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Full-Text Articles in Life Sciences

Nematicidal Activity Of Extracts From Phytolacca Americana On Five Plant-Pathogenic Nematode Species Of Economic Importance, Saleh Ahmed Dec 2015

Nematicidal Activity Of Extracts From Phytolacca Americana On Five Plant-Pathogenic Nematode Species Of Economic Importance, Saleh Ahmed

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Plant-parasitic nematodes cause significant losses to plant agriculture annually. The limited availability and high cost of synthetic nematicides, along with the environmental risks associated with their use, have created a renewed interest in the search for alternative management tactics. One such alternative tactic is the use of plant extracts. The focus of this research is the evaluation of the nematicidal activity of water extracts from Pokeweed (Phytolacca americana). In vitro and greenhouse tests were performed to evaluate the effects of the extracts on five species of plant-pathogenic nematodes of economic importance: Aphelenchoides fragariae, Ditylenchus dispsaci, Meloidogyne incognita, Pratylenchus penetrans, and …


Chinese Tallow Invasion In Maritime Forests: Understand Invasion Mechanism And Develop Ecologically-Based Management, Lauren Susan Pile Dec 2015

Chinese Tallow Invasion In Maritime Forests: Understand Invasion Mechanism And Develop Ecologically-Based Management, Lauren Susan Pile

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Invasion by highly aggressive, non-native, invasive plants is a significant threat to management and conservation priorities as these plants can transform ecosystem functions and processes. In this study, I investigated the non-native, invasive tree, Chinese tallow (Triadica sebifera (L.) Small) in the maritime forests of Parris Island, South Carolina. I studied the role of land-use history and modern forest disturbance in facilitating the invasion of Chinese tallow. I found that stands previously cleared for agriculture and reforested with slash pine (Pinus elliottii Englem.) since the 1970s had significantly more Chinese tallow stems than stands that remained forested since …


Effects Of Semi-Rigid Plastic Tray Geometry On Thermal Processing And Quality Factors In Retorted Foods, Curtis Stowe Dec 2015

Effects Of Semi-Rigid Plastic Tray Geometry On Thermal Processing And Quality Factors In Retorted Foods, Curtis Stowe

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This research studied the effect of packaging geometry changes on heat penetration and quality attributes of a food system processed in a rotary retort vessel. Studies were conducted to determine the effect of package geometry in a rotary retort on heat penetration, analytical, and physical properties of a model food system processed at optimum conditions. Additionally, heat mapping for each shape was used to determine the heating profiles during retorting. Retort-able trays were filled with a tomato based food simulate and thermally processed in a water immersion, automated batch retort system (ABRS) using rotational speeds of 6 RPM and 11 …


Development And Implementation Of The Generations Eating Together Through Cooking (G.E.T.T. Cooking) Curriculum And Its Effects On An Inter-Generational Population: A Pilot Study, Elizabeth Ramirez Dec 2015

Development And Implementation Of The Generations Eating Together Through Cooking (G.E.T.T. Cooking) Curriculum And Its Effects On An Inter-Generational Population: A Pilot Study, Elizabeth Ramirez

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The obesity epidemic continues to be a problem both in the US and worldwide. A number of factors have been attributed including: frequency of fast-food consumption, increased portion sizes, increased consumption of sugar-sweetened beverages, increased sedentary lifestyles, lack of nutrition knowledge, and lack of cooking skills. Nutrition intervention programs continue to be created providing innovative and creative ways of educating the public with pertinent knowledge and skills necessary for improving healthful behaviors; however, few programs focus on cooking skill development within a familial environment. The Generations Eating Together Through Cooking (G.E.T.T. Cooking) program was created by the lead researcher as …


Development And Validation Of A Novel In Vitro Model For The Assessment Of Heterocellular Interactions Mediated By Connexin43, Emily Ongstad Dec 2015

Development And Validation Of A Novel In Vitro Model For The Assessment Of Heterocellular Interactions Mediated By Connexin43, Emily Ongstad

