Life Sciences Commons^™

Response Of Soil Nitrification To The Veterinary Pharmaceuticals Monensin, Ivermectin And Zinc Bacitracin, Magda A. Konopka

Electronic Thesis and Dissertation Repository

Pharmaceutical residues can reach agricultural land through amendment with animal or human waste. Since 2010, a series of replicated plots received annual applications of ivermectin, monensin and zinc bacitracin, either singly or in a mixture, at 0.1 mg/kg and 10 mg/kg concentrations. I collected soil samples before and after the fourth annual application of pharmaceuticals and assayed them for functional changes and amoA gene abundance, a gene needed for ammonia oxidation. In 2013, I exposed the soils to 100 mg/kg in a laboratory experiment which resulted in acceleration of nitrification. Under 10 mg/kg treatments in the field the abundance of …

Fragipan Horizon Fragmentation In Slaking Experiments With Amendment Materials And Ryegrass Root Tissue Extracts, Anastasios D. Karathanasis, Lloyd W. Murdock, Christopher J. Matocha, John H. Grove, Yvonne L. Thompson

Plant and Soil Sciences Faculty Publications

Slaking experiments were conducted of fragipan clods immersed in solutions of poultry manure, aerobically digested biosolid waste (ADB), fluidized bed combustion byproduct (FBC), D-H₂O, CaCO₃, NaF, Na-hexa-metaphosphate, and ryegrass root biomass. The fragipan clods were sampled from the Btx horizon of an Oxyaquic Fragiudalf in Kentucky. Wet sieving aggregate analysis showed significantly better fragmentation in the NaF, Na-hexa-metaphosphate, and ryegrass root solutions with a mean weight diameter range of 15.5-18.8 mm compared to the 44.2-47.9 mm of the poultry manure, ADB, and FBC treatments. Dissolved Si, Al, Fe, and Mn levels released in solution were ambiguous. …

Association Of Soil Conditions And Grass Species With Variable Cover Of Leafy Spurge, Terence P. Mcgonigle, Jeremy L. Timmer

The Prairie Naturalist

Variation in soil conditions and grass cover was assessed across a range of leafy spurge (Euphorbia esula L.) cover values on a sandy rangeland in Manitoba, Canada. Soil conditions varied by site but not in relation to cover of leafy spurge. We observed a significant negative relationship between total grass cover and increasing cover of leafy spurge. Only porcupine grass (Hesperostipa spartea [Trin.] Barkworth) had a negative relationship with leafy spurge, falling from high cover at low weed occurrence to only trace levels at the highest leafy spurge abundance. Neither prairie Junegrass (Koeleria macrantha [Ledeb.] Schult.), rough …

Microbial Diversity Of A Mediterranean Soil And Its Changes After Biotransformed Dry Olive Residue Amendment, José A. Siles, Caio T.C.C Rachid, Inmaculada Sampedro, Inmaculada García-Romera, James M. Tiedje

Dartmouth Scholarship

The Mediterranean basin has been identified as a biodiversity hotspot, about whose soil microbial diversity little is known. Intensive land use and aggressive management practices are degrading the soil, with a consequent loss of fertility. The use of organic amendments such as dry olive residue (DOR), a waste produced by a two-phase olive-oil extraction system, has been proposed as an effective way to improve soil properties. However, before its application to soil, DOR needs a pre-treatment, such as by a ligninolytic fungal transformation, e.g. Coriolopsis floccosa. The present study aimed to describe the bacterial and fungal diversity in a …

Ecological Biogeography Of The Terrestrial Nematodes Of Victoria Land, Antarctica, Byron Adams, Diana Wall, Ross Virginia, Emma Broos, Matthew A. Knox

Dartmouth Scholarship

The terrestrial ecosystems of Victoria Land, Antarctica are characteristically simple in terms of biological diversity and ecological functioning. Nematodes are the most commonly encountered and abundant metazoans of Victoria Land soils, yet little is known of their diversity and distribution. Herein we present a summary of the geographic distribution, habitats and ecology of the terrestrial nematodes of Victoria Land from published and unpublished sources. All Victoria Land nematodes are endemic to Antarctica, and many are common and widely distributed at landscape scales. However, at smaller spatial scales, populations can have patchy distributions, with the presence or absence of each species …

