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Genomic Tools Development For Aquilegia: Construction Of A Bac-Based Physical Map, Guang-Chen Fang, Barbara P. Blackmon, David C. Henry, Margaret E. Staton, Christopher Saski, Scott A. Hodges, Jeff P. Tomkins, Hong Luo Nov 2010

Genomic Tools Development For Aquilegia: Construction Of A Bac-Based Physical Map, Guang-Chen Fang, Barbara P. Blackmon, David C. Henry, Margaret E. Staton, Christopher Saski, Scott A. Hodges, Jeff P. Tomkins, Hong Luo

Publications

The genus Aquilegia, consisting of approximately 70 taxa, is a member of the basal eudicot lineage, Ranuculales, which is evolutionarily intermediate between monocots and core eudicots, and represents a relatively unstudied clade in the angiosperm phylogenetic tree that bridges the gap between these two major plant groups. Aquilegia species are closely related and their distribution covers highly diverse habitats. These provide rich resources to better understand the genetic basis of adaptation to different pollinators and habitats that in turn leads to rapid speciation. To gain insights into the genome structure and facilitate gene identification, comparative genomics and whole-genome shotgun …


Complete Plastid Genome Sequences Of Three Rosids (Castanea, Prunus, Theobroma): Evidence For At Least Two Independent Transfers Of Rpl22 To The Nucleus, Robert K. Jansen, Christopher Saski, Seung-Bum Lee, Anne K. Hansen, Henry Daniell Oct 2010

Complete Plastid Genome Sequences Of Three Rosids (Castanea, Prunus, Theobroma): Evidence For At Least Two Independent Transfers Of Rpl22 To The Nucleus, Robert K. Jansen, Christopher Saski, Seung-Bum Lee, Anne K. Hansen, Henry Daniell

Publications

Functional gene transfer from the plastid to the nucleus is rare among land plants despite evidence that DNA transfer to the nucleus is relatively frequent. During the course of sequencing plastid genomes from representative species from three rosid genera (Castanea, Prunus, Theobroma) and ongoing projects focusing on the Fagaceae and Passifloraceae, we identified putative losses of rpl22 in these two angiosperm families. We further characterized rpl22 from three species of Passiflora and one species of Quercus and identified sequences that likely represent pseudogenes. In Castanea and Quercus, both members of the Fagaceae, we identified a …


Oil And Gas Impacts In The Big Cypress Ecosystem: An Analysis Of Impacts Associated With Proposed Activities In The Nobles Grade Area, William Conner, Stephen E. Davis Iii, Kirsten N. Hines, John J. Cox, Dale E. Gawlik, Jerome A. Jackson, James O. Jones, Fernando Miralles-Wilhelm, Jennifer H. Richards Aug 2010

Oil And Gas Impacts In The Big Cypress Ecosystem: An Analysis Of Impacts Associated With Proposed Activities In The Nobles Grade Area, William Conner, Stephen E. Davis Iii, Kirsten N. Hines, John J. Cox, Dale E. Gawlik, Jerome A. Jackson, James O. Jones, Fernando Miralles-Wilhelm, Jennifer H. Richards

Publications

No abstract provided.


Six New Species And 1 New Species Record Of Orthotrichia (Trichoptera: Hydroptilidae) From China, Zhou Lei, Yang Lian-Fang, John C. Morse Aug 2010

Six New Species And 1 New Species Record Of Orthotrichia (Trichoptera: Hydroptilidae) From China, Zhou Lei, Yang Lian-Fang, John C. Morse

Publications

Six new species of the caddisfly genus Orthotrichia are described and illustrated from China, namely Or. apophysis Zhou & Yang, sp. nov.; Or. latiramifera Zhou & Yang, sp. nov.; Or. cornuta Zhou &Yang, sp. nov.; Or. discedata Zhou & Morse, sp. nov.; Or. cuspidigera Zhou & Yang, sp. nov.; and Or. subrhomba Zhou & Morse, sp. nov.. One species, Or. terpsichore Malicky & Chantaramongkol, 2007, is recorded for the first time from China, bringing the number of species of the genus known from China to 14. Of these, 10 species are distributed among 3 species groups of Marshall (1979), with …


Four New Species And Two New Records Of Polyplectropus From China (Trichoptera: Polycentropodidae), Hua Zhong, Lian-Fang Yang, John C. Morse Apr 2010

