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Prevalence Of Batrachochytrium Dendrobatidis (Bd) In Watercourses Situated In Southeast Tennessee, Macall A. Nabors
Prevalence Of Batrachochytrium Dendrobatidis (Bd) In Watercourses Situated In Southeast Tennessee, Macall A. Nabors
Honors Theses
Four hundred and twenty-seven amphibian species are critically endangered. The fungal disease Batrachochytrium dendrobatidis (Bd) has been correlated with amphibian decline and extinction. Numerous studies concerning the nature of Bd have been conducted; however, additional studies are needed to adequately evaluate Bd in southeastern Tennessee. As a result of previous studies, the following hypothesizes were proposed: watercourses in southeastern Tennessee will test positive for Bd, non-impacted streams will have a greater presence of Bd than impacted streams, and increased canopy coverage has a positive correlation with the presence of Bd. Field sampling took place from June 2nd 2016 to August …
Measuring The Presence Of The Amphibian Pathogen Batrachochytrium Dendrobatidis In East Tennessee, Erin A. Schrenker
Measuring The Presence Of The Amphibian Pathogen Batrachochytrium Dendrobatidis In East Tennessee, Erin A. Schrenker
Honors Theses
I examined the presence and absence of the amphibian pathogen Batrachochytrium dendrobatidis in three survey areas in Eastern Tennessee. Amphibians were swabbed to assess for Bd and other ecological qualities like canopy cover, and air and water temperature are a few of the measurements evaluated. DNA was extracted from the swabs using a Qiagen DNeasy Blood and Tissue Kit and samples were PCRed in triplicate to confirm presence or absence. All 48 animals that were swabbed in the field were negative for Bd. These results parallel other findings that few Bd positive assays have been conducted in East Tennessee from …