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Full-Text Articles in Life Sciences
Cell-Type Specific Transcriptomic Profiling To Dissect Mechanisms Of Differential Dendritogenesis, Surajit Bhattacharya, Eswar Prasad R. Iyer, Srividya Chandramouli Iyer, Daniel N. Cox
Cell-Type Specific Transcriptomic Profiling To Dissect Mechanisms Of Differential Dendritogenesis, Surajit Bhattacharya, Eswar Prasad R. Iyer, Srividya Chandramouli Iyer, Daniel N. Cox
Neuroscience Institute Faculty Publications
The establishment, maintenance and modulation of cell-type specific neural architectures are critically important to the formation of functional neural networks. At the neuroanatomical level, differential patterns of dendritic arborization directly impact neural function and connectivity, however the molecular mechanisms underlying the specification of distinct dendrite morphologies remain incompletely understood. To address this question, we analyzed global gene expression from purified populations of wild-type class I and class IV Drosophila melanogaster dendritic arborization (da) sensory neurons compared to wild-type whole larval RNA using oligo DNA microarray expression profiling. Herein we present detailed experimental methods and bioinformatic anal- yses to correspond with …
Oligonucleotide Design For Whole Genome Tiling Arrays, Qin Dong
Oligonucleotide Design For Whole Genome Tiling Arrays, Qin Dong
Electronic Thesis and Dissertation Repository
Oligonucleotides are short, single-stranded fragments of DNA or RNA, designed to readily bind with a unique part in the target sequence. They have many important applications including PCR (polymerase chain reaction) amplification, microarrays, or FISH (fluorescence in situ hybridization) probes. While traditional microarrays are commonly used for measuring gene expression levels by probing for sequences of known and predicted genes, high-density, whole genome tiling arrays probe intensively for sequences that are known to exist in a contiguous region. Current programs for designing oligonucleotides for tiling arrays are not able to produce results that are close to optimal since they allow …