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Open Access. Powered by Scholars. Published by Universities.®

2008

University of Tennessee, Knoxville

Animal Sciences

Doctoral Dissertations

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Spermatogonia Stem Cell Dynamics Following Hormonal Induction, Ischemic Disturbance In Vivo Or Proliferation Under In Vitro Culture In Pre- And Postpubertal Bulls, Gustavo M. Schuenemann Aug 2008

Spermatogonia Stem Cell Dynamics Following Hormonal Induction, Ischemic Disturbance In Vivo Or Proliferation Under In Vitro Culture In Pre- And Postpubertal Bulls, Gustavo M. Schuenemann

Doctoral Dissertations

The overall aim of the studies described herein was to evaluate bovine spermatogonial cell dynamics under various conditions. Results from these experiments will provide the basis for potential production of offspring following spermatogonial stem cell transfer. Experiment 1 evaluated gonadotropin administration effects at initiation of inhibin passive immunization in Jersey bull calves on testicular morphology and development. Primary treatments consisted of control (KLH) or immunization (INH) plus a combination of saline, FSH, or GnRH. Administration of FSH at the time of initial immunization against inhibin significantly increased number of germ cells (92.2 ± 9 x 106 cells) compared to INH-Saline …


Prevalence Of Class 1 Integrons And Antibiotic Resistance Patterns In Bacteria Of Swine And Chicken In The Us And Thailand, Sumalee Liamthong May 2008

Prevalence Of Class 1 Integrons And Antibiotic Resistance Patterns In Bacteria Of Swine And Chicken In The Us And Thailand, Sumalee Liamthong

Doctoral Dissertations

Studies were conducted to investigate the prevalence and potential for transfer of class 1 integrons and antimicrobial resistance in bacteria of broiler chickens and swine from the US and Thailand. Antibiograms were characterized and integron sequences were detected using standard methods. To determine if transfer of integrons occurred between bacterial species the location of the integrons (plasmid versus chromosome) was determined, and when integron-positive E. coli and Salmonella isolates possessed identical amplicon patterns, PCR products were sequenced to determine homology. Class 1 integrons were detected in 1,732 of 3,824 isolates from broiler chickens and 1,782 of 4,253 isolates from swine. …