Life Sciences Commons^™

Gene Signature Of Stromal Cells Which Support Dendritic Cell Development., Geneviève Despars, Pravin Periasamy, Jonathan Tan, Janice Abbey, Terence O'Neill, Helen O'Neill

Jonathan Tan

Spleen stromal cells are critical determinants of dendritic cell (DC) development in spleen. The spleen stromal line, namely STX3, supports DC differentiation in vitro from overlaid bone marrow cells while the lymph node stromal line, namely 2RL22, does not. Here we have characterised the hematopoietic support capacity of each stroma, and analyzed lineage origin of the stromal cell lines by gene profiling using microarrays. Stromal co-culture experiments were performed using bone marrow cells as a source of hematopoietic progenitors. A characteristic immature myeloid-like CD11c+CD11b+CD86+MHC-II−/loB220−CD8α− DC is produced after 14 days in STX3 cocultures, while 2RL22 cocultures produce only monocyte/macrophage-like cells. …

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri

Marta Gomez-Chiarri

Postharvest surveillance for Vibrio vulnificus by a commercial processing facility was conducted from May 2001 to September 2003. Harvest areas included the Delaware Bay, Long Island Sound and Prince Edward Island. Occurrence followed a seasonal distribution. Low densities were observed in June, increased through August, and became rare by September. Given the ubiquitous nature of marine Vibrios, it was not surprising to find V. vulnificus everywhere, which was investigated. Observations confirmed the importance of strict time/temperature and product handling practices by shellfish harvesters, dealers and processors. Mishandling led to a potentially dangerous and uncharacteristically high V. vulnificus observation.

Proteome Of Geobacter Sulfurreducens Through Genome-Wide Expression Data And Sequence Analysis, Derek Lovley, Yan-Huai Ding, Kim Hixson, Muktak Aklujkar, Mary Lipton, Richard Smith, Tϋnde Mester

Muktak Aklujkar

No abstract provided.

Sourwood: An Apiforestation Story, Tammy Horn

Tammy Horn

No abstract provided.

Web-Based Arabidopsis Functional And Structural Genomics Resources, Yan Lu, Robert Last

Yan Lu

No abstract provided.

Reflections On Recycling, Pollution And History Or, How To Beat The High Cost Of Living, Richard B. Philp

Richard B. Philp

Lessons learned during the Great Depression and World War II may have to be relearned. The three Rs (reduce, reuse and recycle) were essential then to individual and national survival. A fourth R, repair, was practised as well. These lessons, if relearned, will stand us in good stead when dealing with the current economic climate as well as with problems of pollution and global warming.

A Transcendental To Changing Metal Powder Characteristics, Mahendra Kumar Trivedi, Rama Mohan Tallapragada

Mahendra Kumar Trivedi

• https://www.trivedieffect.com/science/a-transcendental-approach-to-changing-metal-powder-characteristics
• https://www.sciencedirect.com/science/article/pii/S0026065708701450

North Atlantic Vibrio Vulnificus Surveillance From Postharvest Oysters At A Us Shellfish Processing Facility, Kenneth Lavalley, Joseph Dealteris, Michael Rice, Marta Gomez-Chiarri

Michael A Rice

Postharvest surveillance for Vibrio vulnificus by a commercial processing facility was conducted from May 2001 to September 2003. Harvest areas included the Delaware Bay, Long Island Sound and Prince Edward Island. Occurrence followed a seasonal distribution. Low densities were observed in June, increased through August, and became rare by September. Given the ubiquitous nature of marine Vibrios, it was not surprising to find V. vulnificus everywhere, which was investigated. Observations confirmed the importance of strict time/temperature and product handling practices by shellfish harvesters, dealers and processors. Mishandling led to a potentially dangerous and uncharacteristically high V. vulnificus observation.

Big Bobby Bonaduce: Still Searching For The Rink Of Dreams, Don Morrow

Donald Morrow

No abstract provided.

"Open” Resources For The Agricultural Journalist, Joe Zumalt

Joseph R. Zumalt

Review of free resources available over the Web.

Stomping The Shadow: The Elevation Of Snowboarding To The Olympic Pedestal From A Jungian Perspective, M. Popovic, Don Morrow

Donald Morrow

No abstract provided.

