Life Sciences Commons^™

A Glass Bead Semi-Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares, Daniel P. Schachtman, Sophie Alvarez, Karin V. Van Dijk

Department of Agronomy and Horticulture: Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage. Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl2, …

Combining Isotopic And Genetic Analyses To Quantify Microbial Facilitation Of Recalcitrant Resource Use By Terrestrial And Aquatic Consumers, Alexi Christina Besser

Biology ETDs

Quantifying the flow of energy and nutrients through food webs is foundational to understanding the structure and function of ecosystems. Here, I utilize the stable carbon and nitrogen isotope analysis of individual amino acids to trace the movement of essential amino acids through terrestrial and freshwater food webs in New Mexico, USA. I first explore isotopic patterns among co-occurring terrestrial plants and aquatic algae. I then combine this molecular isotopic approach with 16S and 18S rRNA sequencing to demonstrate the importance of gut microbiota as sources of essential amino acids to wild mammalian hosts. Next, I explore the roles of …

Study On Antioxidant Activities Of Proteins And Enzymatic Hydrolysates Of Eight Edible Fungi, Wang Yao-Ran, Zhao Yan, Chen Ming-Jie, Wei Chen-Ying, Zha Lei, Li Zhi-Ping

Food and Machinery

Objective: This study focused on the antioxidant activities of eight edible fungiproteins and their hydrolysates. Methods: Eight edible fungi proteins were hydrolyzed using alkaline protease. DPPH free radical scavenging activity, ABTS free radical scavenging activity, Fe²⁺ chelation rate and reducing power were used to evaluate antioxidant activity. Results: SDS-PAGE electrophoretic profile results indicated that alkaline protease was efficient in hydrolyzing proteins into low molecular weight peptides and amino acid; the antioxidant activity of the alkaline proteolysis product of Volvariellavolvacea was the highest with DPPH free radical scavenging ability (5 140.45±5.35) μg Trolox/g, ABTS free radical scavenging …

Determination Of Sugars And Amino Acids In Japanese Wine Using Core–Shell Liquid Chromatography Tandem Electrochemical Detection, Kana Yoshimura, Aiko Sano, Ryuichiro Suzuki, Shun-Ichi Mitomo, Yukiko Negishi, Toshiki Mutai, Florencio Arce Jr, Gerard Lee See, Takashi Tanikawa, Yutaka Inoue

The Thai Journal of Pharmaceutical Sciences

In this study, sugars (glucose, fructose, and sucrose) and amino acids in plum and yuzu wines were quantified with a new and simple method involving an electrochemical detector (ECD) and core–shell column (S-30/70 = Styrene/divinylbenzene – 5 tetramethyldiaminohexane). Analysis was conducted under the following conditions: A column temperature (40°C), flow rate (0.3 mL/min), injection volume (20 μL), and mobile phase (0.1 mol/L NaOH). Glucose, fructose, and sucrose had retention times of 14.68 min, 16.22 min, and 19.29 min, respectively, when measured using an ECD. When the sugar content was compared among the plum wines, plum wine A had the lowest …

Study On Microwave Heating On Nutrition And Flavor Composition Of Crayfish, Xu Wen-Si, Yang Qi-Fu, Zhao Zi-Long, Yan Ming-Zhu, Wu Shuang-Qing, Wang Yan, Yang Pin-Hong

Food and Machinery

Objective:The effect of microwave heating on nutrition and flavor composition of crayfish was investigated.Methods:After pre-treatment, the nutrition and flavor changes of crayfish without microwave treatment, 210 W microwave and 350 W microwave treatment for 5 min were analyzed respectively.Results:The results showed that low power microwave treatment did not significant affect the crayfish water content, which was obvious loss under the condition of 350 W microwave. In dry samples, the microwave power had no significant effect in crude ash, while the total sugar and crude fat was affected with a decrease tendency. However, the microwave power …

Amino Acids As Alternatives To Emulsifying Salts In Processed Cheese Analogues, Jeremy Thomas Pack

Theses and Dissertations

Background: Manufacturers have used emulsifying salts universally in the processed cheese industry since James L. Kraft patented the first processed cheese in 1916. My objective was to find alternative ingredients to replace emulsifying salts in processed cheese formulations; the product would follow "clean-labeling" trends and lower the final formulation's sodium content. Materials and Methods: My experiments followed conventional processed cheese formulations to create experimental batches, which were compared to positive and negative controls for significance. Textural, rheological, and melting tests evaluated objective cheese parameters. We observed subjective sensory properties through qualitative descriptive analysis, consumer acceptance panels, and focus groups. Results: …

Essential Amino Acid (Eaa) Regulation Of Skeletal Muscle Protein Turnover With Age, Mary Komp

