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Oxidative Stress, Calcium Homeostasis, And Altered Gene Expression In Human Lung Epithelial Cells Exposed To Zno Nanoparticles, Chuan-Chin Huang
Oxidative Stress, Calcium Homeostasis, And Altered Gene Expression In Human Lung Epithelial Cells Exposed To Zno Nanoparticles, Chuan-Chin Huang
Masters Theses
"The influence of 20 nm ZnO nanoparticles on oxidative stress, intracellular calcium homeostasis, and gene expression was studied in human bronchial epithelial cells (BEAS-2B). ZnO caused a concentration- and time-dependent cytotoxicity while elevating oxidative stress and causing membrane damage (cellular LDH release). There was a remarkably steep relationship between concentration and toxicity at concentrations from 5 to 10 μg/ml. Exposure to ZnO increased intracellular calcium levels in a concentration- and time-dependent manner. Treatment with the antioxidant N-acetylcysteine prevented cell loss and diminished the increase in intracellular calcium concentration, suggesting oxidative stress mediated cytotoxicity. Exposure to a sublethal concentration of ZnO …