Life Sciences Commons^™

Chronic Inflammation Was A Major Predictor And Determinant Factor Of Anemia In Lactating Women In Sidama Zone Southern Ethiopia: A Cross-Sectional Study, Tafere Gebreegziabher Belay, Taylor Roice, Barbara J. Stoecker

All Faculty Scholarship for the College of the Sciences

Anemia in women of reproductive age is highly prevalent globally and remains a public health problem. In Ethiopia, despite efforts to minimize the burden of anemia, it is still a moderate public health problem. Anemia has various etiologies including nutritional deficiency, parasitic infection, and inflammation. The aim of this study was to examine contributing factors to anemia in lactating women. Following ethical approval, and six months after delivery, all lactating women (n = 150) were recruited to participate in this study from eight randomly selected rural villages. Anthropometric and socio-economic factors were assessed. From each, a blood sample was collected …

Hot But Not Dry: Modest Changes In Water Relations For An Epiphytic Bromeliad In A Tropical Dry Deciduous Forest, Saúl Hernández-Robinson, Eric A. Graham, Olivia Hernández-González, Roberth Us-Santamaría, José Luis Simá, Fernando Arellano-Martín, José Luis Andrade

All Faculty Scholarship for the College of the Sciences

Premise of research. Epiphytic bromeliads endure intense seasonal environmental changes in the canopy of dry tropical deciduous forests. The analysis of the physiological responses of these epiphytes to environmental changes can be useful in assessing their plasticity, vulnerability, and adaptations to such extreme habitats.

Methodology. We measured microenvironmental variables and water relations for plants of the epiphytic bromeliad Tillandsia brachycaulos in three microhabitats within the canopy of a dry tropical forest. We measured individual plants for seasonal and spatial differences in light, leaf temperature, osmotic potential, cell wall elasticity, and relative capacitance as indications of their physiological responses …

Post-Exercise Sweat Loss Estimation Accuracy Of Athletes And Physically Active Adults: A Review, Eric O'Neal, Tara Boy, Brett Davis, Kelly Pritchett, Robert Pritchett, Svetlana Nepocatych, Katherine Black

All Faculty Scholarship for the College of Education and Professional Studies

The main purposes of this review were to provide a qualitative description of nine investigations in which sweat losses were estimated by participants following exercise and to perform a quantitative analysis of the collective data. Unique estimations (n = 297) were made by 127 men and 116 women after a variety of exercise modalities in moderate to hot environmental conditions. Actual sweat loss exceeded estimated sweat loss (p < 0.001) for women (1.072 ± 0.473 vs. 0.481 ± 0.372 L), men (1.778 ± 0.907 vs. 0.908 ± 0.666 L) and when all data were combined (1.428 ± 0.806 vs. 0.697 ± 0.581 L), respectively. However, estimation accuracy did not differ between women (55.2 ± 51.5%) and men (62.4 ± 54.5%). Underestimation of 50% or more of sweat losses were exhibited in 168 (54%) of estimation scenarios with heavier sweaters displaying a higher prevalence and trend of greater underestimations in general. Most modern guidelines for fluid intake during and between training bouts are based on approximate sweat loss estimation knowledge. These guidelines will likely have minimal efficacy if greater awareness of how to determine sweat losses and accurate recognition of sweat losses is not increased by coaches and athletes.

Infant Attraction: Why Social Bridging Matters For Female Leadership In Tibetan Macaques, Xi Wang, Dong-Po Xia, Lixing Sun, Paul A. Garber, Randall C. Kyes, Lori K. Sheeran, Bing-Hua Sun, Bo-Wen Li, Jin-Hua Li

Biology Faculty Scholarship

Leadership is a key issue in the study of collective behavior in social animals. Affiliation–leadership models predict that dyadic partner preferences based on grooming relationships or alliance formation positively affect an individual’s decision to follow or support a conspecific. In the case of many primate species, females without young infants are attracted to mother–infant dyads. However, the effects of mother–infant–female associations on affiliation–leadership models remain less clear. In free-ranging Tibetan macaques Macaca thibetana, we used social network analysis to examine the importance of “mother-infant-adult female” social bridging events as a predictor of who leads and who follows during group …

No Significant Differences In Muscle Growth And Strength Development When Consuming Soy And Whey Protein Supplements Matched For Leucine Following A 12 Week Resistance Training Program In Men And Women: A Randomized Trial, Heidi M. Lynch, Matthew P. Buman, Jared M. Dickinson, Lynda B. Ransdell, Carol S. Johnson, Christopher M. Wharton

