Life Sciences Commons^™

A Polymerase Chain Reaction Method For The Detection Of Selenomonas Noxia, Arthuro Mehretu

UNLV Theses, Dissertations, Professional Papers, and Capstones

In recent studies, periodontal health has been linked to being overweight and/or obese. Among common oral bacteria, Selenomonas noxia has been implicated in converting periodontal health to disease. Selenomonas spp. have also been found in gastric ulcers, and were misdiagnosed as Helicobacter -like organisms, but no further studies were conducted. The increasing clinical and epidemiological importance of S. noxia necessitates the development of a rapid detection method. In this study, a TaqMan 16S rRNA based real-time Polymerase Chain Reaction (PCR) method was developed, optimized and evaluated for the rapid and specific detection of S. noxia . The 16S PCR assay …

Effect Of A Work Site Exercise Program On Selected Fitness And Psychological Parameters, Kristen Ann Christiansen

UNLV Theses, Dissertations, Professional Papers, and Capstones

The increasing rates of obesity continue to threaten the vitality of our nation. Health care costs are soaring and chronic diseases are reaching even the youngest populations. Physical activity is an integral component in reversing the obesity epidemic and improving the health of today's workforce. The present study sought to determine the effect of a work site exercise program on select fitness and psychological parameters. Five female employees participated in a six-week exercise program utilizing HealthBeat(TM) outdoor exercise equipment in a circuit fashion for 30 minutes, 2 days per week. Eighty percent of participants were categorized as sedentary or low-active …

Determining The Validity Of The Nintendo Wii Balance Board As An Assessment Tool For Balance, Sabrina Mae Deans

UNLV Theses, Dissertations, Professional Papers, and Capstones

Context. Application of the Nintendo Wii-fit balance board and its games have been used in Physical Therapy clinics, showing success in individuals with neurological disorders, and has been recommended as a minimum baseline assessment of a symptoms checklist and standardized cognitive and balance assessments for concussion management by the NCAA. However, it still faces challenges of being considered a reliable and consistent tool for producing normative data in the allied healthcare. Because there is little to no evidence for the Wii-fit balance board as a valid balance assessment tool for clinical and/or research usage, the significance of this study is …

Retrospective Evaluation Of Microbial Presence In Existing Saliva Repository: A Pcr Based Molecular Survey Of Oral Microbial Populations From Existing Saliva Samples, Jay Ericksen Davis

UNLV Theses, Dissertations, Professional Papers, and Capstones

The overall purpose of this research project is to explore the link between oral health and disease in the University of Nevada, Las Vegas - School of Dental Medicine (UNLV-SDM) clinic patient population. More specifically, the population of interest is the UNLV-SDM orthodontic clinic patient population, which is mostly composed of adults, females, and minorities. The University of Nevada, Las Vegas, School of Dental Medicine, with more than 70,000 active patients and a post-graduate specialty program in Orthodontics is uniquely positioned to perform this type of oral health epidemiology survey, as well as targeted oral microbial testing among populations within …

Combination Of Virb Binding Site Mutations To Evaluate Collective Impact On Icsp Promoter Activity In Shigella Flexneri, Pashtana Usufuzy, Juan C. Duhart, Maria I. Castellanos, Helen Wing

Undergraduate Research Opportunities Program (UROP)

Shigella flexneri is a gram-negative, invasive bacterial pathogen that afflicts the human colonic epithelium, causing shigellosis, an illness triggering severe dysentery. The World Health Organization cites the disease burden of shigellosis near 90 million episodes and 108,000 deaths per year.

The motility and spread of Shigella is modulated by icsP, a virulence gene. The transcription factor VirB positively regulates many virulence genes encoded by the Shigella virulence plasmid. Two distal binding sites of VirB have been shown to regulate the promoter activity of icsP, despite their location of more than 1 kb upstream of the transcription start site. Five VirB …

The Use Of Chloramines To Eradicate Quagga Mussel Larvae, Trea Lacroix, Kumud Acharya

Undergraduate Research Opportunities Program (UROP)

Quagga Mussels, Dressenia bugensis, are a growing problem in the western United States, particularly in their ability to infest underwater infrastructures and clog water intake pipes and screens of power and treatment plants. Chlorine has been found to be the most effective chemical to get rid of veligers (planktonic larval form of quagga mussels) in the pipes. However, chlorine leaves a residue called trihalomethane, which is a carcinogen at higher concentrations. The purpose of this project is to test the effectiveness of an alternate chemical, chloramines (chlorine and ammonia), which leaves behind little to no residual trihalomethane. Upon experimentation with …

Defining The Role Of Nras In Melanoma Maintenance, Sravya T. Challa, Sheri L. Holmen

Undergraduate Research Opportunities Program (UROP)

The incidence of melanoma has increased 600 percent over the last four decades; it is the most rapidly increasing malignancy among young people in the United States and is currently the leading cause of cancer death in women aged 25- 29. If detected early, the disease is easily treated; however, once the disease has metastasized it is largely refractory to conventional therapies and is associated with a high mortality rate. The development of cancer from a pre-malignant primary tumor to a metastatic cancer that develops at secondary sites is a multi-step process, thought to require many genetic and epigenetic events …

