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Full-Text Articles in Life Sciences
Production Of Dynein And Kinesin Motor Ensembles On Dna Origami Nanostructures For Single Molecule Observation, Jingjie Hu, Nathan Derr
Production Of Dynein And Kinesin Motor Ensembles On Dna Origami Nanostructures For Single Molecule Observation, Jingjie Hu, Nathan Derr
Biological Sciences: Faculty Publications
Summary
The goal of this protocol is to form ensembles of molecular motors on DNA origami nanostructures and observe the ensemble motility using total internal reflection fluorescence microscopy.
Abstract
Cytoskeletal motors are responsible for a wide variety of functions in eukaryotic cells, including mitosis, cargo transport, cellular motility, and others. Many of these functions require motors to operate in ensembles. Despite a wealth of knowledge about the mechanisms of individual cytoskeletal motors, comparatively less is known about the mechanisms and emergent behaviors of motor ensembles, examples of which include changes to ensemble processivity and velocity with changing motor number, location, …