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Full-Text Articles in Life Sciences
Sequence Annotation & Designing Gene-Specific Qpcr Primers (Computational), Ray A. Enke
Sequence Annotation & Designing Gene-Specific Qpcr Primers (Computational), Ray A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- Obtaining and annotating genomic DNA and mRNA sequence information
- Designing primers for quantitative PCR (qPCR) analysis of a cDNA library
Qpcr Primer Standard Curve Assay (Wet Lab) + Kegg Pathway Analysis (Computational), Ray A. Enke
Qpcr Primer Standard Curve Assay (Wet Lab) + Kegg Pathway Analysis (Computational), Ray A. Enke
Ray Enke Ph.D.
This class tested protocol will guide students through the steps for the following activities:
- analyzing qPCR standard curve data to determine primer efficiency
- analyzing differential gene expression experimental qPCR data
- applying KEGG pathway analysis of selected candidates genes