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Detection Of Panax Quinquefolius In Panax Ginseng Using ‘Subtracted Diversity Array’, Linhai Niu, Nitin Mantri, Chun Li, Charlie Xue, Hans Wohlmuth, Edwin Pang
Detection Of Panax Quinquefolius In Panax Ginseng Using ‘Subtracted Diversity Array’, Linhai Niu, Nitin Mantri, Chun Li, Charlie Xue, Hans Wohlmuth, Edwin Pang
Dr Hans Wohlmuth
BACKGROUND: Food adulteration remains a major global concern. DNA fingerprinting has several advantages over chemical and morphological identification techniques. DNA microarray-based fingerprinting techniques have not been used previously to detect adulteration involving dried commercial samples of closely related species. Here we report amplification of low-level DNA obtained from dried commercial ginseng samples using the Qiagen™ REPLI-g® Kit. Further, we used a subtracted diversity array (SDA) to fingerprint the two ginseng species, Panax ginseng and Panax quinquefolius, that are frequently mixed for adulteration. RESULTS: The two ginseng species were successfully discriminated using SDA. Further, SDA was sensitive enough to detect a …