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Full-Text Articles in Life Sciences
Co-Cultures Of Pseudomonas Aeruginosa And Roseobacter Denitrificans Reveal Shifts In Gene Expression Levels Compared To Solo Cultures, Crystal A. Conway, Nwadiuto Esiobu, Jose V. Lopez
Co-Cultures Of Pseudomonas Aeruginosa And Roseobacter Denitrificans Reveal Shifts In Gene Expression Levels Compared To Solo Cultures, Crystal A. Conway, Nwadiuto Esiobu, Jose V. Lopez
Marine & Environmental Sciences Faculty Articles
Consistent biosynthesis of desired secondary metabolites (SMs) from pure microbial cultures is often unreliable. In a proof-ofprinciple study to induce SM gene expression and production, we describe mixed “co-culturing” conditions and monitoring of messages via quantitative real-time PCR (qPCR). Gene expression of model bacterial strains (Pseudomonas aeruginosa PAO1 and Roseobacter denitrificans Och114) was analyzed in pure solo and mixed cocultures to infer the effects of interspecies interactions on gene expression in vitro, Two P. aeruginosa genes (PhzH coding for portions of the phenazine antibiotic pathway leading to pyocyanin (PCN) and the RhdA gene for thiosulfate: cyanide sulfurtransferase …