Stage Independent Chloroquine Resistance And Chloroquine Toxicity Revealed Via Spinning Disk Confocal Microscopy, Bojana Gligorijevic, Kyle Purdy, David A. Elliott, Roland A. Cooper, Paul D. Roepe

Roland A. Cooper

Marine Recreational Fisheries Management Challenges And Mpas, Comparison Between The Mediterranean And Australia, Daryl Mcphee, Ana Gordoa, Xenia Illas

Daryl McPhee

No abstract provided.

Biological Impacts Of Recreational Fishing Resulting From Exploitation, Stocking And Introduction, Wolf-Christian Lewin, Daryl Mcphee, Robert Arlinghaus

Daryl McPhee

No abstract provided.

Nutrition: Concepts And Controversies, Frances Sizer, Ellie Whitney, Leonard Piché

Leonard Piché

No abstract provided.

New Connections Across Pathways And Cellular Processes: Industrialized Mutant Screening Reveals Novel Associations Between Diverse Phenotypes In Arabidopsis, Yan Lu, Linda Savage, Imad Ajjawi, Kathreen Imre, David Yoder, Christoph Benning, Dean Dellapenna, John Ohlrogge, Katherine Osteryoung, Andreas Weber, Curtis Wilkerson, Robert Last

Yan Lu

No abstract provided.

Mutant Rac1b Expression In Dictyostelium: Effects On Morphology, Growth, Endocytosis, Development, And The Actin Cytoskeleton, S. Palmieri, Thomas Nebl, Robert Pope, D. Seastone, E. Lee, E. Hinchcliffe, Greenfield Sluder, D. Knecht, J. Cardelli, Elizabeth Luna

Elizabeth J. Luna

Rac1 is a small G-protein in the Ras superfamily that has been implicated in the control of cell growth, adhesion, and the actin-based cytoskeleton. To investigate the role of Rac1 during motile processes, we have established Dictyostelium cell lines that conditionally overexpress epitope-tagged Dictyostelium discoideum wild-type Rac1B (DdRac1B) or a mutant DdRac1B protein. Expression of endogenous levels of myc- or GFP-tagged wild-type DdRac1B had minimal effect on cellular morphologies and behaviors. By contrast, expression of a constitutively active mutant (G12-->V or Q61-->L) or a dominant negative mutant (T17-->N) generated amoebae with characteristic cellular defects. The morphological appearance …

Patellin1, A Novel Sec14-Like Protein, Localizes To The Cell Plate And Binds Phosphoinositides, T. Peterman, Yamini Ohol, Lisa Mcreynolds, Elizabeth Luna

Elizabeth J. Luna

Membrane trafficking is central to construction of the cell plate during plant cytokinesis. Consequently, a detailed understanding of the process depends on the characterization of molecules that function in the formation, transport, targeting, and fusion of membrane vesicles to the developing plate, as well as those that participate in its consolidation and maturation into a fully functional partition. Here we report the initial biochemical and functional characterization of patellin1 (PATL1), a novel cell-plate-associated protein that is related in sequence to proteins involved in membrane trafficking in other eukaryotes. Analysis of the Arabidopsis genome indicated that PATL1 is one of a …

Identification By Peptide Analysis Of The Spectrin-Binding Protein In Human Erythrocytes, Elizabeth Luna, G. Kidd, D. Branton

Elizabeth J. Luna

One-dimensional and two-dimensional peptide-mapping techniques are used to identify the protein which gives rise to the 72,000 dalton alpha-chymotryptic fragment previously shown to be the membrane attachment site for spectrin. Peptide maps of the 72,000 dalton fragment are very different from maps of Bands 1, 2, 2.9, 3, 3.1, 4.1, and 4.2 and very similar to maps of the apparently closely homologous polypeptides, Bands 2.1, 2.2, 2.3, and 2.6. Limited proteolysis of erythrocyte membranes is shown to generate Band 3', another polypeptide which has been associated with spectrin-binding activity. Peptide maps of Band 3' are very similar to maps of …

Direct Binding Of F-Actin To Ponticulin, An Integral Plasma Membrane Glycoprotein, C. Chia, Anne Hitt, Elizabeth Luna

