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Full-Text Articles in Life Sciences

Geoexes - My Dos Executables - Use At Your Own Risk, George Mcnamara Dec 2013

Geoexes - My Dos Executables - Use At Your Own Risk, George Mcnamara

George McNamara

The downloadable ZIP file contains old Turbo Pascal programs I wrote. These ran in Microscoft DOS and in early Windows (ex. Windows 3.1) DOS shells. They may or may not work in recent (Windows 2000, XP, Vista, 7) cmd or command "shells". No warranty. Use at your own risk. I do not expect to ever program in Turbo Pascal again so if anyone wants to "take over" my old code (and adjust copyirght notices, warnings, etc), please email me. In particular, MATH.exe had 10,000 lines of pascal code in it. Another program is LUTSconv.exe which converted Fractint MAP lookup tables …


Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara Nov 2013

Stellaris Fishing 20131125mon Part 1 Of 2, George Mcnamara

George McNamara

Stellaris FISH dataset using three FISH probe sets. Slides courtesy of Biosearch Technologies,

https://www.biosearchtech.com/store/product.aspx?catid=224,318,324

see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.

This experiment was to evaluate the crosstalk between the Biosearch fluorophores:

Quasar 570

CAL Fluor Red 610 (CFR 610)

Quasar 670

DAPI (DNA counterstain)

Autofluorescence (green, but sometimes showing up in other channels).

and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:

DAPI

GFP (L5)

Cy3 (N3)

Texas Red (TxRed2)

Cy5 (Y5)

I also acquired green channel and red channel with exciter filters in our ASI excitation wheel:

GFP + 492 exciter

Texas Red (TxRed2) + 572 …


Stellaris Fishing 20131125mon Part 2 Of 2, George Mcnamara Nov 2013

Stellaris Fishing 20131125mon Part 2 Of 2, George Mcnamara

George McNamara

Stellaris FISH dataset using three FISH probe sets.

Slides courtesy of Biosearch Technologies,

https://www.biosearchtech.com/store/product.aspx?catid=224,318,324

see http://stellarisfish.smugmug.com/ for online gallery by Biosearch.

This experiment was to evaluate the crosstalk between the Biosearch fluorophores:

Quasar 570

CAL Fluor Red 610 (CFR 610)

Quasar 670

DAPI (DNA counterstain)

Autofluorescence (green, but sometimes showing up in other channels).

and our lab's Leica DMI6000 fluorescence microscope with Leica filter sets:

DAPI

GFP (L5)

Cy3 (N3)

Texas Red (TxRed2)

Cy5 (Y5)

I also acquired green channel and red channel with exciter filters in our ASI excitation wheel:

GFP + 492 exciter

Texas Red (TxRed2) + 572 …


Goby Climbing Selection, Dominica Versus Hawaii, Sandy M. Kawano, William C. Bridges, Heiko L. Schoenfuss, Takashi Maie, Richard W. Blob Sep 2013

Goby Climbing Selection, Dominica Versus Hawaii, Sandy M. Kawano, William C. Bridges, Heiko L. Schoenfuss, Takashi Maie, Richard W. Blob

All Data Sets

Data set containing morphology data that was used for the selection analyses in the article "Differences in locomotor behavior correspond to different patterns of morphological selection in two species of waterfall-climbing gobiid fishes" Evolutionary Ecology, September 2013, Volume 27, Issue 5, pp 949-969.


Shape Changes In Hyoid Arch Elements In Four Shark Species, Isabel S. Nowinowski May 2013

Shape Changes In Hyoid Arch Elements In Four Shark Species, Isabel S. Nowinowski

Senior Honors Projects

The hyoid arch is critical in expanding the oral cavity during feeding and ventilation in fishes. The elements of the hyoid arch vary widely in morphology among fish taxa. The hyoid arch is medial to the jaws and is made up of paired hyomandibular cartilages (HY) dorsally, paired ceratohyal cartilages (CH) ventral to the HY, and a single, medial basihyal cartilage (BH) that interconnects the two sides. As the jaws open, the CH-BH complex is depressed expanding the floor of the oral cavity. The coracohyoideus muscle pulls the BH ventroposteriorly, which in turn swings the CH ventroposteriorly. As the coracohyoideus …


Flash4 Dark Reference Images, George Mcnamara Apr 2013

Flash4 Dark Reference Images, George Mcnamara

George McNamara

Hamamatsu FLASH4.0 dark reference images, acquired with 10 second exposure times, no light to camera. Camera offset (set by Hamamatsu( is ~100 (the average intensity of the first image is always ~1 intensity level higher - an odd feature, but trivial in practice for a 16-bit camera).

George McNamara, Ph.D.

Single Cells Analyst at L.J.N. Cooper Lab

University of Texas M.D. Anderson Cancer Center


Pubspectra Tattletales, George Mcnamara Feb 2013

Pubspectra Tattletales, George Mcnamara

George McNamara

Tattletales for Multiplex Fluorescent Reporters in Single Cells for Metabolomics

George McNamara

As of April 2013: L.J.N. Cooper & D.A. Lee Cellular Immunotherapy Lab, University of Texas M.D. Anderson Cancer Center, Houston, TX

Email: gtmcnamara@mdanderson.org, geomcnamara@earthlink.net

Tattletales is my concept for spatial multiplexing many fluorescent protein (FP) biosensors in the same live cell. For example, there are excellent FP biosensors to Ca++ ions, pH, glucose, ribose, glutamine, glutamate, ATP, redox, ROS, pyruvate, cAMP, cGMP, IP3, PI(3,4,5)P3, cell cycle indicators (Fucci2), PKA, PKC, photsphatases, caspase(s) [1, 2]. However, these are typically used one biosensor per experiment, due in part to flooding …


2013 Ii Cu Suri (Common Poster), Vanessa Selimovic, Loriann Reese, Ruthie Nelson, Emma Sleeman, Hibery Ho, Maria Polozova Jan 2013

2013 Ii Cu Suri (Common Poster), Vanessa Selimovic, Loriann Reese, Ruthie Nelson, Emma Sleeman, Hibery Ho, Maria Polozova

CUP Undergraduate Research

A common poster providing information for several research projects: aerosol particles, deoxycytidine kinase, squaraine rotaxanes, glycol nucleic acied, and Kombucha.


Life History, C. W. Fox, Frank J. Messina Jan 2013

Life History, C. W. Fox, Frank J. Messina

Biology Faculty Publications

No abstract provided.


An Allele Of Sequoia Dominantly Enhances A Trio Mutant Phenotype To Influence Drosophila Larval Behavior, Kathryn E. Dean, April Fields, Marcus J. Geer, Eric C. King, Eric C. Liebl Jan 2013

An Allele Of Sequoia Dominantly Enhances A Trio Mutant Phenotype To Influence Drosophila Larval Behavior, Kathryn E. Dean, April Fields, Marcus J. Geer, Eric C. King, Eric C. Liebl

Faculty Publications

No abstract provided.