Life Sciences Commons^™

Functional Genomics And Microbiome Profiling Of The Asian Longhorned Beetle (Anoplophora Glabripennis) Reveal Insights Into The Digestive Physiology And Nutritional Ecology Of Wood Feeding Beetles, Erin D. Scully, Scott M. Geib, John E. Carlson, Ming Tien, Duane Mckenna, Kelli Hoover

Department of Agronomy and Horticulture: Faculty Publications

Background: Wood-feeding beetles harbor an ecologically rich and taxonomically diverse assemblage of gut microbes that appear to promote survival in woody tissue, which is devoid of nitrogen and essential nutrients. Nevertheless, the contributions of these apparent symbionts to digestive physiology and nutritional ecology remain uncharacterized in most beetle lineages.

Results: Through parallel transcriptome profiling of beetle- and microbial- derived mRNAs, we demonstrate that the midgut microbiome of the Asian longhorned beetle (Anoplophora glabripennis), a member of the beetle family Cerambycidae, is enriched in biosynthetic pathways for the synthesis of essential amino acids, vitamins, and sterols. Consequently, the midgut …

The Exometabolome Of Clostridium Thermocellum Reveals Overflow Metabolism At High Cellulose Loading, Evert K. Holwerda, Philip G. Thorne, Daniel G. Olson, Daniel Amador-Noguez, Nancy L. Engle, Timothy J. Tschaplinski, Johannes P. Van Dijken, Lee R. Lynd

Dartmouth Scholarship

BackgroundClostridium thermocellum is a model thermophilic organism for the production of biofuels from lignocellulosic substrates. The majority of publications studying the physiology of this organism use substrate concentrations of ≤10 g/L. However, industrially relevant concentrations of substrate start at 100 g/L carbohydrate, which corresponds to approximately 150 g/L solids. To gain insight into the physiology of fermentation of high substrate concentrations, we studied the growth on, and utilization of high concentrations of crystalline cellulose varying from 50 to 100 g/L by C. thermocellum. .

Regulation Of The Candida Albicans Arginine Biosynthetic Pathway, Claudia Jimenez Lopez

Dissertations & Theses (Open Access)

Candida albicans is the most importan thuman-associatedfungus.It is a commensal microorganism but also an opportunistic pathogen able to cause superficial infections aswellaslife-threateninginfectionswhich are associated with a highmortalityrateof 50%. The interactions between C. albicans and the cells of the mammalian innate immune system, which confer the most important protecting mechanisms against disseminated infections, are very dynamic and determine the success of C. albicans as a pathogen. Transcriptional profiling has shown that phagocytosis of C. albicans by macrophages results primarily in the activation of alternative carbon metabolism pathways suggesting that the pathogen is exposed to a glucose poor environment. Changes in …

Decrements Of Muscle Protein Synthesis With Unloading Are Not Due To Insufficient Concentrations Of Intramuscular Leucine, Shaik T. Ullah, Kevin Shimkus, Yasaman Shirazi-Fard, Camilo Pohlenz, Jacqueline Perticone, Del Gatlin, Harry Hogan, James Fluckey

International Journal of Exercise Science: Conference Proceedings

Skeletal muscle mass and strength play critical roles in quality of life, and significant muscle atrophy contributes to reduced function and can exacerbate some disease states. It is well-known that persistent reductions of mechanical loading in skeletal muscle result in degeneration. Generally, reductions of muscle protein synthesis are, at least in part, a major culprit with muscle loss under these conditions, and numerous countermeasures such as exercise and nutritional supplements, known to stimulate protein synthesis have been designed to maintain muscle mass under those conditions. Amino acid supplementation, particularly with branched-chain amino acids (BCAAs), has been suggested as a countermeasure …

The Effects Of Vespa Amino Acid Mixture On Cycling Performance During A 20k Time Trial, Sebastian Haynes, Adam Parker

International Journal of Exercise Science: Conference Proceedings

The effects of Vespa Amino Acid Mixture on cycling performance during a 20k time trial.

Sebastian Haynes and Adam Parker, Ph.D.

Department of Kinesiology

Angelo State University, San Angelo, TX

Category: Undergraduate

Mentor: Adam Parker (adam.parker@angelo.edu)

Abstract

Vespa amino acid mixture (VAAM) is a nutritional supplement derived from the Asian Mandarin Wasp (Vespa Mandarina). VAAM has been shown to enhance lipolysis in rat adipocytes and is purported to improve endurance performance via enhanced fat metabolism. The purpose of this study was to examine the effects VAAM on cycling performance during a 20k time trial. 10 trained, male cyclists …

L-Arginine Impact On Cherry Rootstock Rooting And Biochemical Characteristics In Tissue Culture, Virginia Sarropoulou, Kortessa Dimassi-Theriou, Ioannis Therios

Turkish Journal of Agriculture and Forestry

In the present study, the effects of indole-3-butyric acid (IBA) separately and simultaneously with L-arginine on the morphogenic and biochemical responses in the cherry rootstocks CAB-6P (Prunus cerasus L.) and Gisela 6 (Prunus cerasus × Prunus canescens) were investigated. In the CAB-6P rootstock, the best root number and root length results were obtained with 2 mg L^{–1} IBA plus 0.5 mg L^{–1} L-arginine and 1 mg L^{–1} IBA plus 1 mg L^{–1} L-arginine, respectively. The rooting percentage was highest (100%) with 2 mg L^{–1} IBA alone or combined with 1 mg L^{–1} L-arginine. In the Gisela 6 explants, 2 mg …

Dietary Supplement Labeling: Cognitive Biases, Market Manipulation & Consumer Choice, Michael Mccann

Law Faculty Scholarship

There exists increasing concern that the Dietary Supplements Health and Education Act (DSHEA) has proven ineffective. Much of the concern regards the disparity in legislative treatment between dietary supplements, foods, and pharmaceutical drugs. Namely, while pharmaceutical drugs must undergo years of costly pre-market testing, most supplements, like foods, can immediately enter the market, and only after repeated instances of adverse reactions can the Food and Drug Administration (FDA) remove them. Such a framework appears to belie both consumer expectations and marketing strategies, as supplements tend to be most perceived for their apparent medicinal qualities. This philosophy of waiting for a …

A Coupled Geochemical And Biogeochemical Approach To Characterize The Bioreactivity Of Dissolved Organic Matter From A Headwater Stream, Rachel L. Sleighter, Rose M. Cory, Louis A. Kaplan, Hussain A.N. Abdulla, Patrick G. Hatcher

Chemistry & Biochemistry Faculty Publications

The bioreactivity or susceptibility of dissolved organic matter (DOM) to microbial degradation in streams and rivers is of critical importance to global change studies, but a comprehensive understanding of DOM bioreactivity has been elusive due, in part, to the stunningly diverse assemblages of organic molecules within DOM. We approach this problem by employing a range of techniques to characterize DOM as it flows through biofilm reactors: dissolved organic carbon (DOC) concentrations, excitation emission matrix spectroscopy (EEMs), and ultrahigh resolution mass spectrometry. The EEMs and mass spectral data were analyzed using a combination of multivariate statistical approaches. We found that 45% …