Nitrogen Inputs And Losses In Response To Chronic Co2 Exposure In A Subtropical Oak Woodland, B. A. Hungate, B. D. Duval, P. Dijkstra, D. W. Johnson, M. E. Ketterer, Peter Stiling, W. Cheng, J. Millman, A. Hartley, D. B. Stover

Integrative Biology Faculty and Staff Publications

Rising atmospheric CO₂ concentrations may alter the nitrogen (N) content of ecosystems by changing N inputs and N losses, but responses vary in field experiments, possibly because multiple mechanisms are at play. We measured N fixation and N losses in a subtropical oak woodland exposed to 11 years of elevated atmospheric CO₂ concentrations. We also explored the role of herbivory, carbon limitation, and competition for light or nutrients in shaping the response of N fixation to elevated CO₂. Elevated CO₂ did not significantly alter gaseous N losses, but lower recovery and deeper distribution in the …

The Effects Of Soil Ph And Texture On The Molting Success And Survival Of Blacklegged Ticks (Ixodes Scapularis): A Field Experiment, Colleen Cook

Honors Theses

The blacklegged tick (Ixodes scapularis) is the primary vector of Borrelia burgdorferi, the causative agent of Lyme disease. When a tick is not questing or feeding, the majority of its life is spent within the soil. Abiotic factors within soil have been shown to affect tick molting and survival across all life stages. Soil pH, however, has not been heavily investigated. In this field study, I investigated the effects of soil pH and texture on engorged nymphal ticks. Two sites were chosen to encompass the extremes of soil pH in the region; the Albany Pine Bush in Albany, NY has …

The Effects Of Soil Ph On The Molting Success Of Blacklegged Ticks (Ixodes Scapularis): A Laboratory Experiment, Daniel Rice

Honors Theses

Ixodes scapularis, or the black-legged tick, is the major vector of Lyme disease in the U.S. I. scapularis has expanded its range in recent decades, making the study of factors affecting its distribution a high priority. Studying the effects of various conditions in the soil could help in predicting range expansions, because ticks spend the majority of their lives in contact with the soil. We investigated the effects of soil pH on the molting success of engorged I. scapularis nymphs collected from Eastern Chipmunks (Tamias striatus). The experiment was conducted in a laboratory to control for covariates such as temperature …

Spreading The Char: The Importance Of Local Compatibility In The Diffusion Of Biochar Systems To The Smallholder Agriculture Community Context, Laura C. V. Munoz

Pomona Senior Theses

This thesis enters the context of smallholder agriculture communities in the developing world. It explores the potentials of biochar and what biochar systems could bring to the smallholder communities while simultaneously bringing environmental benefits. It then acknowledges the challenges of diffusion –the spreading of an unfamiliar innovation. It seeks to answer the question of what will make diffusion of biochar systems more successful in the smallholder context, fixating on the characteristic of compatibility as well as the role local community members can play in making a new biochar system more visible to the rest of the communities.

Induced Transcriptional Profiling Of Phenylpropanoid Pathway Genes Increased Flavonoid And Lignin Content In Arabidopsis Leaves In Response To Microbial Products, Mohammad Babar Ali, David H. Mcnear

Plant and Soil Sciences Faculty Publications

BACKGROUND: The production and use of biologically derived soil additives is one of the fastest growing sectors of the fertilizer industry. These products have been shown to improve crop yields while at the same time reducing fertilizer inputs to and nutrient loss from cropland. The mechanisms driving the changes in primary productivity and soil processes are poorly understood and little is known about changes in secondary productivity associated with the use of microbial products. Here we investigate secondary metabolic responses to a biologically derived soil additive by monitoring changes in the phenlypropanoid (PP) pathway in Arabidopsis thaliana.