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The injury border zone (IBZ), a region of transitional tissue between intact myocardium and the ischemic area, is often the site of lethal reentrant arrhythmia generation in post-myocardial infarction (MI) patients. Disruption to normal connexin43 (Cx43) localization at the intercalated disc (ID), separation of myocytes by activated fibroblasts and deposited scar tissue are thought to be factors that render the IBZ a pro-arrhythmic substrate, though there is a current need to better understand these changes so directed therapies can be developed. There are no clinically available therapies focused on the mechanistic changes in the IBZ. Additionally, generation of new compounds …


Evaluation Of Blue Tilapia (Oreochromis Aureus) For Duckweed (Lemna Minor) Control In South Carolina's Private Waters, William Heaton Dec 2015

Evaluation Of Blue Tilapia (Oreochromis Aureus) For Duckweed (Lemna Minor) Control In South Carolina's Private Waters, William Heaton

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ABSTRACT We investigated the potential of Blue Tilapia (Oreochromis aureus) as a means for biological control of duckweed in agricultural impoundments, and assessed the potential for naturalization of this non-native fish in coastal regions of South Carolina. Additionally, we studied growth characteristics and nutrient accumulation abilities of duckweed (Lemna minor) growing in agricultural impoundments in the piedmont and coastal plain. Observations of tank feeding trials indicated juvenile Blue Tilapia consumed 36 – 86% of their body weight in fresh duckweed every 24 hours. Tilapia were stocked in agricultural impoundments in the piedmont and coastal plain during the 2012 and 2013 …


Web-Based Nutrition Education Intervention For African American Women Using The Theory Of Planned Behavior, Joyce Senior Angulo Dec 2015

Web-Based Nutrition Education Intervention For African American Women Using The Theory Of Planned Behavior, Joyce Senior Angulo

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African American women have been disproportionately impacted for decades by the obesity epidemic, which frequently leads to severe chronic diseases such as cardiovascular disease, diabetes and hypertension. This increase in chronic diseases amongst African American women is the motivation for the design of a culturally tailored nutrition education (NE) program that combines a strong theoretical base with the use of technology such as the Internet, since its usage within the college aged African American community has rapidly increased. Little is known about the variables that motivate behavior change in regards to eating and physical activity habits among college aged African …


Microrna-Mediated Plant Development And Response To Environmental Stress In Perennial Grasses, Shuangrong Yuan Dec 2015

Microrna-Mediated Plant Development And Response To Environmental Stress In Perennial Grasses, Shuangrong Yuan

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World population will pass nine billion by 2050, while the agricultural land area will not increase dramatically in the coming decades. To meet the ever-increasing food demand, genetically engineered crops have been rapidly adopted for crop productivity. MicroRNAs have become increasingly attractive as targets in crop genetic modification due to their regulatory role in fine-tuning many essential biological processes. My research explores the potentials of microRNA528 (miR528) and miR396 for use in genetic modifications of the important agronomic traits of plant development, abiotic stress response, and/or flowering time control in an economically and environmentally important perennial monocot species, creeping bentgrass …


Weeds, Nitrogen, And Yield: Measuring The Effectiveness Of An Organic No-Till System, David Robb Dec 2015

Weeds, Nitrogen, And Yield: Measuring The Effectiveness Of An Organic No-Till System, David Robb

All Theses

Previous research in the mid-Atlantic and midwestern USA has identified advantages and drawbacks of "organic no-till" vegetable production, but few studies have been conducted in the warmer southeastern region. The purpose of this study was to examine the effects of tillage [no-till (NT) vs. conventional tillage (CT) of a cereal rye/crimson clover cover crop] and three nitrogen fertilization rates on organic tomato (Solanum lycopersicum L.) and summer squash (Cucurbita pepo L.) yield, weed suppression, and soil N dynamics in two years in a soil series in Clemson, SC. Squash yields were similar between tillage treatments in both years. NT tomato …


Development And Implementation Of Hay Yield Monitoring Technology, Henry Ramsey Dec 2015

Development And Implementation Of Hay Yield Monitoring Technology, Henry Ramsey

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Three independent technologies related to hay yield monitoring were developed and studied. One technology involved comparison of infrared and ultrasonic sensors on a self-propelled forage harvester. Sensor response related to grass height and was used to estimate yield. Plots that were harvested ranged from 20-40 ft. in length while a bin mounted on the back of the mower collected the crop for weighing and sampling. The infrared sensors data demonstrated accuracies across plots between 5.9% and 9.5% error. The infrared sensors quickly deteriorated and eventually proved to be useless for data acquisition. As for the ultrasonic sensors, they demonstrated similar …