All Faculty Scholarship for the College of the Sciences

There are conflicting reports regarding the efficacy of plant versus animal-derived protein to support muscle and strength development with resistance training. The purpose of this study was to determine whether soy and whey protein supplements matched for leucine would comparably support strength increases and muscle growth following 12 weeks of resistance training. Sixty-one untrained young men (n = 19) and women (n = 42) (18–35 year) enrolled in this study, and 48 completed the trial (17 men, 31 women). All participants engaged in supervised resistance training 3×/week and consumed 19 grams of whey protein isolate or 26 grams of soy …

Catalytic, Selective, And Stereocontrolled Construction Of C4 Quaternary And Homobenzylic Dihydroisoquinolones By Sp3 C–H Benzylation, Timothy K. Beng, Antonio Moreno

All Faculty Scholarship for the College of the Sciences

C1 benzylated isoquinoline derivatives constitute the core of benzylisoquinoline alkaloids (BIAs). However, their C4 congeners remain elusive. Here, we describe a diastereoselective, catalytic, and modular C(sp³)–C(sp³) coupling protocol wherein β-amino sp³ C–H bonds of readily affordable vicinally functionalized dihydroisoquinolones are replaced by sp³ C–benzyl bonds. The method provides expedient access to C4 quaternary and homobenzylic dihydroisoquinolones, which are attractive fragments for potential drug discovery.

Detection Of Neopterin In The Urine Of Captive And Wild Platyrrhines, Alexandra J. Sacco, Jessica A. Mayhew, Mrinalini Watsa, Gideon Erkenswick, April K. Binder

All Faculty Scholarship for the College of the Sciences

Background: Non-invasive biomarkers can facilitate health assessments in wild primate populations by reducing the need for direct access to animals. Neopterin is a biomarker that is a product of the cell-mediated immune response, with high levels being indicative of poor survival expectations in some cases. The measurement of urinary neopterin concentration (UNC) has been validated as a method for monitoring cell-mediated immune system activation in multiple catarrhine species, but to date there is no study testing its utility in the urine of platyrrhine species. In this study, we collected urine samples across three platyrrhine families including small captive populations of …

The Nutritional Status Of Children Living Within Institutionalized Care: A Systematic Review, Emily Delacey, Cally Tann, Nora Groce, Maria Kett, Michael Quiring, Ethan Bergman, Caryl Garcia, Marko Kerac

All Faculty Scholarship for the College of the Sciences

Background. There are an estimated 2.7 million children living within institutionalized care worldwide. This review aimed to evaluate currently available data on the nutrition status of children living within institutionalized care.

Methods. We searched four databases (Pubmed/Medline, CINHAL Plus, Embase and Global Health Database) for relevant articles published from January 1990 to January 2019. Studies that included information on anthropometry or micronutrient status of children living within institutionalized care were eligible for inclusion. The review is registered on PROSPERO: CRD42019117103.

Results. From 3,602 titles screened, we reviewed 98 full texts, of which 25 papers were eligible. Two (8%) studies reported …

The Behavioral Ecology Of The Tibetan Macaque, Jin-Hua Li, Peter M. Kappeler, Lixing Sun

All Faculty Scholarship for the College of the Sciences

This open access book summarizes the multi-disciplinary results of one of China’s main primatological research projects on the endemic Tibetan macaque (Macaca thibetana), which had continued for over 30 years, but which had never been reported on systematically. Dedicated to this exceptional Old World monkey, this book makes the work of Chinese primatologists on the social behavior, cooperation, culture, cognition, group dynamics, and emerging technologies in primate research accessible to the international scientific community.

Using Cuda To Enhance Data Processing Of Variant Call Format Files For Statistical Genetic Analysis, Heather Mckinnon

All Graduate Projects

Utilizing the power of GPU parallel processing with CUDA can speed up the processing of Variant Call Format (VCF) files and statistical analysis of genomic data. A software package designed toward this purpose would be beneficial to genetic researchers by saving them time which they could spend on other aspects of their research. A data set containing genetics from a study of trichome production in Mimulus guttatus, or yellow monkey flower, was used to develop a package to test the effectiveness of GPU parallel processing versus serial executions. After a serial version of the code was generated and benchmarked, OpenACC …

Ticks On Lizards: Parasite-Host Interactions Of The Southern Alligator Lizard (Elgaria Multicarinata) In Washington State, Emma Houghton

