Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Beryllium Nitrate Inhibits Fibroblast Migration To Disrupt Epimorphic Regeneration, Adam B. Cook, Ashley W. Seifert
Beryllium Nitrate Inhibits Fibroblast Migration To Disrupt Epimorphic Regeneration, Adam B. Cook, Ashley W. Seifert
Biology Faculty Publications
Epimorphic regeneration proceeds with or without formation of a blastema, as observed for the limb and skin, respectively. Inhibition of epimorphic regeneration provides a means to interrogate the cellular and molecular mechanisms that regulate it. In this study, we show that exposing amputated limbs to beryllium nitrate disrupts blastema formation and causes severe patterning defects in limb regeneration. In contrast, exposing full-thickness skin wounds to beryllium only causes a delay in skin regeneration. By transplanting full-thickness skin from ubiquitous GFP-expressing axolotls to wild-type hosts, we demonstrate that beryllium inhibits fibroblast migration during limb and skin regeneration in vivo. Moreover, …
Comparative Analysis Of Ear-Hole Closure Identifies Epimorphic Regeneration As A Discrete Trait In Mammals, Thomas R. Gawriluk, Jennifer Simkin, Katherine L. Thompson, Shishir K. Biswas, Zak Clare-Salzler, John M. Kimani, Stephen G. Kiama, Jeramiah James Smith, Vanessa O. Ezenwa, Ashley W. Seifert
Comparative Analysis Of Ear-Hole Closure Identifies Epimorphic Regeneration As A Discrete Trait In Mammals, Thomas R. Gawriluk, Jennifer Simkin, Katherine L. Thompson, Shishir K. Biswas, Zak Clare-Salzler, John M. Kimani, Stephen G. Kiama, Jeramiah James Smith, Vanessa O. Ezenwa, Ashley W. Seifert
Biology Faculty Publications
Why mammals have poor regenerative ability has remained a long-standing question in biology. In regenerating vertebrates, injury can induce a process known as epimorphic regeneration to replace damaged structures. Using a 4-mm ear punch assay across multiple mammalian species, here we show that several Acomys spp. (spiny mice) and Oryctolagus cuniculus completely regenerate tissue, whereas other rodents including MRL/MpJ ‘healer’ mice heal similar injuries by scarring. We demonstrate ear-hole closure is independent of ear size, and closure rate can be modelled with a cubic function. Cellular and genetic analyses reveal that injury induces blastema formation in Acomys cahirinus. …
Analogous Cellular Contribution And Healing Mechanisms Following Digit Amputation And Phalangeal Fracture In Mice, Lindsay A. Dawson, Jennifer Simkin, Michelle Sauque, Maegan Pela, Teresa Palkowski, Ken Muneoka
Analogous Cellular Contribution And Healing Mechanisms Following Digit Amputation And Phalangeal Fracture In Mice, Lindsay A. Dawson, Jennifer Simkin, Michelle Sauque, Maegan Pela, Teresa Palkowski, Ken Muneoka
Biology Faculty Publications
Regeneration of amputated structures is severely limited in humans and mice, with complete regeneration restricted to the distal portion of the terminal phalanx (P3). Here, we investigate the dynamic tissue repair response of the second phalangeal element (P2) post amputation in the adult mouse, and show that the repair response of the amputated bone is similar to the proximal P2 bone fragment in fracture healing. The regeneration-incompetent P2 amputation response is characterized by periosteal endochondral ossification resulting in the deposition of new trabecular bone, corresponding to a significant increase in bone volume; however, this response is not associated with bone …