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Differential Precipitation And Solubilisation Of Proteins, Barry J. Ryan, Gemma K. Kinsella
Differential Precipitation And Solubilisation Of Proteins, Barry J. Ryan, Gemma K. Kinsella
Books/Book Chapters/ Proceedings
Differential protein precipitation is a rapid and economical step in protein purification and is based on exploiting the inherent physicochemical properties of the polypeptide. Precipitation of recombinant proteins, lysed from the host cell, is commonly used to concentrate the protein of choice before further polishing steps with more selective purification columns (e.g., His-Tag, Size Exclusion, etc.). Recombinant proteins can also precipitate naturally as inclusion bodies due to various influences during overexpression in the host cell. Although this phenomenon permits easier initial separation from native proteins, these inclusion bodies must carefully be differentially solubilized so as to reform functional, correctly folded …