All Undergraduate Projects

I investigated interactions between ectoparasites (ticks) and their host, the southern alligator lizard (Elgaria multicarinata). Alligator lizards are capable of complement-mediated killing of the Lyme disease spirochete carried by ticks and may potentially reduce Lyme disease prevalence by cleansing pathogenic organisms from ticks. Despite this, little is known about host-parasite dynamics in alligator lizards. My goals were to 1) assess patterns of tick presence (i.e. parasite load) on alligator lizards and 2) investigate potential negative effects of ticks on alligator lizards. I sampled lizards during the summer of 2019 near Catherine Creek, along the Columbia River Gorge in southern Washington. …

Toadal Isolation: Genetic Connectivity Of The Western Toad (Anaxyrus Boreas) Along I-90 In The Snoqualmie Pass Area Of Washington State, Anneliese Myers

All Master's Theses

Single-nucleotide polymorphisms (SNPs) were used to assess the genetic connectivity of western toad (Anaxyrus boreas) breeding populations along Interstate-90 near Snoqualmie Pass, WA. Sites north and south of the freeway were sampled during the breeding season of 2019. SNP loci were subsequently generated using the proprietary DArTseq^TM (Canberra, ACT, Australia) method. A total of 15,468 SNPs were used to calculate pairwise F_ST values and three distinct breeding populations were identified, two north and one south of I-90. All pairwise F_ST values between these sites were low (0.02) but significantly different from 0. The lowest pairwise …

Effects Of Western Spruce Budworm Herbivory On Forest Soils And Litter Decomposition In Central Washington, Izak R. Neziri

All Master's Theses

Disturbances by herbivores can drive nutrient cycling in forest ecosystems by adding frass, carcasses, and molts to the forest floor which are broken down into nitrogen, phosphorous, and other elements to be recycled into biomass. Western spruce budworms are defoliators native to the central Cascades and their herbivory could increase the decomposition rate of forest materials by adding essential nutrients and/or by increasing light and rainfall penetration to the forest floor by thinning the forest canopy during outbreaks. Budworm defoliation events are expected to increase in severity as the climate warms, potentially altering forest ecosystem function. The purpose of this …

Floral Trait Architecture In Crop Sunflower (Helianthus Annuus) Under Drought Conditions, Lauren Segarra

All Master's Theses

Longer and more intense droughts are predicted to become more common in the coming century due to anthropogenic climate change. Drought can reduce crop yield and decrease food security. In order to mitigate the negative effects of drought on crop production, it is important to elucidate the underlying mechanisms that promote drought stress resistance in crop plants. Floral traits impact yield, especially in oilseed crops such as sunflower (Helianthus annuus), but their susceptibility to drought stress is understudied. The goal of this study was to describe the floral trait architecture of H. annuus crop lines under drought versus …

Disparities In The Prevalence And Risk Factors Of Anaemia Among Children Aged 6–24 Months And 25–59 Months In Ethiopia, Tafere Gebreegziabher Belay, Nigatu Regassa, Micaela Wakefield, Kelly Pritchett, Susan Hawk

All Faculty Scholarship for the College of Education and Professional Studies

Despite global efforts made to address anaemia, the prevalence remains high in most Sub-Saharan African countries. In Ethiopia, anaemia poses a very strong public health concern. The purpose of the present study was to examine the key risk factors related to anaemia among children aged 6–24 months (younger age group) and 25–59 months (older age group). We used the 2016 Ethiopian Demographic and Health Survey data, collected from 11 023 mothers with under five children. Ordered logistic regression modelling was used for assessing risk factors of childhood anaemia. The results suggest that the prevalence of anaemia was 72 % in …

The Effects Of Induced Polycystic Ovary Syndrome In Nag-1 Transgenic Mice, Nicholas Werner

All Master's Theses

Polycystic ovary syndrome (PCOS) is the leading cause of infertility among women in the US and the most common endocrine disorder among women. PCOS is characterized by cystic ovaries, hyperandrogenism (heightened levels of male sex hormones), altered menstrual cycles and various metabolic dysfunctions. The metabolic symptoms associated with PCOS are difficult to treat, as they are a result of hormonal imbalances, rather than diet. The human Non-Steroidal Anti-Inflammatory Drug Activated Gene (NAG-1) been shown to prevent diet-induced metabolic disorders and weight gain in mice. We hypothesized that the expression of NAG-1 may also prevent hormonal-induced metabolic disorders. To test this …

Diversity Analysis Of Soil Fungus Communities In Disturbed, Nursery, And Mature Forest Conditions, Dana Whitmore

