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Regulation Of Heterochromatin Assembly On Unpaired Chromosomes During Caenorhabditis Elegans Meiosis By Components Of A Small Rna-Mediated Pathway, Xingyu She, Xia Xu, Alexander Fedotov, William G. Kelly, Eleanor M. Maine
Regulation Of Heterochromatin Assembly On Unpaired Chromosomes During Caenorhabditis Elegans Meiosis By Components Of A Small Rna-Mediated Pathway, Xingyu She, Xia Xu, Alexander Fedotov, William G. Kelly, Eleanor M. Maine
Biology - All Scholarship
Many organisms have a mechanism for down regulating the expression of non-synapsed chromosomes and chromosomal regions during meiosis. This phenomenon is thought to function in genome defense. During early meiosis in Caenorhabditis elegans, unpaired chromosomes (e.g., the male X chromosome) become enriched for a modification associated with heterochromatin and transcriptional repression, dimethylation of histone H3 on lysine 9 (H3K9me2). This enrichment requires activity of the cellular RNA-directed RNA polymerase, EGO-1. Here we use genetic mutation, RNA interference, immunofluorescence microscopy, fluorescence in situ hybridization, and molecular cloning methods to identify and analyze three additional regulators of meiotic H3K9me2 distribution: CSR-1 (a …