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Regeneration Data: Rna Sequences, Abijeet Singh Mehta, Amit Singh
Regeneration Data: Rna Sequences, Abijeet Singh Mehta, Amit Singh
Regeneration data
Abstract for paper for which this research was collected: Notophthalmus viridescens (Red-spotted Newt) possess amazing capabilities to regenerate their organs and other tissues. Previously, using a de novo assembly of the newt transcriptome combined with proteomic validation, we identified a novel family of 5 protein members expressed in adult tissues during regeneration in Notophthalmus viridescens. Due to absence of transgenic approaches in the Notophthalmus, and conservation of genetic machinery across species, we generated transgenic Drosophila melanogaster to misexpress these genes.
Samples for RNA sequencing were collected at third instar larval (L3) developmental stage. Out of the total 36,099 …