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Full-Text Articles in Life Sciences

Changes In Tissue-Specific Fatty Acid Composition Of The Freshwater Alewife (Alosa Pseudoharengus) In Response To Temperature, William D. Schregel Dec 2013

Changes In Tissue-Specific Fatty Acid Composition Of The Freshwater Alewife (Alosa Pseudoharengus) In Response To Temperature, William D. Schregel

Biology Theses

Ectotherms are known to alter the composition of the cell membrane in response to changes in environmental temperature. Tissue-specific fatty acid composition in the alewife (Alosa pseudoharengus) was examined in response to both warm and cold temperature challenges administered over an approximate one month period. Gill, muscle, and liver tissues were analyzed prior to the start of the temperature challenge (initial), following the temperature challenges (survivors), and on those fish that did not survive the temperature challenges (mortalities). Significant differences were found between fatty acid composition of initial fish and survivors for membrane-incorporated fatty acids (polar) and for …


Differences In Temperature Responses Of Achene Types In Centaurea Melitensis, Kandee L. Bain Nov 2013

Differences In Temperature Responses Of Achene Types In Centaurea Melitensis, Kandee L. Bain

LUX: A Journal of Transdisciplinary Writing and Research from Claremont Graduate University

Southern California has a Mediterranean climate with wet winters and hot dry summers. This type of environment is associated with irregular temperature patterns and rainfall, which can be unpredictable. Unpredictable environments, can threaten the germination and survival of plants like Centaurea melitensis. To ensure germination and survival under unpredictable environmental conditions, some plants may develop characteristic structures like cleistogamous flower heads, and in some cases they will develop heteromorphic achenes. Morphological differences between peripheral and center achenes may indicate variable responses to temperature. Additionally, unpredictable environmental conditions may lead to differences in the number of peripheral to center achenes …


The Rootscope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots, Erin J. Kast , '15, Minh-Duyen T. Nguyen , '13, Rosalie E. Lawrence , '12, C. Rabeler, Nicholas J. Kaplinsky Oct 2013

The Rootscope: A Simple High-Throughput Screening System For Quantitating Gene Expression Dynamics In Plant Roots, Erin J. Kast , '15, Minh-Duyen T. Nguyen , '13, Rosalie E. Lawrence , '12, C. Rabeler, Nicholas J. Kaplinsky

Biology Faculty Works

Background: High temperature stress responses are vital for plant survival. The mechanisms that plants use to sense high temperatures are only partially understood and involve multiple sensing and signaling pathways. Here we describe the development of the RootScope, an automated microscopy system for quantitating heat shock responses in plant roots.Results: The promoter of Hsp17.6 was used to build a Hsp17.6(p):GFP transcriptional reporter that is induced by heat shock in Arabidopsis. An automated fluorescence microscopy system which enables multiple roots to be imaged in rapid succession was used to quantitate Hsp17.6p: GFP response dynamics. Hsp17.6(p):GFP signal increased with temperature increases from …


The Effects Of Extrinsic And Intrinsic Factors On The Reproductive Biology Of Male Western Bean Cutworm Moths, Striacosta Albicosta (Smith), Jasmine Farhan Aug 2013

The Effects Of Extrinsic And Intrinsic Factors On The Reproductive Biology Of Male Western Bean Cutworm Moths, Striacosta Albicosta (Smith), Jasmine Farhan

Electronic Thesis and Dissertation Repository

Pheromones are important in the mating systems of nocturnal moths as they are relied on to find and/or assess mates. Extrinsic and intrinsic factors have been shown to influence female emission of and male response to pheromones. My thesis focuses on the reproductive biology of males of the western bean cutworm, Striacosta albicosta, a pest of beans and corn that has recently began expanding its range eastwardly. I conducted a field-based experiment to determine the effects of extrinsic factors on pheromone trap catches. I also conducted laboratory based mating experiments to determine the effect of male age on acceptance …


Subset Of Heat-Shock Transcription Factors Required For The Early Response Of Arabidopsis To Excess Light, Hou-Sung Jung, Peter A. Crisp, Gonzalo M. Estavillo, Benjamin Cole Aug 2013

