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Detection And Quantification Of Airborne Conidia Of Fusarium Circinatum, The Causal Agent Of Pine Pitch Canker, From Two California Sites Using A Real Time-Pcr Approach Combined With A Simple Spore Trapping Method, Wolfgang Schweigkofler, Kerry O'Donnell, Matteo Garbelotto
Detection And Quantification Of Airborne Conidia Of Fusarium Circinatum, The Causal Agent Of Pine Pitch Canker, From Two California Sites Using A Real Time-Pcr Approach Combined With A Simple Spore Trapping Method, Wolfgang Schweigkofler, Kerry O'Donnell, Matteo Garbelotto
Wolfgang Schweigkofler
Pinus radiata (Monterey pine), a tree native to coastal California and Mexico, is widely planted worldwide for timber production. A major threat to Monterey pine plantations is the fungal disease pine pitch canker, caused by Fusarium circinatum (Hypocreales). We present a novel trapping approach using filter paper in combination with a rapid molecular method to detect the presence of inoculum in the air. The assay is also useful for diagnosing the presence of the pathogen on plants. The test is based on the F. circinatum specific primer pair CIRC1A-CIRC4A, which amplifies a 360-bp DNA fragment in the intergenic spacer region …
Resistance Inducers And Plant Growth Regulators Show Only Limited And Transient Effects On Infection Rates, Growth Rates And Symptom Expression Of Apple Trees Infected With ‘Candidatus Phytoplasma Mali’, Silva Schmidt, Sanja Baric, Maya Massenz, Thomas Letschka, Valerie Vanas, Manfred Wolf, Christine Kerschbamer, Roland Zelger, Wolfgang Schweigkofler
Resistance Inducers And Plant Growth Regulators Show Only Limited And Transient Effects On Infection Rates, Growth Rates And Symptom Expression Of Apple Trees Infected With ‘Candidatus Phytoplasma Mali’, Silva Schmidt, Sanja Baric, Maya Massenz, Thomas Letschka, Valerie Vanas, Manfred Wolf, Christine Kerschbamer, Roland Zelger, Wolfgang Schweigkofler
Wolfgang Schweigkofler
The effects of four commercially available bio-active compounds on the infection rates, symptom expression and growth rates of apple trees (Malus × domestica Borkh.) cv. Golden Delicious infected with ‘Candidatus Phytoplasma mali’ (the so-called Apple Proliferation phytoplasma or AP) were tested over a three-year period under controlled conditions. Post-infection treatments using Bion® (active ingredient: Acibenzolar-S-Methyl), Messenger® (Harpin protein), Regalis® (Prohexadione-Ca) and Dormex® (Cyanamide) had no significant effect on infection rates. Terminal growth of apple trees (grown as one-shoot pruned trees) was increased significantly by AP infection; Prohexadione-Ca was the only compound which had a significant (inhibiting) effect on the growth …