Life Sciences Commons^™

Morphological, Genetic, And Environmental Characterization Of An Unusual Population Of Isoetes (Isoetaceae, Lycopodiophyta), Shannon L. Walker

Honors Theses

A large and unusual population of Isoetes (Isoetaceae, Lycopodiophyta) in the DeSoto National Forest, Wayne County, Mississippi, was studied to determine if the individuals there represent a new species or if they represent part of the variation of the one primary species of the longleaf pine belt of Mississippi, Isoetes louisianensis, which it most closely resembles. The unusual population and specimens of known Isoetes louisianensis were examined comparatively based on morphology, megaspore ornamentation, examination of habitat characteristics, and phylogenetic analysis of DNA sequence data from the nuclear internal transcribed spacer 1 and 2 (ITS) and the 5.8S ribosomal gene. No …

An Examination Of The Influence Of Social Physique Anxiety On Effort And Exercise Participation In College Students, Trellony M. Irwin

Honors Theses

The purpose of this study was to investigate whether an individual’s perception of others influenced exercise participation and effort in women and men participants involved in exercise classes in a campus recreation facility. The study consists of a survey that utilizes the Social Physique Anxiety Scale, the Ratings of Perceived Exertion (RPE) Scale, and demographic information. Results revealed that participants’ social physique anxiety accounted for roughly 30% of the variance in self-reported physical activity. However, when it came to participant effort during the group exercise classes, there was only roughly 2% variance between social physique anxiety and RPE. Further, the …

Salinity Tolerance Of Gambusia Affinis, Sarah Rubelowsky

Honors Theses

Developmental plasticity refers to changes during development as a result of environmental contributions. Salinity is a varying environmental condition in freshwater and estuarine habitats that can mediate developmental plasticity in Gambusia affinis, which can increase their tolerances as an invasive species. For my study, estuarine and freshwater populations of Gambusia affinis were sampled in March of 2017 using dip nets. Both populations were then brought back to the laboratory where pregnant females were acclimated to three different salinities (0‰, 15‰, 25‰) until they gave birth. I predicted that the estuarine population of Gambusia affinis would have a greater salinity tolerance …

The Effect Of Normal Helmet Degradation In Division I Football Players Over The Period Of One Half Of A Competitive Season, Matthew Mcmullan

Honors Theses

The purpose of this thesis is to evaluate the effect of normal helmet use by athletes from a Division I football team over the period of the first half of a competitive season upon the impact performance characteristics as measured by peak g values obtained through the application of the NOCSAE drop impact testing protocol. The goal of this research is to determine if one half of a season is enough time exposure to result in a significant decrease in a helmet’s performance quality. This study tracks changes in performance in overall function, as well as the function of each …

Elucidating The Interplay Between Sodium Selenite On The Tick Amblyomma Maculatum Selenoprotein Gene Expression, Afnan M. Beauti

Honors Theses

Selenium (Se) is an element recognized as an essential micronutrient in eukaryote organisms. Selenoproteins contain selenium as selenocysteine, the 21st amino acid. Selenium plays a role in cell growth and functioning. At low concentrations, it can induce growth and at high concentrations, it can cause a cell to stop growing and potentially have toxic effects on the cell and organism. When selenium levels are high, oxidative stress results by the production of reactive oxidative species. Selenoproteins, however, can aid the antioxidant response in the cell. Ticks are arthropods of interest, as they are one of few that contain many selenogenes, …

Effects Of Temperature Gradient, Substrate Composition, And Canopy Cover On The Spatial Distribution Of Topminnow Species: Fundulus Notatus And Fundulus Olivaceus, Austin M. King

Honors Theses

Hybrid zones are locations where two interbreeding species coexist and hybridize. The spatial distribution of ecologically similar species is of primary interest in understanding the formation and stability of hybrid zones. These hybrid zones are of significance as they allow for insight into how speciation occurs naturally within the environment. A variety of factors may play a role in determining the spatial distributions of species within hybrid zones. Examples of these factors include variations in temperature gradients, substrate composition, and changes in canopy cover. Fundulus olivaceus and Fundulus notatus are generally found within upstream and downstream habitats respectfully. These habitats …

Phylogenetic Relationships Of The Genera Of Achariaceae Based On Analyses Of Morphological And Dna Data, Corey L. Pagart