Subset Of Heat-Shock Transcription Factors Required For The Early Response Of Arabidopsis To Excess Light, Hou-Sung Jung, Peter A. Crisp, Gonzalo M. Estavillo, Benjamin Cole

Dartmouth Scholarship

Sunlight provides energy for photosynthesis and is essential for nearly all life on earth. However, too much or too little light or rapidly fluctuating light conditions cause stress to plants. Rapid changes in the amount of light are perceived as a change in the reduced/oxidized (redox) state of photosynthetic electron transport components in chloroplasts. However, how this generates a signal that is relayed to changes in nuclear gene expression is not well understood. We modified redox state in the reference plant, Arabidopsis thaliana, using either excess light or low light plus the herbicide DBMIB (2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone), a well-known inhibitor of photosynthetic …


The Effect Of Temperature Extremes On Cardiorespiratory Function In The Grass Shrimp Palaemonetes Pugio: Oxygen Limited Thermal Tolerance, Teresa Mika Aug 2013

The Effect Of Temperature Extremes On Cardiorespiratory Function In The Grass Shrimp Palaemonetes Pugio: Oxygen Limited Thermal Tolerance, Teresa Mika

UNLV Theses, Dissertations, Professional Papers, and Capstones

Aquatic poikilothermic animals must either cope with or compensate for the mismatch in oxygen supply and demand present at high temperatures. Oxygen limited thermal tolerance explains how aerobic scope is limited by insufficient oxygen supply and sets the performance window in animals. This work explores the effects of temperature on the different components of the oxygen delivery system, in both normoxic and hyperoxic conditions to determine if supplying more oxygen to the system mitigates the effects of temperature effects on the cardiorespiratory system and extends oxygen limited thermal tolerance to higher temperatures. The effect of temperature and oxygen condition was …


Regulation Of Succinate-Fuelled Mitochondrial Respiration In Liver And Skeletal Muscle Of Hibernating Thirteen-Lined Ground Squirrels., Jason C L Brown, Dillon J Chung, Alex N Cooper, James F Staples May 2013

Regulation Of Succinate-Fuelled Mitochondrial Respiration In Liver And Skeletal Muscle Of Hibernating Thirteen-Lined Ground Squirrels., Jason C L Brown, Dillon J Chung, Alex N Cooper, James F Staples

Biology Publications

Hibernating ground squirrels (Ictidomys tridecemlineatus) alternate between two distinct metabolic states throughout winter: torpor, during which metabolic rate (MR) and body temperature (Tb) are considerably suppressed, and interbout euthermia (IBE), during which MR and Tb briefly return to euthermic levels. Previous studies showed suppression of succinate-fuelled respiration during torpor in liver and skeletal muscle mitochondria; however, these studies used only a single, saturating succinate concentration. Therefore, they could not address whether mitochondrial metabolic suppression occurs under physiological substrate concentrations or whether differences in the kinetics of mitochondrial responses to changing substrate concentration might also contribute to mitochondrial metabolic regulation during …


Are Incomplete Denitrification Pathways A Common Trait In Thermus Species From Geothermal Springs In China?, Julienne J. Paraiso, Brian P. Hedlund Jan 2013

Are Incomplete Denitrification Pathways A Common Trait In Thermus Species From Geothermal Springs In China?, Julienne J. Paraiso, Brian P. Hedlund

McNair Poster Presentations

Temperature has strong impacts on ecosystem function and biogeochemical cycles, particularly within extreme environments such as geothermal springs above 60 °C. The primary focus of this study was to investigate the denitrification pathways of Thermus (Bacteria) isolates from geothermal springs from Tengchong, China. This study tested the hypothesis that incomplete denitrification is a common characteristic of the genus Thermus, regardless of geographic origin or species affiliation, which would implicate them in the efflux of nitrous oxide (a strong greenhouse gas) to the atmosphere. In this study, we cultivated 25 isolates, including six known Thermus species, and measured the stoichiometry …