Influence Of Exurban Neighborhoods On Riparian Vegetation And Stream Salamanders In The Southern Appalachian Mountains, Nathaniel Weaver Dec 2015

Influence Of Exurban Neighborhoods On Riparian Vegetation And Stream Salamanders In The Southern Appalachian Mountains, Nathaniel Weaver

All Theses

The southern Appalachian Mountains have experienced large population growth and a change in land use in the past 30 years. The majority of development has been low density, suburban land, known as exurban development. Lotic Systems and riparian areas are severely degraded by conversion from urban to rural land uses. The long-term effects of exurbanization on riparian vegetative communities and stream salamanders in the southern Appalachian Mountains are not well known. We sought to determine if vegetative community composition and structure change with time since neighborhood development or with the amount of impervious surface within the watershed. In order to …


Management And Impacts Of Wild Hogs (Sus Scrofa) In South Carolina, Susan Sullivan Dec 2015

Management And Impacts Of Wild Hogs (Sus Scrofa) In South Carolina, Susan Sullivan

All Theses

Wild hogs (Sus scrofa) are an invasive species that can damage native ecosystems, negatively impact native wildlife, and potentially act as disease reservoirs in the U.S. To better understand how natural resource professionals in South Carolina approach wild hog management, I conducted a web-based survey of natural resource professionals focused on aspects of wild hog impacts and management. Generally, there was agreement among natural resource professionals regarding the approaches used to control wild hog populations and their subsequent effectiveness. The majority of respondents indicated high priority impacts needing to be addressed in wild hog management were agricultural damage …


Development And Testing Of Supplemental Materials For An Undergraduate Healthy Food Product Development Curriculum, Breanne Halteman Dec 2015

Development And Testing Of Supplemental Materials For An Undergraduate Healthy Food Product Development Curriculum, Breanne Halteman

All Theses

ABSTRACT The goal of this research project was three-fold: (1) to follow up with students who had taken the Applied Interdisciplinary Product Development (AIPD) course two years prior to assess the long-term effect of the course on perceived self-confidence in product development skills, connectedness with the department, and preparedness to enter the industry; (2) to design, implement, and evaluate course materials educating undergraduate students about the subjects of herbs, spices, and sensory science; and (3) to evaluate the success of dissemination of a sophomore-level hybrid course on healthy food product development. A Subject Knowledge Assessment (SKA) was designed to measure …


Comparative Aquatic Toxicity Of A Commercial Naphthenic Acid And Processes For Mitigating Risks, Ciera Kinley Dec 2015

Comparative Aquatic Toxicity Of A Commercial Naphthenic Acid And Processes For Mitigating Risks, Ciera Kinley

All Theses

Comparative toxicity data can inform predictions of relative risk, and can be used to implement strategies for altering exposures to mitigate risk. Naphthenic acids (NAs) are a complex group of carboxylic acids that naturally occur in petroleum sources and energy-derived process waters (e.g. refinery effluents and oil sands process affected waters). These compounds are relatively persistent in water and can be a source of toxicity to aquatic organisms. In the first experiment of this thesis, responses of sentinel aquatic organisms to 7-d exposures of commercial (Fluka) NAs were measured (in terms of acute toxicity) to discern relative sensitivities. In terms …


The Detection And Characterization Of Some Viruses Infecting Blackberry And Cherry In South Carolina, Bindu Poudel Dec 2015

The Detection And Characterization Of Some Viruses Infecting Blackberry And Cherry In South Carolina, Bindu Poudel

All Dissertations

Three separate virus research projects were conducted. Blackberry yellow vein disease (BYVD), a disorder caused by virus complexes, has become a major threat to blackberry production in the United States, especially in the southeastern part of the country where blackberries are grown for the fresh market. More than 30 viruses have been found to be associated with the disease. Most of these induce no symptoms when infecting the plant alone. However, when more than a single virus is present in the host visible symptoms are displayed. The incidence of 6 different viruses (Blackberry yellow vein-associated virus, Blackberry virus Y, Blackberry …


Arsenic Inhibits P19 Stem Cell Differentiation By Altering Microrna Expression And Repressing The Sonic Hedgehog Signaling Pathway, Jui Tung Liu Dec 2015

