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Full-Text Articles in Life Sciences

Pteridium Nuclear Gene Phylogeny, Paul G. Wolf, Carol Rowe, Sylvia Kinosian, Joshua P. Der, Peter J. Lockhart, Lara D. Shepherd, Patricia A. Mclenachan, John A. Thomson Feb 2019

Pteridium Nuclear Gene Phylogeny, Paul G. Wolf, Carol Rowe, Sylvia Kinosian, Joshua P. Der, Peter J. Lockhart, Lara D. Shepherd, Patricia A. Mclenachan, John A. Thomson

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Raw amplicon sequence data and raw reads of from GBS library. Also all code and analyses required to process raw data.


Refernment: An R Package For Annotating Rna Editing In Plastid Genomes, Tanner A. Robison, Paul G. Wolf Jan 2019

Refernment: An R Package For Annotating Rna Editing In Plastid Genomes, Tanner A. Robison, Paul G. Wolf

Biology Faculty Publications

PREMISE OF THE STUDY: In the absence of cDNA, the annotation of RNA editing in plastomes must be done manually, representing a significant time cost to those studying the organellar genomes of ferns and hornworts.

METHODS AND RESULTS: We developed an R package to automatically annotate apparent nonsense mutations in plastid genomes. The software successfully annotates such sites and results in no false positives for data with no sequencing or assembly errors.

CONCLUSIONS: Compared to manual annotation, ReFernment offers greater speed and accuracy for annotating RNA editing sites. This software should be especially useful for researchers generating large numbers of …