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- Keyword
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- 4-way junction; Dna tri component probe; Molecular beacon probe; Secondary structured dna (1)
- Adrenergic hormones; Cardiac conduction; Cardiac rhythm; Cardiac rhythmicity; Heart conduction; Heart rhythm; Heart rhythmicity; Retinoic acid; Stress hormones (1)
- Affinity chromatography; Ms 818; Sds page (1)
- Androgen receptor; Bicalutamide; Casodex; Microrna; Mirna; Prostate cancer (1)
- Anthrax; Chloroplast; Diabetes; Pharmaceutical; Transformation; Transgenic; Vaccine (1)
Articles 1 - 8 of 8
Full-Text Articles in Life Sciences
Stimulator Of Neurotropic Effects Determining The Mechanism Of Action Of The Ms-818 Compound Through Protein Identification By Affinity Chromatography And Sds-Page, Charlene Seraphina Dass
Stimulator Of Neurotropic Effects Determining The Mechanism Of Action Of The Ms-818 Compound Through Protein Identification By Affinity Chromatography And Sds-Page, Charlene Seraphina Dass
HIM 1990-2015
The MS-818 compound is used in the proliferation process of neuronal cells and many biological activities that accompany this process such as astrocyte differentiation, inhibition of neuronal apoptosis, and fraction repairs. We do know the effects of this compound, but the mechanism of action remained uncertain until now. To determine the pathway of this compound, NT2 cells were cultured and lysed to isolate the proteins. Affinity Chromatography was performed in order to immobilize the MS-818 compound to a Hi-Trap NHS column. The NT2 protein sample was injected through the column and eluted with a MS-818 concentrated, high salt content elution …
Identification Of Physiological Substrates Of Plasmodium Falciparum Pfpk5, A Cdk-Like Kinase, Catherine Sullenberger
Identification Of Physiological Substrates Of Plasmodium Falciparum Pfpk5, A Cdk-Like Kinase, Catherine Sullenberger
HIM 1990-2015
Malaria is one of the most devastating infectious diseases causing 1-3 million fatalities a year. The majority of these cases occur amongst children in developing countries. Malarial strains in these areas are exhibiting increasing resistance to canonical treatments proving the importance of new drug targets for anti-malarials. Identification of new drug targets is dependent upon a better understanding of the molecular biology of the parasitic agent of malaria, Plasmodium. The regulation of Plasmodium's complex life cycle is still not well understood. Elucidation of signaling pathways involved in Plasmodium cell cycle regulation will provide insights into how the parasite thrives in …
Microrna Regulation Of Prostate Cancer Desensitization To Androgen Receptor Antagonist Drugs During Androgen Deprivation Therapy, Robert A. Lorch
Microrna Regulation Of Prostate Cancer Desensitization To Androgen Receptor Antagonist Drugs During Androgen Deprivation Therapy, Robert A. Lorch
HIM 1990-2015
The current standard treatment of prostate cancer by androgen deprivation therapy involves using drugs such as bicalutamide (Casodex) to antagonistically block androgen receptors that are normally present within prostate cells. Usually, the therapy is successful in the short run at limiting the growth of prostate cancer. However, in virtually all cases tumors begin to grow aggressively again after several months of treatment and new therapies must be started. The mechanism by which these prostate cells transform from androgen sensitive to androgen independent and anti-androgen resistant is unclear. In this study, we investigated the role of microRNAs, small 15 to 18 …
Cross-Talk Of Retinoic Acid And Adrenergic Hormone Signaling May Influence Development Of Cardiac Conduction And Rhythmicity In Utero, Sabikha Alam
HIM 1990-2015
Stress hormones, adrenaline and noradrenaline, have been shown to be critical for heart development. Mice lacking dopamine greek lower case letter beta]-hydroxylase (Dbh), an enzyme responsible for synthesis of these adrenergic hormones, die during mid-gestation due to cardiac failure. Prior research showed that adrenergic cells are found within the electrical conduction system of the heart, and adrenergic deficiency leads to slowed cardiac conduction during embryogenesis. Microarray analysis of wild-type (Dbh+/+) and knockout (Dbh-/-) mouse hearts revealed significant differences in expression of retinoic acid (RA) signaling genes. RA signaling has also been shown to be critical for heart development. These data …
Analysis Of The Repair Of Topoisomerase Ii Dna Damage, Eric D. Goldstein
Analysis Of The Repair Of Topoisomerase Ii Dna Damage, Eric D. Goldstein
HIM 1990-2015
A large number of anti-cancer chemotherapeutics target DNA topoisomerases. Etoposide is a specific topoisomerase II poison which causes reversible double strand DNA breaks. The focus of this project is to analyze the repair of DNA damage induced by etoposide.. Double strand DNA break repair is mediated by through either non-homologous end joining (NHEJ) or homologous recombination. NHEJ repairs through direct ligation of a double stranded break while homologous recombination utilizes a homologous template to recover the wild type sequence. A reporter cassette, RYDR-GFP, has been stably integrated into HeLa cells. This reporter contains an ultra-high affinity topoisomerase II cleavage site …
A Tripartile Biosensor For Real-Time Snss Detection In Dna Hairpin Motif, Camha Nguyen
A Tripartile Biosensor For Real-Time Snss Detection In Dna Hairpin Motif, Camha Nguyen
HIM 1990-2015
The hybridization between two complementary strands of nucleic acid is the basis for a number of applications in DNA and RNA analysis, including in vivo RNA monitoring, microarrays, SNPs detection, and so on. The short oligonucleotide probes form Watson/Crick base pairs (A-T and G-C) with the analyzed nucleic acid. Molecular beacon (MB) probe is one of the most advantageous tools for nucleic acid analysis in real-time. A traditional MB probe consists of a DNA strand folded in hairpin motif with a fluorophore attached to the 5'end and a quencher attached to the 3' end. The loop segment is complementary to …
Plant-Made Oral Vaccines Evaluation Of Capsules, James Stewart New
Plant-Made Oral Vaccines Evaluation Of Capsules, James Stewart New
HIM 1990-2015
Antigen expression through the Chloroplast Transformation Technology (CTT) produces bioencapsulated subunit-vaccines, capable of eliciting immune responses when delivered orally. Considerable challenges to effective plant-based vaccines are the normalization of dosage and preservation of accumulated antigen, which is complicated by variable high water content and protease activity. This study critically examines the efficacy of lyophilization in dehydrating plant-tissues and preserving plant-derived antigens with vaccine potential. Lyophilization was optimized through gravimetric analysis using lettuce expressing Protective Antigen (PA) of Bacillus anthracis (LS-HPAG) and the human autoantigen Proinsulin (Pins) fused to Cholera toxin subunit B (LS-CTB-Pins). Lyophilization for 48-hours was sufficient treatment to …
Cerium Oxide Nanoparticles For The Detection Of Antimicrobial Resistance, Alexander J. Noll
Cerium Oxide Nanoparticles For The Detection Of Antimicrobial Resistance, Alexander J. Noll
HIM 1990-2015
The rise of antimicrobial resistance demands the development of more rapid screening methods for the detection of antimicrobial resistance in clinical samples to both give the patient the proper treatment and expedite the treatment of patients. Cerium oxide nanoparticles may serve a useful role in diagnostics due to their ability to exist in a mixed valence state and act as either oxidizing agents or reducing agents. Considering that cerium oxide nanoparticles have been shown to shift in absorbance upon oxidation, a useful method of antimicrobial resistance detection could be based on the oxidation of cerium oxide nanoparticles. Herein, an assay …