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Full-Text Articles in Life Sciences

Examining Interactions Between Opsins And Carotenoid Biosynthetic Proteins In Halophilic Archaea, Alexandru M. Plesa Jan 2017

Examining Interactions Between Opsins And Carotenoid Biosynthetic Proteins In Halophilic Archaea, Alexandru M. Plesa

Honors Theses

Organisms have evolved numerous specialized molecules for constantly responding to environmental changes. Examples of such molecules are the light-driven proton-pump rhodopsins, such as bacteriorhodopsin (BR) and cruxrhodopsin (cR), and the carotenoid pigments, such as retinal and bacterioruberin. In halophilic Archaea, retinal can covalently bind bacterioopsin (BO) and cruxopsin (CO) to form the corresponding protein complexes, and its biosynthesis is indirectly controlled by the activity of the lycopene elongase (Lye) enzyme, which converts lycopene, a retinal precursor, to a form of bacterioruberin. Interestingly, opsins were shown to inhibit the activity of Lye, thereby promoting retinal biosynthesis and indirectly regulating the apoprotein-cofactor …


Protein Kinase Activity Toward Taabf1 In Imbibing Grains, Taylor P. Enrico Jan 2017

Protein Kinase Activity Toward Taabf1 In Imbibing Grains, Taylor P. Enrico

Honors Theses

The hormones gibberellin and abscisic acid are essential for plant responses to changing environmental conditions, and can send opposing signals. In wheat, the transcription factor TaABF1 plays an important role at the intersection of a gibberellin-induced/abscisic acid-suppressed pathway. When gibberellin dominates, the GA-induced gene, Amy32b, is transcribed. When abscisic acid is dominant, TaABF1 is active and it downregulates GA-induction of Amy32b, while promoting ABA-induced transcription of the gene HVA1. The activity of TaABF1 is thought to be regulated by post-translational phosphorylation at key serine residues. In this study, to determine TaABF1 phosphorylation by wheat kinases, we purified recombinant …


Vein Patterning During Juvenile Wing Development In Oncopeltus Fasciatus And Jadera Haematoloma, William R. Simmons Jan 2017

Vein Patterning During Juvenile Wing Development In Oncopeltus Fasciatus And Jadera Haematoloma, William R. Simmons

Honors Theses

Wings are a defining characteristic of all pterygote insects and are agreed to originate from a single common winged ancestor. However, essentially nothing is known about the molecular mechanisms that regulate wing development and patterning outside of Endopterygota, and the vast majority of this knowledge comes solely from Drosophila melanogaster. There is reason to suspect that the drastic developmental differences that exist between Endopterygota and Exopterygota have resulted in changes in the mechanisms, timings, or even genes that govern wing development. This study examined the roles of several genes known to be involved in D. melanogaster wing development and patterning …


Crops And Controversy: Industry’S Role In The Gmo Debate, Carina Wallack Jan 2017

Crops And Controversy: Industry’S Role In The Gmo Debate, Carina Wallack

Honors Theses

The use of genetically modified crops (also called GM crops, genetically modified organisms, or GMOs for short) has become a highly contested issue. New genetic modification technologies offer a variety of advantages for improving agricultural efficiency. However, some scientists argue that the safety testing conducted thus far does not provide enough information and worry about possible health and ecological risks. Private industry sponsors a great deal of research on genetically modified crops. As the international controversy regarding the use of GMOs has unfolded, the very companies responsible for commercializing genetically modified crops have gained substantial influence in the resulting scientific …


Temporal Changes In The Larval Placopecten Magellanicus Population In A Small-Scale Fishery Closure Area In Coastal Maine, Usa, Jessica P. Batchelder Jan 2017

Temporal Changes In The Larval Placopecten Magellanicus Population In A Small-Scale Fishery Closure Area In Coastal Maine, Usa, Jessica P. Batchelder

Honors Theses

The Midcoast Maine Collaborative Scallop Project was established in 2013 by fishers, scientists, and policy makers to determine if a small-scale closure area could restore the local Atlantic sea scallop (Placopecten magellanicus) population to an area in coastal Maine that previously supported high scallop densities. These stakeholders established a three by one mile closure area in the Lower Muscle Ridge Channel to assess the response of the adult and larval scallop populations. Understanding the larval dynamics in a closure area is key to evaluating the recovery potential of the population and for future population stock levels. This study …