All Master's Theses

Populations of soil fungi were examined in Derby Canyon Natives, Coeur d’Alene Forest Service nursery, Swamp Lake, and the Keechelus Lake wildlife overcrossing soils. All sampling sites were connected by their relation to the revegetation and native soil plug inoculation of the wildlife overpass. This study was an effort to describe soil fungi communities present on the overpass before plant introduction, those that plants would be bringing in their pots, and the fungi that could be introduced via soil plug transplantation. DNA was extracted from soil samples, then sequenced using next-generation sequencing methods, allowing for the analysis of species richness …

Evaluation Of The Relationship Between Land Use And Water Quality In Kittitas County, Wa, Lindsay Schulz

All Master's Theses

Water in Kittitas County is extremely valuable since it supports farming, recreation, and cultural activities, as well as environmental processes and a diversity of biological life while providing many ecosystem services. However, land conversions required by agricultural and urban land uses can negatively impact water quality and the biological function of the stream. I studied how forested, agricultural, and urban land use affect six streams. Fourteen sites were sampled, once each in July, August, and September 2019. Land use was calculated as a percentage of forested, agricultural, and urban land use within a 100-m buffer of the stream, upstream of …

Risk Of Low Energy Availability In Elite Male Athletes With Spinal Cord Injury, Savannah Glasgow

All Master's Theses

Recent research examining male able-bodied athletes has suggested that males are at risk for low energy availability (LEA); however, there is a paucity of research pertaining to prevalence amongst male athletes with an impairment. Therefore, the purpose of this study was to examine the risk of LEA and prevalence of symptoms related to Relative Energy Deficiency in Sport (RED-S), including risk of disordered eating, hormonal profiles, and bone mineral density (BMD) scans, in male wheelchair para athletes. Collegiate- and national-level male athletes (n = 9) with spinal cord injury (SCI) completed 7-day concurrent diet and training logs, Eating Disorder Examination …

Sampling The Local Fare: Fishes At The Sam Israel House Pit (45gr76), Soap Lake, Washington, Adam Fruge

All Master's Theses

The Sam Israel site is a precontact archaeological complex with numerous fish bones at the north end of Soap Lake, Washington. Excavated in 1976, the fish remains recovered from there were never fully analyzed prior to this research. Since this inland Columbia Plateau site had thousands of fish bones, it contained untapped potential for our understanding of ancient local fish procurement. As such, I conducted a detailed analysis of 2,862 fish bone specimens from the Sam Israel House Pit locus to: study a larger sample of fish bones in greater detail than was done before; compare the distribution of fishes …

Environmental Dna Is An Effective Method To Monitor Species In Various Freshwater Habitats, Kayleigh Mullen

All Master's Theses

This research investigated the use of DNA shed from individuals into the environment (eDNA) to monitor three amphibian species and two trout species associated with habitat intersected by Interstate-90 in Snoqualmie Pass, Washington. This included a large catchment area within creeks and nearby wetlands historically affected by I-90, including sites where significant habitat improvements had been made. Species-specific primers were used to detect three focal amphibians of varying local abundance and two focal trout species. This study showed successful detection of species across both lentic and lotic systems throughout the study area through efficient multiplexing (detection of multiple species in …

Early Life History And Stock Discrimination Of Kokanee Salmon (Oncorhynchus Nerka) In An Alpine Lake Environment, Alexandra Mccarrel

All Master's Theses

This study examines an ecologically and recreationally important population of kokanee salmon (Oncorhynchus nerka) residing in Keechelus Lake and its tributary Gold Creek in the central Cascades of Washington State. This population of kokanee salmon is a vital food base for a population of ESA-listed resident bull trout. However, little is known about the early life history of this population and how it interacts with unique features in its rearing environment. With my research I described the early life history of kokanee salmon that spawn in the lake’s main tributary, Gold Creek, and proposed a framework to determine …

Ecological And Genetic Connectivity Of Shrews (Sorex Spp.) Across Interstate-90 In The Washington Cascade Range, Jordan Ryckman

All Master's Theses

Roads negatively affect wildlife by degrading and fragmenting habitat. The I-90 Snoqualmie Pass East project was established to improve traffic safety for both humans and wildlife. Here, the Washington State Department of Transportation is constructing wildlife crossing structures to increase ecosystem connectivity as part of this project. The goal of my study was to investigate shrews – very small mammals with presumably low mobility and dispersal capacity – at sites on the eastern slopes of the Cascade Range where wildlife crossing structures will be built in the future. My main objectives were to verify shrew species composition, assess population genetic …

Ticks On Lizards: Parasite-Host Interactions Of The Southern Alligator Lizard (Elgaria Multicarinata) In Washington State, Emma Houghton