Graduate Theses and Dissertations

Skeletal muscle (SM) is vital for both long term health and quality of life. Recent research suggests an increase in catabolic signals with age triggers pathologic conditions, such as sarcopenia. Although results from in vitro studies model how EAA can regulate muscle protein synthesis (MPS), the relevance of these models to muscle protein breakdown (MPB) and the presence of physiological EAA concentrations remains to be established. Therefore, the objective of this study was to determine the effects of a low, normal, and supra physiological dose of EAA (0.2, 1.0, and 3.0 x EAA) in a young (passages 2-10) and aging …

A Glass Bead Semi‑Hydroponic System For Intact Maize Root Exudate Analysis And Phenotyping, Martha G. Lopez-Guerrero, Peng Wang, Felicia Phares4, Daniel P. Schachtman, Sophie Alvarez, Karin Van Dijk

Department of Biochemistry: Faculty Publications

Background: Although there have been numerous studies describing plant growth systems for root exudate collection, a common limitation is that these systems require disruption of the plant root system to facilitate exudate collection. Here, we present a newly designed semi-hydroponic system that uses glass beads as solid support to simulate soil impedance, which combined with drip irrigation, facilitates growth of healthy maize plants, collection and analysis of root exudates, and phenotyping of the roots with minimal growth disturbance or root damage.

Results: This system was used to collect root exudates from seven maize genotypes using water or 1 mM CaCl …

High Protein Yeast-Based Ddgs As An Alternative To Commonly Used Protein Sources In Pig Diets, Yesid Ricardo Garavito Duarte

Electronic Theses and Dissertations

Alternative ingredients and co-products from ethanol and biofuel industries represent an opportunity for utilization in swine diets. In this context, understanding nutritional value to practical swine feeding begins with product characterization and determination of nutrient digestibility. The objective of this research was to test the hypothesis: yeast-based high-protein DDGS (HP-GDDY) has comparable AA and metabolizable energy (ME) digestibility to feedstuffs commonly used in swine diets and that it could be included as an alternative protein source in nursery diets without negative impact on growth performance. Cornstarch-based diets were formulated using six ingredients as the sole source of AA: spray dried …

Determining The Impact Of Corn Source On Nutrient Availability And Pig Performance, And Improving Boar Performance During Heat Stress, Jordan Jansen

Electronic Theses and Dissertations

The quality and effectiveness of the feedstuffs included in diets of production livestock directly impact yield efficiency and performance characteristics desired by producers. This thesis focuses on the performance and digestibility of corn from different countries in pig diets and how different feed additives can be supplemented to improve boar reproductive performance during heat stress. Corn is the largest energy source in common pig diets. The physical characteristics of corn such as broken kernels, foreign material, and excessive dust are said to negatively impact the quality of the grain for pig diets by being less digestible and a worsened nutrient …

A Study Protocol To Prepare An Rbd Protein For Vaccine Against Covid-19, Z.M.G. Sarwar Jahangir, Arleta Helena Marnik

Publications and Research

Background: SARS-CoV-2 pandemic is a global threat to humans and the world’s economy. Effective and safe vaccines against this virus are essential to control and eradicate the pandemic. The currently applied vaccines carry SARS-CoV-2 spike-protein mRNA/cDNA. These vaccines go through several cellular processes in the recipients for producing antigens. On the contrary, the SARS-CoV-2 RBD (receptor binding domain)-protein is an antigen. It will directly stimulate antibody production against SARS-CoV-2. Hence, we propose to produce SARS-CoV-2 RBD-protein as a fast acting, effective and safe vaccine.

Methods: We propose to reconstruct a plasmid carrying three types of DNA sequences: RBD cDNA, FP …

Determination Of Sugars And Amino Acids In Japanese Wine Using Core-Shell Liquid Chromatography Tandem Electrochemical Detection, Kana Yoshimura, Aiko Sano, Ryuichiro Suzuki, Shun-Ichi Mitomo, Yukiko Negishi, Toshiki Mutai, Florencio Arce Jr., Gerard Lee See, Takashi Tanikawa

The Thai Journal of Pharmaceutical Sciences

In this study, sugars (glucose, fructose, and sucrose) and amino acids in plum and yuzu wines were quantified with a new and simple method involving an electrochemical detector (ECD) and core-shell column [S-30/70=St (styrene)/DVB (divinylbenzene)-5TMDAH (tetramethyldiaminohexane)]. Analysis was conducted under the following conditions: A column temperature (40°C), flow rate (0.3 mL/min), injection volume (20 μL), and mobile phase (0.1 mol/L NaOH). Glucose, fructose, and sucrose had retention times of 14.68 min, 16.22 min, and 19.29 min, respectively, when measured using an ECD. When the sugar content was compared among the plum wines, plum wine A had the lowest sugar content. …