Honors Theses

Achariaceae are a mostly tropical family of flowering plants consisting of about 29 genera and 150 species of trees and shrubs. Although they are closely related to passionflowers (Passifloraceae), violets (Violaceae), and willows and cottonwoods (Salicaceae), phylogenetic relationships of the genera remain unclear because the only studies have been focused on particular genera or had limited sampling. Few studies of the family in general have been conducted, except for some on species that produce chaulmoogra oil, a commonly used historical treatment for leprosy. For my study, I investigated the relationships of the genera within the family using morphological and molecular …

The Effects Of Including Physical Activity In Classrooms With Children With Language Disorders, Shelby E. Auer

Honors Theses

As obesity becomes a rising concern in the United States and the importance of high test scores continues to add stress to students, physical activity opportunities are beginning to become less frequent in schools. The Center for Disease Control reports a total of 12.7 million children were classified as obese from 2011 to 2012 (CDC, 2015). While many researchers have also acknowledged this trend, the majority of studies focus on the correlation of physical activity in the classroom with increases in fitness and cognitive functioning levels among average population students, much like the research done by Webster, Russ, Vazou, Goh, …

Phylogenetic Relationships Of Salicaceae Based On Analyses Of Nuclear Dna Data, John Marshall Diffey

Honors Theses

Salicaceae are an economically and ecologically important family of flowering plants. The family includes willows and cottonwoods and was recently enlarged to include a large number of tropical species formerly placed in the family Flacourtiaceae. Relationships of these tropical relatives to willows and cottonwoods have been explored at a basic level using morphology and plastid DNA data, but to date no molecular phylogenies have been constructed with significant sampling of nuclear DNA, which sometimes results in a different picture of relationships because of its biparental inheritance. For this project, I sampled one region of nuclear DNA (GBSSI) across the family …

Effects Of Marsh Fragmentation And Patch Area On Fish And Nekton Assemblages In Mississippi, Jennifer R. Main

Honors Theses

Fragmentation divides continuous habitat into smaller patches. Fragmentation can also produce smaller populations of species, because fragmentation can split a population into smaller groups. Both terrestrial and aquatic ecosystems suffer from fragmentation. There are a variety of fishes that live along marsh, each depending upon the marsh for protection, food, and sometimes even competition. Species richness of fishes can be altered due to marsh fragmentation. I predict islands with a larger patch index should have a higher species richness of fishes. For this study, fish assemblages at 20 islands of marsh were sampled in the months of June and July …

Generation Of Mutant Snap-23 To Arrest Mast Cell Degranulation At Trans-Snare Complex Formation, Suzette Wafford-Turner

Honors Theses

Regulated secretion of pro-inflammatory molecules (e.g, histamines, proteases) from mast cells plays critical roles in immunity, allergic reactions, cardiovascular disease and cancer. These molecules are stored in secretory granules inside the cell and are rapidly released into the extracellular environment when mast cells are activated. It is known that mast cell degranulation depends upon membrane anchored SNAREs (soluble N-ethylmaleidimide-sensitive factor attachment protein receptors) and accessory proteins that form the trans-SNARE complex, a 4 helical bundle central to exocytic fusion. There are three SNARE proteins that contribute to the 4-helical bundle during exocytosis; Syntaxin and VAMP proteins each provide one helix …

Altering The Structure Of Carboxysomal Carbonic Anhydrase Csosca To Determine The Necessity Of The N-Terminal Domain In Csosca Function, Dana L. Dillistone

Honors Theses

In this project, a DNA construct was designed and developed to remove the first fifty amino acids of the CsoSCA protein in the chemolithotrophic bacterium Halothiobacillus neapolitanus. The csoS3 gene codes for a carbonic anhydrase enzyme (CsoSCA) that is unique to a structure called a carboxysome. Carboxysomes are polyhedral microcompartments where carbon fixation is housed. The carbonic anhydrase is a shell-associated protein that improves the catalytic efficiency of ribulose-bisphosphate carboxylase/oxygenase (RuBisCO), the enzyme that catalyzes the fixation of carbon. By deleting the first fifty amino acids of the carbonic anhydrase, the necessity of the amino acids in carboxysome function was …

Laterality Preferences Completing A Tool-Use Task In Asian Small-Clawed Otters (Aonyx Cinereus), Stephanie E. James

Honors Theses

Laterality is defined as the occurrence of each cerebral hemisphere having asymmetric control over the different sides of the body, leading to preferences to use one side of the body over the other for various behaviors. Many types of laterality exist, but handedness is the form that is most predominantly assessed. Handedness in animals is of special interest to laterality researchers, as humans were once thought to be the only species that exhibited lateralized hand preferences. The aim of the current study was to determine if Asian small-clawed otters exhibit significant paw preferences in behaviors associated with completing a tool-use …

Effect Of Physical Activity On Quality Of Life For College Students: A Comparative Gender Study, Sarah J. Boozer

Honors Theses

Purpose

The purpose of this study was to examine the relationship between physical activity levels in college students and health related quality of life scores. Additional analysis was used to determine whether more of a relationship exists between female or male students’ in terms of activity versus quality of life.

