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Life Sciences Commons

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2011

Honors Theses

Theses/Dissertations

University of Richmond

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Xenopus Laevis Transgenic Lines And Their Use In The Study Of Lymph Heart Musculature Development, Diana M. Filtz Apr 2011

Xenopus Laevis Transgenic Lines And Their Use In The Study Of Lymph Heart Musculature Development, Diana M. Filtz

Honors Theses

Lymph hearts are pulsatile organs present in lower vertebrates that propel lymph throughout the body and into the venous system, assisting in the maintenance of fluid homeostasis. In organisms such as frogs, several pairs of lymph hearts develop amidst the somites during the early tadpole stages. Due to the unique structure and function of lymph heart musculature—exhibiting characteristics of both skeletal and cardiac muscle—the origin of these cells remains highly controversial. Studies have found that in Xenopus, the engrailed gene is expressed explicitly in lymph heart muscle cells throughout development. Through designing a transgenic …


Characterization Of Microbial Community Structure In The Octocoral Leptogorgia Virgulata, Blair E. Armistead Apr 2011

Characterization Of Microbial Community Structure In The Octocoral Leptogorgia Virgulata, Blair E. Armistead

Honors Theses

The process of spicule formation in L. virgulata results in a drop in pH, which, if left unregulated, could lead to demineralization and subsequent animal death. Carbonic anhydrase has been suggested as a possible pH regulatory mechanism in L. virgulata tissues. This study focuses on an additional hypothetical mechanism of pH regulation involving the production of urease by endosymbiotic bacteria living within L. virgulata tissue. PCR and DGGE are used as culture-independent methods to characterize facets of microbial community structure on L. virgulata in order to identify one …