Arsenic Inhibits P19 Stem Cell Differentiation By Altering Microrna Expression And Repressing The Sonic Hedgehog Signaling Pathway, Jui Tung Liu

All Dissertations

Arsenic is a naturally-occurring toxicant that exists in bedrock and can be leached into ground water. Humans can be exposed to arsenic via contaminated drinking water, fruit, rice or crops. Epidemiological studies have shown that arsenic is a developmental toxicant, and in utero exposure reduces IQ scores, verbal learning ability, decreases long term memory, and increases the likelihood of dying from a neurological disorder. Arsenic can also reduce birth weight, weight gain, and muscle function after an in utero exposure. Although the mechanism behind these physiological changes is not known, in vitro studies have shown that arsenic can reduce muscle …


Nutrition Education For Children Attending Preschool (Necap), Ibtehal Alsallaiy Dec 2015

Nutrition Education For Children Attending Preschool (Necap), Ibtehal Alsallaiy

All Dissertations

There is a need to educate children as young as 3-5 years old about nutrition. It is at this age when children start to develop their preferences for certain foods. The purpose of this research study was to; explore the perceptions, preferences, and dietary intake of the main food groups in preschool children, explore the availability, choices, and preparation of the main food groups by the care providers, design and present a nutrition education program to the children and their care providers. The average consumption of fruits during breakfast and lunch prior to the program was 51.6 %, 59.3 % …


Relationships Between Growth Rate And Gene Expression In Ruegeria Pomeroyi Dss-3, A Model Marine Alphaproteobacterium, Nattasha Vinas Aug 2015

Relationships Between Growth Rate And Gene Expression In Ruegeria Pomeroyi Dss-3, A Model Marine Alphaproteobacterium, Nattasha Vinas

All Theses

Microbes are important contributors to ecosystem processes such as biogeochemical cycling. Their activities vary, depending on environmental parameters such as carbon type and concentration, temperature, and salinity. Current estimates of in situ microbial growth is limited to gross estimates or rely on incubation based methods that disturb the natural state of the community. The goal of this master’s thesis is to develop molecular methods to directly assess microbial growth rates in the environment at the level of a taxonomic group. Here we grew Ruegeria pomeroyi DSS-3 under different temperatures (15 °C or 30 °C) and different carbon sources (yeast extract/tryptone, …


Estimating Tiger (Panthera Tigris) Prey Density Using Camera Traps And Fecal Accumulation Rates, Jenifer Bunty Aug 2015

Estimating Tiger (Panthera Tigris) Prey Density Using Camera Traps And Fecal Accumulation Rates, Jenifer Bunty

All Theses

The conservation of tiger populations requires the preservation of their prey. Assessing prey populations is therefore important, but challenges arise due to the elusive nature of many prey species. We used two indirect methods to estimate the density of sika deer (Cervus nippon), an elusive tiger prey species, in the Sikhote-Alin Biosphere Reserve in the Russian Far East. The fecal accumulation rate (FAR) is widely used and provides estimates of ungulate density based on the accumulation of fecal pellet groups in previously cleared plots. More recently, the random encounter model (REM) was developed to estimate population density from …


Interkingdom Communication: Study Of Caenorhabditis Elegans And Vibrio Cholerae Interactions, Joseph Angeloni Aug 2015

Interkingdom Communication: Study Of Caenorhabditis Elegans And Vibrio Cholerae Interactions, Joseph Angeloni

All Theses

Vibrio cholerae is a Gram-negative, rod-shaped bacterium that is mainly found in water environments, and is the causative agent of the disease cholera in humans. It is critical for this bacterium to communicate via quorum sensing to persist and survive in the environment, as well as cause infection. Recently, it has been shown that eukaryotes are able to sense and respond to certain quorum sensing molecules, known as autoinducers, which are produced by different bacteria. Caenorhabditis elegans is a particularly useful model for studying this interaction. During preliminary experiments, it was noticed that C. elegans were strongly attracted to V. …


Assessing The Impacts Of Macroalgal Competition And Parrotfish Grazing On Coral Cover In The Middle Florida Keys, Kylie Smith Aug 2015