Methods

Students for this study were gathered through their involvement in kinesiology based organizations on campus. Two surveys were utilized, the Global Physical Activity Questionnaire and the Health Related Quality of Life survey. Students were also asked to record their gender, which allowed for the second analysis. After scoring the …

The Preparation Of Synthetic Myod Mrna For Cellular Differentiation And Innate Immune Response Downstream Application, James D. Grenn

Honors Theses

The development of therapeutic immune responses from the manipulation of embryonic stem cells (ESCs) via induction of synthetic RNA with 5’ capped and 3’ poly(A)tailed ends would lead to development of stem cell therapy. A necessary step in attaining such a goal is to first produce an mRNA transcript from a plasmid containing the open reading frame (ORF) for a transcription factor for cellular activation.

In this research, the DNA plasmid pMD4 encoding MyoD was transcribed into synthetic mRNA. The plasmid was first amplified using Polymerase Chain Reaction (PCR), and analyzed using gel electrophoresis. The amplified template was purified via …

Characterization Of P43lgrn-3, Kelly G. Hill

Honors Theses

Alzheimer’s Disease (AD) is a progressive neurodegenerative disorder characterized by extensive memory loss and cognitive deficits, which occur due to severe neuronal loss. Two hallmark lesions, intracellular neurofibrillary tangles composed of hyperphosphorylated tau and extracellular neuritic plaques formed by the aggregation of amyloid-β (Aβ), are responsible for the progressive neuronal loss seen in AD brains. Neurotoxic Aβ aggregates are also known to cause inflammation within the brain. It has recently come to light that severe and acute inflammation, such as seen in traumatic brain injury (TBI), may also lead to AD-type dementia. This has raised the question whether some of …

The Regulatory Relationship Of Transcriptional Regulators Msab And Cody In Capsule Production In Staphylococcus Aureus, Brittany L. Trunell

Honors Theses

There are many transcriptional regulators found in pathogenic Staphylococcus aureus. Many of these regulators are essential for the organism’s ability to switch from commensal form to the virulent pathogenic form. One of these main regulators is CodY. This regulator has been shown to be responsive to nutrient availability during phases of growth. Additionally, we have recently found that MsaB, the only protein coding ORF of the msaABCR operon, is a putative co-regulator of capsule along with CodY. To explore these regulator interactions, we produced mutations of codY and msaABCR individually and a double mutation of msaABCR/ codY. We have observed …

Production Of Antibodies For Specific Detection Of Dengue Virus-2 By Surface Enhanced Raman Spectroscopy, E. Ashley Thompson

Honors Theses

Dengue Virus (DENV) is a flavivirus spread by mosquitoes that are endemic in tropic and subtropic climates. It is the causative pathogen of Dengue Fever, Dengue Hemorrhagic Fever, and Dengue Shock Syndrome. Besides mosquito transmission, DENV can also be transmitted through blood transfusion. Due to the high expense and expertise needed to run tests like ELISA and Polymerase Chain Reaction (PCR) based-assays for DENV detection, a rapid, sensitive and cost effective gold nanoparticles mediated surface-enhanced Raman spectroscopy (SERS) assay has been developed as an alternative assay to detect DENV. In this project, I successfully produced a monoclonal antibody that specifically …

Influence Of Compression Socks On Unilateral Balance In Females, Alex E. Carlson

Honors Theses

The application of compression garments for various purposes has become popular in recent years. Research regarding the effectiveness of these garments has shown an increase in variables such as increased blood flow, decreased post-exercise soreness, and increased joint position awareness. However, little research is available concerning their possible use to increase joint stability. This research examined the efficacy of graduated compression stockings (GCS) to increase proprioceptive feedback from the ankle joint. It was hypothesized that continuous stimulation of sensory receptors in the ankle region by use of GCS would improve an individual’s static unilateral balance. Seventeen females (age: 20 +/- …