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Open Access. Powered by Scholars. Published by Universities.®

2015

Western Kentucky University

Ecology and Evolutionary Biology

Population genetics

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Phylogeography Of Darlingtonea Kentuckensis And Molecular Systematics Of Kentucky Cave Trechines, Olivia Frances Boyd May 2015

Phylogeography Of Darlingtonea Kentuckensis And Molecular Systematics Of Kentucky Cave Trechines, Olivia Frances Boyd

Masters Theses & Specialist Projects

The monotypic cave carabid genus Darlingtonea is widely distributed along the eastern band of the Mississippian/Pennyroyal plateau in Kentucky and northern Tennessee. DNA sequence data from the mitochondrial gene cytochrome oxidase subunit I (COI) was collected from one to four individuals from 27 populations, and patterns of phylogeography and population structure were inferred from COI haplotypes. A hierarchical analysis of molecular variance found low nucleotide diversity within populations and statistically significant variation among geographically-defined groups tested based on two a priori hypotheses of structure. Population structure among five distinct genetic clusters identifies approximate locations of barriers to gene flow among …