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Full-Text Articles in Life Sciences
Application Of Data Analysis In Cold Stress: A Case Study Of Nicotiana Benthamiana, İbrahi̇m Koç, Zi̇hni̇ Onur Çalişkaner, Ertuğrul Fi̇li̇z
Application Of Data Analysis In Cold Stress: A Case Study Of Nicotiana Benthamiana, İbrahi̇m Koç, Zi̇hni̇ Onur Çalişkaner, Ertuğrul Fi̇li̇z
Turkish Journal of Botany
Cold stress is a major environmental factor in plant life cycles. Nicotiana benthamiana, which belongs to the family Solanaceae, is one of the most commonly used model species in plant-microbe interaction studies. In total, 5205 differentially expressed genes were identified under cold stress in N. Benthamiana. Of these, 5029 were upregulated and 176 were downregulated within four time periods (4 h, 12 h, 24 h, and 48 h). The common up- and downregulated genes were identified as 692 and 6, respectively. The functional annotations of these genes were studied and these common genes involved in protein, RNA, miscellaneous enzyme families, …
Effects Of Cobalt Nanoparticles On Artemisinin Production And Gene Expression In Artemisia Annua, Bita Ghasemi, Ramin Hosseini, Fatemeh Dehghan Nayeri
Effects Of Cobalt Nanoparticles On Artemisinin Production And Gene Expression In Artemisia Annua, Bita Ghasemi, Ramin Hosseini, Fatemeh Dehghan Nayeri
Turkish Journal of Botany
The low artemisinin content in Artemisia has caused this compound to be expensive among medicines. Several attempts have been made to increase its production by altering the expression of different genes. However, no approach has been cost-effective. In this study, the expression levels of SQS and DBR2 genes were quantified by qRT-PCR, and artemisinin content was measured by HPLC in Artemisia annua cell suspension culture under nano cobalt particles elicitation. For this purpose, nano cobalt particles were used in 0.25, 2.5, and 5 mg L-1 concentrations and samples were collected after 8, 24, 48, and 72 h. The highest artemisinin …