Cell Migration Dynamics After Alteration Of Cell-Cell Contacts In Fibrosarcoma And Glioblastoma Cell Lines, Hassan S. Rizvi, Ronald K. Gary

Undergraduate Research Opportunities Program (UROP)

Cell migration is a vital component of metastasis. In this study, our intent was to study cell migration by alteration of the Wnt/GSK-3 Pathway. Since BeSO4 is a known GSK-3 kinase inhibitor, we hypothesized that this agent would cause cell migration to decrease as a result of β-catenin stabilization. Two human cell lines, HT-1080 (fibrosarcoma) and A172 (glioblastoma), were used to observe migration levels in the presence and absence of BeSO4. Our results show that cell migration is diminished for cells that were pre-treated with BeSO4, in comparison to the untreated (control) cells.

Regulation Of The Shigella Flexneri Icsp Gene And H-Ns Dependent Repression, Rosa Ojeda, Amanda Wigley, Dustin Harrison, Helen Wing

Undergraduate Research Opportunities Program (UROP)

The gram negative bacterium Shigella flexneri is known to cause dysentery in humans and primates. In order to help prevent the spread of shigellosis, gene regulation must be understood. Studies show that the virulence genes in S .flexneri are thermo regulated. At 30°C histone-like nucleoid structuring protein (H-NS) represses transcription of virulence genes and at 37°C VirB derepresses virulence genes. One of the genes that contribute to the virulence of S. flexneri is icsP. My project focuses on the regulation of the S. flexneri icsP gene and has two main goals. The first is to identify the sequence of H-NS …

Hivtoolbox, An Integrated Web Application For Investigating Hiv, David P. Sargeant, Sandeep Deverasetty, Yang Luo, Angel Villahoz Baleta, Stephanie Zobrist, Viraj Rathnayake, Jacqueline C. Russo, Jay Vyas, Mark A. Muesing, Martin Schiller

Life Sciences Faculty Research

Many bioinformatic databases and applications focus on a limited domain of knowledge federating links to information in other databases. This segregated data structure likely limits our ability to investigate and understand complex biological systems. To facilitate research, therefore, we have built HIVToolbox, which integrates much of the knowledge about HIV proteins and allows virologists and structural biologists to access sequence, structure, and functional relationships in an intuitive web application. HIV-1 integrase protein was used as a case study to show the utility of this application. We show how data integration facilitates identification of new questions and hypotheses much more rapid …

Examining Activity Levels And Motor Proficiency: A Comparison Of Children Who Are Overweight And At A Healthy Weight To Their Parents And Peers, Aaron Copeland, Maresa Madsen, Riley Phelps, Brandon Richards

UNLV Theses, Dissertations, Professional Papers, and Capstones

Objective: The purpose of this study was to examine relationships and differences between motor proficiency, activity level, and parental activity level in children who are at a healthy weight and children who are overweight or obese.

Methods: Forty-four children (26 children at a healthy weight and 18 children who were overweight or obese) between the ages of 8-16 (BMI: 14.3-43.6 kg/m2) and 36 parents (BMI: 18.1-44.7) participated in this study. Children and parents wore StepWatch activity monitors (SAM) to measure activity levels over a 72 hour period. Tests reflecting several determinants of motor performance were also administered to …

Effects Of Environment On Children's Motor Scores, Eligibility Status, And Administration Times, Derrick Mittelstadt, Abigail Parker, Kirsten Pickett, Heather Temkin

UNLV Theses, Dissertations, Professional Papers, and Capstones

Best practices for assessing developmental skills in young children focus on naturalistic observation in everyday settings, but the effects of environment on test scores, eligibility status and administration time have not been explored. The Peabody Developmental Motor Scales-Second Edition (PDMS-2) was administered to 34 children aged 18 to 59 months in natural and pull-out settings. PDMS-2 total, gross, and fine motor quotient (TMQ, GMQ, and FMQ) scores were significantly lower in the natural environment (p’s≤.014). Based on our results, more children would qualify for services when tested in natural environments using TMQ and GMQ scores. It also took significantly longer …

The Acute Effects Of Various Types Of Stretching (Static, Dynamic, Ballistic, And No Stretch) Of The Iliopsoas On 40-Yard Sprint Times In Non-Athletes, Scott David Christensen, Craig Perry, Ryan Richard Resnik

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study was to determine the effect of static, ballistic, dynamic, and no stretching immediately prior to a 40-yard sprint in college students. There were 35 healthy subjects (22 male and 13 female) between the ages of 24 and 37 (Mean = 26.46 yrs, SD = 2.99 yrs) who participated. The experiment consisted of running 4, 40-yard sprint trials immediately following 1 of 4 different stretching protocols. Prior to each 40- yard sprint trial, a 5-minute warm up was performed at 3.5 mph on a treadmill. Each subject received each of the four techniques in a randomized …

