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Pathogenic Role Of Phosphodiesterase Type 5 Upregulation In Cardiac Ischemia/Reperfusion Injury, Daniel Hobbs
Pathogenic Role Of Phosphodiesterase Type 5 Upregulation In Cardiac Ischemia/Reperfusion Injury, Daniel Hobbs
Theses and Dissertations
Phosphodiesterase Type 5 (PDE5) inhibitors are cardioprotective against ischemia/reperfusion (I/R) injury. However, it remains uncertain if I/R affects PDE5. We hypothesized that generation of reactive oxygen species (ROS) during I/R leads to upregulation of PDE5, which contributes to pathological changes following acute myocardial infarction (AMI). Adult male ICR mice were subjected to 30 minutes of in vivo or ex vivo I/R. To examine the role of ROS, a subset of hearts were perfused with 100 µM hydrogen peroxide (H2O2). Expression and activity of PDE5, pPDE5, and cGMP-dependent protein kinase (PKG) were measured by Western blots and spectrophotometric assay. The results …