Assessing The Impacts Of Macroalgal Competition And Parrotfish Grazing On Coral Cover In The Middle Florida Keys, Kylie Smith

All Theses

The coral reefs of the Florida Keys National Marine Sanctuary (FKNMS) are diverse ecosystems that support thousands of marine organisms. Unfortunately, the live coral cover on these reefs has dramatically decreased over the last 30 years even in the most protected areas of the sanctuary. Environmental changes have contributed to this decline in coral cover by creating optimal conditions for macroalgal growth. These macroalgae species are fast growing and out-compete slow growing corals for space and nutrients. Herbivores known to forage on macroalgae, including long-spined sea urchins and parrotfishes, have decreased in abundance. A synthesis of data from across the …


Environmental Effects On Fish Escape Responses: Impact Of Flow On The Escape Performance Of The Hawaiian Stream Goby, Sicyopterus Stimpsoni, Kelly Diamond Aug 2015

Environmental Effects On Fish Escape Responses: Impact Of Flow On The Escape Performance Of The Hawaiian Stream Goby, Sicyopterus Stimpsoni, Kelly Diamond

All Theses

The ability of fish to escape from predators is a critical behavior for their survival. Experimental measurements of escape performance in fishes have typically been based on trials conducted in still water; however, such environmental conditions are rare in nature due to waves and currents that expose fishes to unsteady and/or directional flow. I examined the effects of water flow and predator attack direction on the escape behavior of fish, using juveniles of the amphidromous Hawaiian goby Sicyopterus stimpsoni as a model system. In nature, these fish must escape ambush predation by the Hawaiian sleeper, Eleotris sandwicensis, while exposed …


Development, Validation, And Application Of Analytical Methods For Characterizing Adsorbed Protein Orientation, Conformation, And Bioactivity, Aby Thyparambil May 2015

Development, Validation, And Application Of Analytical Methods For Characterizing Adsorbed Protein Orientation, Conformation, And Bioactivity, Aby Thyparambil

All Dissertations

The structure and bioactivity of adsorbed proteins are tightly interrelated and play a key role in their interaction with the surrounding environment. These factors are of critical importance in many biotechnological applications. However, because the bioactive state of an adsorbed protein is a function of the orientation, conformation, and accessibility of its bioactive site(s), the isolated determination of just one or two of these factors will typically not be sufficient to understand the structure-function relationships of the adsorbed layer. Rather a combination of methods is needed to address each of these factors in a synergistic manner to provide a complementary …


Biomechanical Characterization And Modeling Of Human Tmj Disc, Gregory Wright May 2015

Biomechanical Characterization And Modeling Of Human Tmj Disc, Gregory Wright

All Dissertations

Temporomandibular joint (TMJ) disorder affects over 10 million people in the US each year. The signs and symptoms of temporomandibular joint disorders (TMDs) include limited mouth opening, clicking and locking of the jaw, and significant pain in the craniofacial region. In patients who seek treatment for TMDs, over 70% have TMJ disc displacement related to disc degeneration. The TMJ disc is interposed between the mandible condyle and the glenoid fossa of the temporal bone. The disc reduces contact stresses within the joint and provides lubrication to the joint. It is generally believed that pathological mechanical loadings, such as sustained jaw …


Life History And Natural Enemies Of Parthenolecanium Spp. In Four Southeastern States, Ernesto Robayo Camacho May 2015

Life History And Natural Enemies Of Parthenolecanium Spp. In Four Southeastern States, Ernesto Robayo Camacho

All Dissertations

Soft scales (Hemiptera: Coccidae) are some of the most prevalent and difficult to control arthropod pests in urban landscapes of the southern United States. European fruit lecanium, Parthenolecanium corni (Bouche), and oak lecanium, Parthenolecanium quercifex (Fitch), are commonly found in mixed populations as pests of oak trees (Quercus spp.) in the region. The phenology and natural enemies of the two species are poorly understood, which in turn hinders pest managers' ability to develop an integrated management program against these species. This research project aims to fill the information gaps concerning the life history and natural enemies of P. corni and …


Establishing A Permanent Feeding Site: Reniform Nematode Manipulation Of Soybean Genes, Nathan Wayne Redding May 2015

Establishing A Permanent Feeding Site: Reniform Nematode Manipulation Of Soybean Genes, Nathan Wayne Redding