RESULTS: This study …

Quantitative Field Testing Heterodera Glycines From Metagenomic Dna Samples Isolated Directly From Soil Under Agronomic Production., Yan Li, Gary W. Lawrence, Shien Lu, Clarissa Balbalian, Vincent P. Klink

College of Agriculture & Life Sciences Publications and Scholarship

A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.

Impacts Of Climate Change On Soil Microorganisms In Northern Hardwood Forests, Carley Jane Kratz

Dissertations, Master's Theses and Master's Reports - Open

As global climate continues to change, it becomes more important to understand possible feedbacks from soils to the climate system. This dissertation focuses on soil microbial community responses to climate change factors in northern hardwood forests. Two soil warming experiments at Harvard Forest in Massachusetts, and a climate change manipulation experiment with both elevated temperature and increased moisture inputs in Michigan were sampled. The hyphal in-growth bag method was to understand how soil fungal biomass and respiration respond to climate change factors. Our results from phospholipid fatty acid (PLFA) analyses suggest that the hyphal in-growth bag method allows relatively pure …

Methods For The Study Of Soil Charcoal As An Indicator Of Fire And Forest History In The Appalachian Region, U.S.A., Sally P Horn, Christopher A. Underwood

Geography Publications and Other Works

Charcoal particles in soils and sediments of the Appalachian region provide evidence of long-term fire history relevant to resource management and to studies of paleoclimate, vegetation history, and the effects of prehistoric and historic humans on the environment. Charcoal records of fire history are of low resolution in comparison to dendrochronological records, but reach well beyond the oldest trees in most areas, providing evidence of fires thousands or tens of thousands of years ago. We focus here on fire history reconstruction from soil charcoal, which provides site-specific evidence of past fires and potentially forest composition. Charcoal > 2 mm may be …

Tackling Soil Diversity With The Assembly Of Large, Complex Metagenomes, Adina Howe, Janet K. Jansson, Stephanie Malfatti, Susannah Tringe, James Tiedje, C. Titus Brown

Adina Howe

Investigations of complex environments rely on large volumes of sequence data to adequately sample the genetic diversity of a microbial community. The assembly of short-read data into longer, more interpretable sequence currently is not possible for much of the research community because it requires specialized computational facilities. We present approaches that make de novo assembly of complex metagenomes more accessible. These approaches scale data size with community richness and subdivide the data into tractable subsets representing individual species. We applied these methods toward the assembly of two large soil metagenomes to identify important metagenomic references and show that considerably more …

2013 Wild Blueberry Project Reports, Vivian Wu, Dorothy J. Klimis-Zacas, Frank A. Drummond, Judith A. Collins, Alex Bajcz, Lee Beers, Brianne Looze, Cyndy Loftin, Aaron Hoshide, Sara Bushmann, Kalyn Bickerman, Alison Dibble, Lois Berg Stack, Gabriel Al-Najjar, Elissa Ballman, Seanna L. Annis, Caleb Slemmons, David E. Yarborough, Jennifer L. D'Appollonio, Mary Ellen Camire, Jennifer R. Chadbourne, Michael Dougherty, Katherine Davis-Dentici, Edward Bernard, Tamara Levitsky, Tsutomu Ohno, Erika Lyon, Ellen Mallory, Katie Mcphee, Hannah Griffin, Marianne Sarrantonio

Wild Blueberry Research Reports

The 2013 edition of the Wild Blueberry Project Reports was prepared for the Wild Blueberry Commission of Maine and the Wild Blueberry Advisory Committee by researchers at the University of Maine, Orono. Projects in this report include:

1. Development of effective intervention measures to maintain and improve food safety for wild blueberries

2. Do wild blueberries alleviate risk factors related to the Metabolic Syndrome?

3. Wild Blueberry consumption and exercise-induced Oxidative Stress: Inflammatory Response and DNA damage

4. Control tactics for blueberry pest insects, 2013

5. Pesticide residues on wild blueberry, 2013

6. Biology of pest insects and IPM, 2013 …