Four New Species And Two New Records Of Polyplectropus From China (Trichoptera: Polycentropodidae), Hua Zhong, Lian-Fang Yang, John C. Morse

Publications

Four new species and 2 new records of the genus Polyplectropus Ulmer from China are described and illustrated. The new species include Polyplectropus cubitalis Zhong and Yang sp. nov., from Guizhou, Polyplectropus subteres Zhong and Yang sp. nov., from Jiangxi and Zhejiang, Polyplectropus tridentatus Zhong and Morse sp. nov., from Guangxi, and Polyplectropus tianmushanensis Zhong and Yang, sp. nov., from Zhejiang. Two species, Polyplectropus ahas Malicky and Chantaramongkol, 1993, and Polyplectropus anakgugur Malicky, 1995, are newly recorded from China, bringing the number of Chinese Polyplectropus species to 28. The newly recognized Polyplectropus anakgugur Group is probably monophyletic, as evidenced by …


Defining The Genus Hydropsyche (Trichoptera:Hydropsychidae) Based On Dna And Morphological Evidence, Christy Jo Geraci, Xin Zhou, John C. Morse, Karl M. Kjer Mar 2010

Defining The Genus Hydropsyche (Trichoptera:Hydropsychidae) Based On Dna And Morphological Evidence, Christy Jo Geraci, Xin Zhou, John C. Morse, Karl M. Kjer

Publications

In this paper, we review the history of Hydropsychinae genus-level classification and nomenclature and present new molecular evidence from mitochondrial cytochrome c oxidase subunit I (COI) and nuclear large subunit ribosomal ribonucleic acid (28S) markers supporting the monophyly of the genus Hydropsyche. Both molecular and morphological characters support a broad conservative definition of Hydropsyche. Caledopsyche, Hydatomanicus, and Occutanspsyche are synonymized with Hydropsyche. The following species groups are established: Hydropsyche bronta Group (generally corresponding with Ceratopsyche and Hydropsyche morosa and newae Groups), Hydropsyche colonica Group (generally corresponding with Orthopsyche), Hydropsyche instabilis Group (generally corresponding with Hydropsyche s.s.), and Hydropsyche naumanni Group …


Sucrose, Water And Nutrient Use During State Ii Multiplication Of Two Turmeric Clones (Curcuma Longa L.) In Liquid Medium, Jeffrey Adelberg Mar 2010

Sucrose, Water And Nutrient Use During State Ii Multiplication Of Two Turmeric Clones (Curcuma Longa L.) In Liquid Medium, Jeffrey Adelberg

Publications

Two clones of turmeric (Cucruma longa L.) were assessed nine times during a 28-day culture period in double phosphate MS liquid medium containing 2%, 4% and 6% sucrose. The phase of rapid shoot bud multiplication lasted for 21 days or less, in both clones in all media. The maximum multiplication rate was 3.4× at 20 and 19 days, with 3.9% or 4.7% initial sucrose concentration being optimal for the clones 9-3 and 35-1, respectively. Dry mass increased over the entire culture period and was greatest with 4.8% or at least 6% initial sucrose concentration for the two clones, 9-3 …


Evolution Of Terrestrial Habitat In Manophylax Species (Trichoptera:Apataniidae), With A New Species From Alaska, Suvtsetseg Chuluumbat, John C. Morse, Joanna L. Lessard, M Eric Benbow, Matthew D. Wesener, John Hudson Feb 2010

Evolution Of Terrestrial Habitat In Manophylax Species (Trichoptera:Apataniidae), With A New Species From Alaska, Suvtsetseg Chuluumbat, John C. Morse, Joanna L. Lessard, M Eric Benbow, Matthew D. Wesener, John Hudson

Publications

The genus Manophylax (Trichoptera:Apataniidae) contains 3 species from the Nearctic Region and 3 species from Japan. A 7th species of Manophylax new for science is described and illustrated from Alaska. Habitats of larvae and pupae of the different Manophylax species are either mostly hygropetric or mostly terrestrial. Phylogenetic analysis infers 3 principal species groups occurring in the eastern and western Nearctic and eastern Palearctic Biogeographic Regions. The habitat of the western Nearctic M. annulatus Species Group is mostly hygropetric and that of the eastern Nearctic M. altus and eastern Palearctic M. futabae Species Groups are mostly terrestrial. The phylogeny suggests …