Undergraduate Honors Theses

I investigated interactions between ectoparasites (ticks) and their host, the southern alligator lizard (Elgaria multicarinata). Alligator lizards are capable of complement-mediated killing of the Lyme disease spirochete carried by ticks and may potentially reduce Lyme disease prevalence by cleansing pathogenic organisms from ticks. Despite this, little is known about host-parasite dynamics in alligator lizards. My goals were to 1) assess patterns of tick presence (i.e. parasite load) on alligator lizards and 2) investigate potential negative effects of ticks on alligator lizards. I sampled lizards during the summer of 2019 near Catherine Creek, along the Columbia River Gorge in southern Washington. …

An Analytical Examination On The Effects Of Vegetarian And Omnivorous Diets On C-Reactive Protein, Aletha Kleis

Undergraduate Honors Theses

There is a lack of research regarding how following a vegetarian or omnivores diet effects C-Reactive Protein (CRP) levels of people as seen through results from an analysis of data gathered from the National Health and Nutrition Examination Survey (NHANES). The level of CRP is a reflection of how much inflammation there is in one’s body and is a popular indicator of risk for heart disease. Thus, in this research, I use the NHANES data to look at the relationship of CRP levels of people who identified themselves as vegetarian or not, while also considering the general healthiness of each …

Investigating The Avian Species Composition, Diversity, And Abundance Of Engelhorn Pond, Kiana Rose

Undergraduate Honors Theses

Central Washington University’s Engelhorn Pond contains important habitats for many organisms, particularly migratory birds, and is a valuable resource for students. However, little information is available about the wildlife inhabiting the pond. The pond’s discrete location means that many people are unaware of its existence. In addition, the pond is threatened by a number of anthropogenic sources, including chemical runoff, garbage, and feral cats. By using point counts, a common ornithological survey technique, I recorded the bird species present at the pond during all seasons of the year. I collected data using point counts during 13 visits from June of …

Group Differences In Mother-Infant Macaca Fascicularis Behavior, Parasite Load, And Body Condition Within An Anthropogenically Altered Forest, Elizabeth M.C. Coggeshall

All Master's Theses

This study aimed to establish preliminary health and behavioral data, as well as understand group variation for a large population of Macaca fascicularis individuals within an anthropogenically altered monkey forest. A parasitic analysis of 40 mother and infant individuals showed that M. fascicularis carried 13 different parasitic taxa, and that there was parasitic variation between groups. Body condition scores were determined using a newly created and adapted body condition scale from 146 sampled mother macaques. Body condition scores were significantly different between groups, specifically the pond group when compared to the three other groups. Mother-infant behavioral differences were seen between …

Examining Environmental Use By Captive Lemur Catta And Varecia Rubra, Rhiannon Belcher

All Master's Theses

There are over 100 named species of lemurs, of which 94% are considered threatened with extinction by the International Union for the Conservation of Nature (IUCN). They live in increasingly fragmented forests. To understand how best to protect them in their natural habitats, we can observe how they manipulate the environment and how they use objects, both natural and humanmade, around them. Understanding their behavior is a critical component of conservation, and observing behavior in a captive setting allows us to study lemur-environment relationships without disrupting what little habitat is left in Madagascar. In this study I investigated whether the …

Acoustic Analysis Of Nomascus Gibbon Songs As A Potential Measure Of Current Health Status, Caroline Rowley

All Master's Theses

Post-release monitoring is vital to the rehabilitation process. Gibbons offer a unique challenge, as they are notoriously difficult to follow through the forest, but their duets offer a non-invasive window into their lives. The aim of this project is to create a method that will evaluate the health of rehabilitated and released gibbons through acoustic analyses. Gibbon duets are in part genetically determined, but learning may play a role in song development. Additionally, songs may indicate resource holding potential, and are vital to acquiring mates and producing offspring. Captive-raised gibbons are often denied the experience of co-singing with their parents, …

Mommy Issues: Do Differential Rearing Histories Affect The Social Behavior Of Captive Chacma Baboons (Papio Ursinus), Madeleine Spencer

All Master's Theses

I observed the behavior of 17 chacma baboons (Papio ursinus) in order to understand the relationship between rearing history, dominance hierarchy, and social behavior. The Centre for Animal Rehabilitation and Education (C.A.R.E.) has utilized hand-rearing in the past and is currently using a surrogate-rearing method. Hand-rearing involves one or more primary caregivers attending to an infant for 6 – 12 months. Surrogate-rearing involves one individual being the primary surrogate for the first 3 months of the infant’s life prior to bonding her or him to an adult female baboon before integrating both back into the surrogate’s troop. Mother-reared …