All Theses

Reniform nematode, Rotelynchulus reniformis, is a semi-endoparasite capable of infecting more that 300 host plant species in tropical, subtropical, and warm temperate regions. Female nematodes penetrate host roots and introduce effectors that lead to the formation of multinucleate feeding sites called syncytia. The objective of our study was to identify plant genes involved in the process of syncitium formation. We used a split-root system in which half of a plant’s roots were inoculated with R. reniformis while the rest of the root system remained uninfected. Illumina RNA-seq was used to quantify gene expression patterns in replicate samples of infected and …


Chemical Separation And Analyses Of Bioactive Compounds In Chloroform Fraction Of Oplopanax Horridus (Devil's Club), Lauren Wiseman May 2015

Chemical Separation And Analyses Of Bioactive Compounds In Chloroform Fraction Of Oplopanax Horridus (Devil's Club), Lauren Wiseman

All Theses

O. horridus (a member of the family Araliaceae) is a plant traditionally used by the tribe of Pacific Northwest America for medicinal as well as spiritual purposes. O. horridus is found in the temperate forests of northwestern North America as an understory shrub capable of growing in well-drained to poorly drained, shady sites. Traditional medical preparations of O. horridus include decoction or infusion, preparation as a tea, preparation as a poultice, or preparation as a tonic. O. horridus is used as a form of treatment for cancer, hypoglycemia, diabetes, tough pneumonia, and for colds. A practical and reliable extraction method …


Assessing The Vulnerability Of Coastal Marsh Birds To Sea Level Rise In The South Atlantic Coast, Nicolette Roach May 2015

Assessing The Vulnerability Of Coastal Marsh Birds To Sea Level Rise In The South Atlantic Coast, Nicolette Roach

All Theses

Global wetland degradation and loss is occurring at a rapid rate, and in the United States over 50% of wetlands in the lower 48 states have been altered since European settlement. In some cases, wetlands that were historically transformed for agriculture are now managed as wetland habitat. We conducted occupancy surveys for black rails (Latterallus jamaicensis) in managed and unmanaged areas of coastal South Carolina. We modeled landscape and local factors potentially influencing occupancy and we assessed whether these habitat associations indicated vulnerability following expected alterations from sea level rise. Black rails occupied 17 of 344 sites surveyed. Landscape factors …


Variability In Movement Patterns And Habitat Use Of Two Species Of Pelecaniformes, Caroline Poli May 2015

Variability In Movement Patterns And Habitat Use Of Two Species Of Pelecaniformes, Caroline Poli

All Theses

Information regarding movement patterns of seabirds is useful for understanding prey abundance and distribution, predicting overlap between bird activity and anthropogenic stressors, and assessing the effectiveness of reserves at protecting nesting or foraging habitat(Cairns 1992, Weimerskirch 2007, Weimerskirch et al. 2010). I examined movement patterns of 40 brown pelicans (Pelecanus occidentalis) satellite tagged in December 2010 in South Carolina, USA, and tracked until July 2013. Birds exhibited individual variability in movement patterns and 25% of individuals remained near the breeding colony during nonbreeding. Birds captured in South Carolina that did migrate wintered as far south as Guatemala, prospected and used …


Finding The Needle And The Haystack: New Insights Into Locating Bog Turtles (Glyptemys Muhlenbergii) And Their Habitat In The Southeastern United States, Theresa Sonja Margareta Stratmann May 2015

Finding The Needle And The Haystack: New Insights Into Locating Bog Turtles (Glyptemys Muhlenbergii) And Their Habitat In The Southeastern United States, Theresa Sonja Margareta Stratmann

All Theses

Because rare and cryptic species can be difficult to locate, distribution maps for these species are often inaccurate or incomplete. Bog Turtles (Glyptemys muhlenbergii) are emblematic of this challenge. In fact, conducting surveys of known, historical, and potential Bog Turtle habitat is a specific need stated in the Bog Turtle Northern Population Recovery Plan and in most Comprehensive Wildlife Conservation Strategies of states within the southern range. Therefore we examined ways to better locate Bog Turtle habitat and Bog Turtles within that habitat. First we determined a detection probability for a standardized trapping method so we could effectively survey for …