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Full-Text Articles in Life Sciences

Histology And Ultrastructure Of Montastraea Cavernosa And Porites Astreiodes During Regeneration And Recruitment: Anthropogenic Stressors And Transplant Success, Dorothy-Ellen A. Renegar Nov 2016

Histology And Ultrastructure Of Montastraea Cavernosa And Porites Astreiodes During Regeneration And Recruitment: Anthropogenic Stressors And Transplant Success, Dorothy-Ellen A. Renegar

D. Abigail Renegar

Corals combine photosynthesis and calcification in an intricate and delicately balanced relationship to form large biomineralized structures that are dominant features of tropical coastlines worldwide. Coral reefs have great scientific and economic importance but have recently experienced widespread decline attributed to increasing anthropogenic pressure on reef systems. Physical damage events, such as ship groundings, when coupled with existing nutrient stress and changing global climate present a poor outlook for successful natural recovery of reef communities. The main goal of the proposed research is to better understand how environmental factors, both local and global, affect the coral holobiont and influence overall …


Reproductive Parameters Of Coastal Pelagic Fishes, Sonia Ahrabi-Nejad, David W. Kerstetter, Patricia Blackwelder, Dorothy-Ellen A. Renegar Nov 2016

Reproductive Parameters Of Coastal Pelagic Fishes, Sonia Ahrabi-Nejad, David W. Kerstetter, Patricia Blackwelder, Dorothy-Ellen A. Renegar

D. Abigail Renegar

The ability to manage a fish stock relies on an understanding of life history characteristics and basic biology of the species. Numerous age-growth studies are facilitated by the relative ease of ageing fishes through hard-part analyses. Determining reproductive parameters for fish populations is just as important for stock assessments and management, and histological examination of gonads provides the most accurate determination of fecundity and spawning periods. However, research in this area is limited. Coastal pelagic fishes are often targeted commercially and recreationally due to their easy access by private vessels. The objective of this study is to provide baseline data …


Ultrastructural And Histological Analysis Of Dark Spot Syndrome In Siderastrea Siderea And Agaricia Agaricites, Dorothy-Ellen A. Renegar, Patricia Blackwelder, J. D. Miller, D. J. Gochfeld, Alison L. Moulding Nov 2016

Ultrastructural And Histological Analysis Of Dark Spot Syndrome In Siderastrea Siderea And Agaricia Agaricites, Dorothy-Ellen A. Renegar, Patricia Blackwelder, J. D. Miller, D. J. Gochfeld, Alison L. Moulding

D. Abigail Renegar

Dark Spot Syndrome (DSS) typically manifests in scleractinian corals as lesions of varying color, size, shape and location that can result in skeletal changes and tissue death. A causative agent for DSS has not yet been identified. The objective of this study was histological and ultrastructural comparison of the cellular and skeletal characteristics of DSS-affected and healthy Siderastrea siderea and Agaricia agaricites. The greater resolution possible with transmission electron microscopy (TEM) revealed microbial activity and tissue changes not resolvable utilizing histology. DSS-affected tissue had less integrity, with increasing cellular degradation and vacuolization. A high concentration of electron dense inclusions, …


Effect Of Nutrient Enrichment And Elevated Co2 Partial Pressure On Growth Rate Of The Zooxanthellate Coral Acropora Cervicornis., Dorothy-Ellen A. Renegar Nov 2016

Effect Of Nutrient Enrichment And Elevated Co2 Partial Pressure On Growth Rate Of The Zooxanthellate Coral Acropora Cervicornis., Dorothy-Ellen A. Renegar

D. Abigail Renegar

Trends of increasing coastal eutrophication and atmospheric pCO2 require investigation to predict the combined effects on coral and reef condition and growth. Increases in nutrient concentrations have been observed over the past several decades in a number of reef systems, and it has been predicted that this will adversely affect coral growth rates. The species targeted by this research, Acropora cervicornis, is among the most important reef-builders in the Caribbean and has suffered widespread mortality in southern Florida. Approximately 192 branch tips were harvested from two local populations of A. cervicornis and maintained in the laboratory, where the growth rate …


Coral Ultrastructural Response To Elevated Pco2 And Nutrients During Tissue Repair And Regeneration, Dorothy-Ellen A. Renegar, Patricia Blackwelder, Alison L. Moulding Nov 2016

Coral Ultrastructural Response To Elevated Pco2 And Nutrients During Tissue Repair And Regeneration, Dorothy-Ellen A. Renegar, Patricia Blackwelder, Alison L. Moulding

D. Abigail Renegar

Corals and coral reefs have recently experienced widespread decline attributed to anthropogenic pressure on reef systems. Studies have demonstrated that nutrient and pCO2 stress effect coral growth and calcification, but study of specific effects on coral tissue is lacking. The objective of this research was to examine wound healing in corals and how it is affected by exposure to elevated nutrients and pCO2. Coral tissue repair and regeneration during wound healing in Montastraea cavernosa and Porites astreoides were assessed histologically and ultrastructurally by examining colony fragments exposed to elevated nitrate, phosphate, and pCO2. In M. cavernosa, tissue repair was facilitated …


Acute And Sub-Acute Toxicity Of The Polycyclic Aromatic Hydrocarbon 1-Methylnaphthalene To The Shallow-Water Coral Porites Divaricata: Application Of A Novel Exposure Protocol, Dorothy-Ellen A. Renegar, Nick Turner, Bernhard Riegl, Richard E. Dodge, Anthony H. Knap, Paul Schuler Nov 2016

Acute And Sub-Acute Toxicity Of The Polycyclic Aromatic Hydrocarbon 1-Methylnaphthalene To The Shallow-Water Coral Porites Divaricata: Application Of A Novel Exposure Protocol, Dorothy-Ellen A. Renegar, Nick Turner, Bernhard Riegl, Richard E. Dodge, Anthony H. Knap, Paul Schuler

D. Abigail Renegar

Previous research evaluating hydrocarbon toxicity to corals and coral reefs has generally focused on community-level effects, and results often are not comparable between studies because of variability in hydrocarbon exposure characterization and evaluation of coral health and mortality during exposure. Toxicity of the polycyclic aromatic hydrocarbon 1-methylnaphthalene to the coral Porites divaricata was assessed in a constant exposure toxicity test utilizing a novel toxicity testing protocol uniquely applicable to shallow-water corals, which considered multiple assessment metrics and evaluated the potential for post-exposure mortality and/or recovery. Acute and subacute effects (gross morphological changes, photosynthetic efficiency, mortality, and histologic cellular changes) were …