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Articles 1 - 6 of 6
Full-Text Articles in Life Sciences
Localization And Mutational Analysis Of The Nuclear And Aggregation-Prone Ime4 Protein In Saccharomyces Cerevisiae, Patricia M. Dehon
Localization And Mutational Analysis Of The Nuclear And Aggregation-Prone Ime4 Protein In Saccharomyces Cerevisiae, Patricia M. Dehon
University of New Orleans Theses and Dissertations
In Saccharomyces cerevisiae, Ime4 is a protein that is induced during meiosis and has a primary role in regulating sporulation in starving diploids. One function of Ime4 is methylation of adenosine residues within mRNA transcripts. Recent studies have shown Ime4 to be induced in haploids during the mating response, although its role in mating has not been determined. In this report, I identify the subcellular localization of Ime4 during the mating response through treatment with alpha factor. A plasmid containing IME4-GFP under the control of the medium strength promoter CYC1 was created in order to express the protein in a …
Characterization Of The Monomer-Dimer Equilibrium Of Recombinant Histo-Aspartic Protease From Plasmodium Falciparum, Huogen Xiao, Lee-Ann Briere, Stanley Dunn, Rickey Yada
Characterization Of The Monomer-Dimer Equilibrium Of Recombinant Histo-Aspartic Protease From Plasmodium Falciparum, Huogen Xiao, Lee-Ann Briere, Stanley Dunn, Rickey Yada
Stanley D Dunn
Histo-aspartic protease (HAP) from Plasmodium falciparum is an intriguing aspartic protease due to its unique structure. Our previous study reported the first recombinant expression of soluble HAP, in its truncated form (lys77p-Leu328) (p denotes prosegment), as a thioredoxin (Trx) fusion protein Trx-tHAP. The present study found that the recombinant Trx-tHAP fusion protein aggregated during purification which could be prevented through the addition of 0.2% CHAPS. Trx-tHAP fusion protein was processed into a mature form of tHAP (mtHAP) by both autoactivation, and activation with either enterokinase or plasmepsin II. Using gel filtration chromatography as well as sedimentation velocity and equilibrium ultracentrifugation, …
Locust Dynamics: Behavioral Phase Change And Swarming, Chad M. Topaz, Maria R. D'Orsogna, Leah Edelstein-Keshet, Andrew J. Bernoff
Locust Dynamics: Behavioral Phase Change And Swarming, Chad M. Topaz, Maria R. D'Orsogna, Leah Edelstein-Keshet, Andrew J. Bernoff
Chad M. Topaz
Locusts exhibit two interconvertible behavioral phases, solitarious and gregarious. While solitarious individuals are repelled from other locusts, gregarious insects are attracted to conspecifics and can form large aggregations such as marching hopper bands. Numerous biological experiments at the individual level have shown how crowding biases conversion towards the gregarious form. To understand the formation of marching locust hopper bands, we study phase change at the collective level, and in a quantitative framework. Specifically, we construct a partial integrodifferential equation model incorporating the interplay between phase change and spatial movement at the individual level in order to predict the dynamics of …
Aggregation Behavior Of The Southern Chinch Bug (Hemiptera: Blissidae), Karla M. Addesso, Heather J. Mcauslane, Ron Cherry
Aggregation Behavior Of The Southern Chinch Bug (Hemiptera: Blissidae), Karla M. Addesso, Heather J. Mcauslane, Ron Cherry
Agricultural and Environmental Sciences Faculty Research
The southern chinch bug, Blissus insularis Barber, forms dense, multigenerational aggregations in St. Augustinegrass lawns leading to grass death from sap feeding. We conducted laboratory bioassays to better understand the signals responsible for the formation and maintenance of southern chinch bug aggregations. In small arena assays, chinch bugs demonstrated a stronger aggregation response over time and aggregated more often on or beneath St. Augustinegrass leaf blades than on or under artificial leaf-like shelters constructed from white or green paper. In Y-tube olfactometer assays, bugs of different age and sex were attracted to volatiles from mixed-sex chinch bug aggregations and showed …
Haemorheology Of The Eastern Grey Kangaroo And The Tasmanian Devil, Michael Simmonds, Oguz Baskurt, Herbert Meiselman, Michael Pyne, Michael Kakanis, Ekua Brenu, James Keane, Rhys Christy, Sonya Marshall-Gradisnik
Haemorheology Of The Eastern Grey Kangaroo And The Tasmanian Devil, Michael Simmonds, Oguz Baskurt, Herbert Meiselman, Michael Pyne, Michael Kakanis, Ekua Brenu, James Keane, Rhys Christy, Sonya Marshall-Gradisnik
Sonya Marshall-Gradisnik
The blood of two Australian marsupials, the eastern grey kangaroo (Macropus giganteus) and the Tasmanian devil (Sarcophilus harrisii), has been reported to have greater oxygen-carrying capacity (i.e. haemoglobin content) when compared with that of placental mammals. We investigated whether alterations of blood rheological properties are associated with the increased oxygen-carrying capacity of these marsupials. Eastern grey kangaroos (n = 6) and Tasmanian devils (n = 4) were anaesthetised for blood sampling; human blood (n = 6) was also sampled for comparison. Laboratory measurements included blood and plasma viscosity, red blood cell (RBC) deformability, RBC aggregation and the intrinsic tendency of …
Discrimination, Crypticity, And Incipient Taxa In Entamoeba, Avelina Espinosa, Guillermo Paz-Y-Miño-C
Discrimination, Crypticity, And Incipient Taxa In Entamoeba, Avelina Espinosa, Guillermo Paz-Y-Miño-C
Arts & Sciences Faculty Publications
Persistent difficulties in resolving clear lineages in diverging populations of prokaryotes or unicellular eukaryotes (protistan polyphyletic groups) are challenging the classical species concept. Although multiple integrated approaches would render holistic taxonomies, most phylogenetic studies are still based on single-gene or morphological traits. Such methodologies conceal natural lineages, which are considered “cryptic.” The concept of species is considered artificial and inadequate to define natural populations. Social organisms display differential behaviors toward kin than to nonrelated individuals. In “social” microbes, kin discrimination has been used to help resolve crypticity. Aggregative behavior could be explored in a nonsocial protist to define phylogenetic varieties …