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An Approach To Identify Mycobacteriophage Diversity Prior To Dna Sequencing, Charles Gregory
An Approach To Identify Mycobacteriophage Diversity Prior To Dna Sequencing, Charles Gregory
Mahurin Honors College Capstone Experience/Thesis Projects
Over 6,869 Mycobacteriophages have been isolated and purified. Of these, 1,367 genomes have been sequenced at the DNA level and more are added each year through the SEA-PHAGES program. Sequenced mycobacteriophages are grouped into clusters based on a 50% or greater nucleotide identity. The number and breadth of these clusters represents the diversity present in the environment. Each year, as new phages are discovered by students in the SEA-PHAGES program, the question arises, “Which isolates should we sequence?” In order to sequence phages that represent the greatest possible diversity, and thus broaden under-represented clusters and identify new singletons, we need …