Localization Of Phosphatidylinositol (3,4,5)-Trisphosphate To Phagosomes In Entamoeba Histolytica Achieved Using Glutathione S-Transferase- And Green Fluorescent Protein-Tagged Lipid Biosensors, Yevgeniya A. Byekova, Rhonda R. Powell, Brenda H. Welter, Lesly A. Temesvari Jan 2010

Localization Of Phosphatidylinositol (3,4,5)-Trisphosphate To Phagosomes In Entamoeba Histolytica Achieved Using Glutathione S-Transferase- And Green Fluorescent Protein-Tagged Lipid Biosensors, Yevgeniya A. Byekova, Rhonda R. Powell, Brenda H. Welter, Lesly A. Temesvari

Publications

Entamoeba histolytica is an intestinal protozoan parasite that causes amoebic dysentery and liver abscess. Phagocytosis by the parasite is a critical virulence process, since it is a prerequisite for tissue invasion and establishment of chronic infection. While the roles of many of the proteins that regulate phagocytosis-related signaling events in E. histolytica have been characterized, the functions of lipids in this cellular process remain largely unknown in this parasite. In other systems, phosphatidylinositol (3,4,5)-trisphosphate (PIP3), a major product of phosphoinositide 3 kinase (PI3-kinase) activity, is essential for phagocytosis. Pleckstrin homology (PH) domains are protein domains that specifically bind …


A Comparative Identification Of Ochratoxin A In Longan Fruit Pulp By High Performance Liquid Chromatography-Fluorescence Detection And Electron Spray Ionization-Mass Spectrometry, Jing Li, Haihui Xie, Bao Yang, Xinhong Dong, Linyan Feng, Feng Chen, Yueming Jiang Jan 2010

A Comparative Identification Of Ochratoxin A In Longan Fruit Pulp By High Performance Liquid Chromatography-Fluorescence Detection And Electron Spray Ionization-Mass Spectrometry, Jing Li, Haihui Xie, Bao Yang, Xinhong Dong, Linyan Feng, Feng Chen, Yueming Jiang

Publications

Harvested longan (Dimocarpus longan Lour.) fruit are susceptible to decay caused by both bacterial and fungal infections. Ochratoxin A (OTA) is a kind of mycotoxin produced by a number of fungi. In this study, OTA was extracted from longan fruit pulp by 80% methanol and then loaded on C-18 solid-phase extraction columns. The extract solution was then analyzed by high-performance liquid chromatography - fluorescence detection (HPLC-FD) and an electron spray ionization-mass spectrometry (ESI-MS), respectively. The HPLC-FD analysis showed that a compound similar to OTA might exist in longan fruit pulp, but further analysis by the ESI-MS method demonstrated that …


Two Novel Approaches Used To Produce Biodiesel From Low-Cost Feedstocks, Xiaohu Fan, Xi Wang, Feng Chen Jan 2010

Two Novel Approaches Used To Produce Biodiesel From Low-Cost Feedstocks, Xiaohu Fan, Xi Wang, Feng Chen

Publications

The price of feedstock is one of the most significant factors affecting the economic viability of biodiesel manufacturer. Many approaches were investigated to reduce the biodiesel production cost. The present work gave a preliminary study of two approaches to economically produce biodiesel from waste cooking oil (WCO) and flaked cottonseed. One was the use of ultrasound-assisted synthesis of biodiesel from WCO. The other was the application of in situ transesterification from flaked cottonseed. Gas chromatography (GC) and High performance liquid chromatography (HPLC) results demonstrated the feasibility of using both approaches to produce biodiesel from low-cost feedstock.


Contrasting Patterns Of Phylogeographic Relationships In Sympatric Sister Species Of Ironclad Beetles (Zopheridae: Phloeodes Spp.) In California's Transverse Ranges, Michael S. Caterino, Maxi Polihronakis Jan 2010

Contrasting Patterns Of Phylogeographic Relationships In Sympatric Sister Species Of Ironclad Beetles (Zopheridae: Phloeodes Spp.) In California's Transverse Ranges, Michael S. Caterino, Maxi Polihronakis

Publications

Comparative phylogeography of sympatric sibling species provides an opportunity to isolate the effects of geography and demographics on the evolutionary history of two lineages over the same, known time scale. In the current study, we investigated the phylogeographic structure of two zopherid beetle species, Phloeodes diabolicus and P. plicatus, where their ranges overlap in California's Transverse Ranges.