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Enzyme Entrapment In Reprecipitated Polyaniline Nano- And Microparticles, Louis R. Nemzer, Austin Schwartz, Arthur Epstein
Enzyme Entrapment In Reprecipitated Polyaniline Nano- And Microparticles, Louis R. Nemzer, Austin Schwartz, Arthur Epstein
Louis R Nemzer
We introduce a novel method for fabricating nano- and microscale polyaniline particles containing an entrapped oxidoreductase enzyme for use in biosensing applications. This facile process utilizes the reprecipitation of the emeraldine base form of polyaniline from an aqueous-organic suspension, with hydrophobic collapse and subsequent cross-linking of the polymer induced by adjusting the ionic strength beyond a critical threshold. We present UV-vis spectroscopy data, including a quantitative treatment of the spectral line width, along with dynamic light scatting results, to explain the conformation changes in the polyaniline chains that accompany this transition. The resultant aggregated supermolecular polyaniline formations immobilize enzymes via …
A Polyaniline-Based Optical Biosensing Platform Using An Entrapped Oxidoreductase Enzyme, Louis R. Nemzer, Arthur Epstein
A Polyaniline-Based Optical Biosensing Platform Using An Entrapped Oxidoreductase Enzyme, Louis R. Nemzer, Arthur Epstein
Louis R Nemzer
A novel optical biosensing platform utilizing the unique solubility and chemochromic properties of polyaniline is presented. A facile, ion-induced reprecipitation method leads to the entrapment of a chosen oxidoreductase enzyme, which, in the presence of its associated substrate, catalyzes a reversible redox change in the host polymer. This change is monitored via the UV–vis absorption and subsequently analyzed to fit a Michaelis–Menten model. Here, in vitro prototype devices demonstrate selective sensing of glucose, choline, and uric acid, and the potential to be adapted for use as part of real-time in vivo monitoring systems is discussed