Effect Of Aquastretch On Range Of Motion At Knee Joint In Total Knee Arthroplasty Patients, Raja Devinder Kochar

UNLV Theses, Dissertations, Professional Papers, and Capstones

The success of the Total Knee Arthroplasty (TKA) depends upon its functional outcomes of increase in reduction in pain, and gait ambulation and performing functional activities of daily living. Compromised range of motion, pain and weakness can reduce their ability to perform activities of daily living. Aquatic Physical Therapy may offer an alternative intervention to traditional Physical Therapy Rehabilitation and many studies have shown improvements in range of motion, swelling, pain reduction, stiffness and quality of life after TKA. AquaStretch, a relatively new form of aquatic therapy, claims to restore the functional mobility which is lost restrictions caused due to …

The Acute Effects Of Static Stretching Of The Gastrocnemius On Limits Of Stability In Young Adults Versus Elderly Adults, Matt Bugnet, Kirk Player

UNLV Theses, Dissertations, Professional Papers, and Capstones

Background and Purpose: Balance is an important part of everyday life for all individuals with many body systems interacting to achieve optimal balance. Proprioceptive organs aid in this interaction, while also acting as stretch receptors. Therefore, it is possible that stretching may influence the overall balance of the individual. The purpose of this study was to investigate the acute effects of stretching on dynamic balance of healthy young and elderly adults. The two groups were used to determine if stretching may discriminately affect balance at different ages.

Subjects: Thirty healthy adults between the ages of 18 and 35 …

The Self: Your Own Worst Enemy? A Test Of The Self-Invoking Trigger Hypothesis, Bradley J. Mckay

UNLV Theses, Dissertations, Professional Papers, and Capstones

The self invoking trigger hypothesis was recently proposed by Wulf and Lewthwaite (2010) as the mechanism underlying the robust effects of attentional focus on motor learning and performance. The hypothesis suggests that causing individuals to access their self schema will negatively impact their ability to learn and perform a motor skill. The purpose of the present study was to provide an initial test of this hypothesis by causing one group of participants to activate their self schema in a straightforward manner. Participants (N = 32) were assigned to either a self-activated or control condition and asked to practice a wiffleball …

The Effect Of Acute Exercise-Induced Fatigue On Pistol Shooting Performance In Police Officers, Melissa Jean Brown

UNLV Theses, Dissertations, Professional Papers, and Capstones

Duties of police officers include apprehending suspects, which may involve foot pursuit and firing a weapon at a suspect. Previous studies in biathletes and soldiers suggest that shooting performance is compromised when fatigue is present. Purpose: To investigate the effects of exercise-induced fatigue on shooting performance in police officers. Methods: Nine police officers fired five rapid-fire shots at three targets each with a fifteen second rest period in between each trail. Officers then exercised on a cycle ergometer to 85% maximum heart rate or voluntary fatigue. The pretest shooting was repeated post-exercise. Distances of 4 shots from the center of …

Event Program, Carl Reiber, Nicholle Booker

Festival of Communities: UG Symposium (Posters)

UNLV Undergraduates from all departments, programs and colleges participated in a campus-wide symposium on April 16, 2011. Undergraduate posters from all disciplines and also oral presentations of research activities, readings and other creative endeavors were exhibited throughout the festival.

2011-2012 Unlv Mcnair Journal, Cyndy Anang, Sajar Camara, Pamela Cornejo, Carla Antonieta Farcello, Ilse Anahi Garcia, Natiera Magnuson, William L. Mccurdy, Lorena Munoz, Maxym V. Myroshnychenko, Ricardo Rios, Theodore Waldeck, Barbara Wallen, Ana Zuniga, Brenda M. Aguilar, Tiffany Alexandra Alvarez, Daniel N. Erosa, Paige C. Espinosa, Carla Antonieta Farcello, Julienne Jochel Paraiso, Nathaniel Derek Phillipps, Carmen Vallin, Jacent N. Wamala, Ernesto Zamora-Ramos

McNair Journal

Journal articles based on research conducted by undergraduate students in the McNair Scholars Program

Table of Contents

Biography of Dr. Ronald E. McNair

Statements:

Dr. Neal J. Smatresk, UNLV President

Dr. Juanita P. Fain, Vice President of Student Affairs

Dr. William W. Sullivan, Associate Vice President for Retention and Outreach

Mr. Keith Rogers, Deputy Executive Director of the Center for Academic Enrichment and Outreach

McNair Scholars Institute Staff

Male Anorexia: In The Modern 2011, Barbara Wallen-Adams

McNair Poster Presentations

It has been reported that homosexuality is a risk factor for males with anorexia. However, it is unclear whether it is a specific risk factor for eating pathology or just a common risk factor associated with psychopathology. If social stigmatization of homosexuality can cause general psychological suffering that express itself as a discomfort with sexual orientation, poor self-esteem, depression, and disordered eating, than homosexuality may act as a general risk factor in itself. It can also be interrelated to certain aspects that distinctively increase the risk for males developing anorexia, such as increased identification with the male gender roles (Munen …