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Full-Text Articles in Life Sciences
Investigating The Oligomerization Of Vitronectin, Yacynth Ruwansara
Investigating The Oligomerization Of Vitronectin, Yacynth Ruwansara
Masters Theses
Vitronectin is a multi-functional glycoprotein that is present in the plasma and extra-cellular matrix of eukaryotes. It is capable of binding a wide variety of structurally different ligands, including plasminogen activators, plasminogen activator-inhibitors, proteases, cell surface receptors and components of the extra-cellular matrix. Vitronectin exists in two conformations – as a monomer in circulation, and as a multimer in the extracellular matrix. The pathway by which vitronectin undergoes the transition from monomer to multimer is not well characterized, but this laboratory has put forward evidence to suggest that the binding of vitronectin with plasminogen activator inhibitor type-1 (PAI-1), facilitates higher …
Novel Role For The Cdk-Activating Kinase Cak1 In Actively Growing Cells, Su Hwa Kim
Novel Role For The Cdk-Activating Kinase Cak1 In Actively Growing Cells, Su Hwa Kim
Masters Theses
Cdc28 is the main cyclin-dependent kinase (CDK) directing the yeast cell cycle. Besides cyclin binding, Cdc28 requires phosphorylation by Cak1 (CDK-activating kinase) to achieve full activity. In previous work, our laboratory isolated carboxy-terminal cdc28CST mutants that display high temperature sensitivity and chromosome instability. These phenotypes are suppressed by increasing the copy numbers of Cak1 in its catalytically active or inactive form. The cdc28CST mutants are also extremely sensitive to changes in Cak1 integrity and dosage. These results indicated that besides the activating phosphorylation by Cak1, the interaction between Cak1 and the carboxyl terminus of Cdc28 is important to …
Cyp6w1 Gene Of Drosophila Melanogaster: Studies On Its Role In Ddt Resistance And Inducibility By Different Xenobiotic Compounds, Vita Lam
Masters Theses
Cytochrome P450 monooxygenases (CYP), a large family of enzymes, play an important role in insecticide resistance. Compared to the susceptible strains, the resistant strains of Drosophila show higher expression of one or more Cyp genes including Cyp6w1, which maps close to the resistance locus. In the present investigation I examined the Cyp6w1 gene’s role in DDT resistance and inducibility with caffeine, DDT and phenobarbital (PB). Utilizing the Gal4:UAS binary system, I overexpressed CYP6W1 RNA in either a susceptible strain alone or in combination with CYP6A2 or CYP6G1 RNA, which are known to give a low level of DDT resistance. …