allzyme® Spectrum supplementation to a reduced nutrient diet on the growth performance, energy and nutrient digestibility and utilization, bone quality, and jejunal digesta viscosity in broiler chickens. Each experiment used 300 one-day old Cobb by-product breeder chicks with 5 treatments, 10 replicates, and 6 birds per replicate cage in a randomized complete block design. The only differences between the two experiments were experiment 1 lasted for 21 days using a corn-soybean meal-based diet and experiment 2 lasted for 22 days using a wheat-soybean meal-based diet. The 5 treatments included a positive …