Elizabeth J. Luna

We have developed an 125I-labeled F-actin blot overlay assay for the identification of F-actin-binding proteins after transfer to nitrocellulose from SDS-polyacrylamide gels. Two major F-actin-binding proteins from Dictyostelium discoideum, a cytoplasmic 30 kDa protein and a 17 kDa integral membrane protein, and two minor membrane polypeptides of 19 kDa and 15 kDa were detected by this method. Using F-actin affinity and immunoaffinity chromatography, the 17 kDa polypeptide was identified as ponticulin, a previously described actin-binding glycoprotein from D. discoideum plasma membranes (Wuestehube, L.J., and Luna, E.J., [1987]: J. Cell Biol. 105:1741-1751). The binding of F-actin to ponticulin on blots is …

Merlin Differs From Moesin In Binding To F-Actin And In Its Intra- And Intermolecular Interactions, L. Huang, E. Ichimaru, Kersi Pestonjamasp, X. Cui, H. Nakamura, G. Lo, F. Lin, Elizabeth Luna, H. Furthmayr

Elizabeth J. Luna

The neurofibromatosis type 2 (NF2) tumor suppressor gene encodes merlin, a protein with homology to the cell membrane/F-actin linking proteins, moesin, ezrin and radixin. Unlike these closely related proteins, merlin lacks a C-terminal F-actin binding site detectable by actin blot overlays, and the GFP-tagged merlin C-terminal domain co-distributes with neither stress fibers nor cortical actin in NIH3T3 cells. Merlin also differs from the other three proteins in its inter- and intramolecular domain interactions, as shown by in vitro binding and yeast two-hybrid assays. As is true for ezrin, moesin and radixin, the N- and C-terminal domains of merlin type 1 …

A Stable, High Capacity, F-Actin Affinity Column, Elizabeth Luna, Y. Wang, E. Voss, D. Branton, D. Taylor

Elizabeth J. Luna

A high capacity F-actin affinity matrix is constructed by binding fluorescyl-actin to rabbit anti-fluorescein IgG that is covalently bound to Sepharose 4B. When stabilized with phalloidin, the actin remains associated with the Sepharose beads during repeated washes, activates the ATPase activity of myosin subfragment 1, and specifically binds 125I-heavy meromyosin and 125I-tropomyosin. The associations between the F-actin affinity matrix and the iodinated F-actin binding proteins are monitored both by affinity chromatography and by a rapid, low speed sedimentation assay. Anti-fluorescein IgG-Sepharose should be generally useful as a matrix for the immobilization of proteins containing accessible, covalently bound fluorescein groups.

F-Actin And Myosin Ii Binding Domains In Supervillin, Yu Chen, Norio Takizawa, Jessica Crowley, Sang Oh, Cheryl Gatto, Taketoshi Kambara, Osamu Sato, Xiang-Dong Li, Mitsuo Ikebe, Elizabeth Luna

Elizabeth J. Luna

Detergent-resistant membranes contain signaling and integral membrane proteins that organize cholesterol-rich domains called lipid rafts. A subset of these detergent-resistant membranes (DRM-H) exhibits a higher buoyant density ( approximately 1.16 g/ml) because of association with membrane skeleton proteins, including actin, myosin II, myosin 1G, fodrin, and an actin- and membrane-binding protein called supervillin (Nebl, T., Pestonjamasp, K. N., Leszyk, J. D., Crowley, J. L., Oh, S. W., and Luna, E. J. (2002) J. Biol. Chem. 277, 43399-43409). To characterize interactions among DRM-H cytoskeletal proteins, we investigated the binding partners of the novel supervillin N terminus, specifically amino acids 1-830. We …

Membrane Cytoskeleton: Pip(2) Pulls The Strings, Thomas Nebl, Sang Oh, Elizabeth Luna

Elizabeth J. Luna

A recent application of optical tweezers has shown that plasma membrane phosphatidylinositol 4,5-bisphosphate (PIP(2)) levels control adhesion of the membrane bilayer to the underlying cytoskeleton, by regulated direct binding of PIP(2) to cytoskeletal proteins and/or indirect effects on cytoskeleton structure.

Effects Of Cis-Ss-Ocimene, Cis-Sabinene Hydrate, And Monoterpene And Sesquiterpene Mixtures On Alfalfa Pellet Intake By Lambs, Ed Frederickson, R. Estell, Dean Anderson

Ed L. Frederickson

Interpretive Summary: The conversion of grasslands to shrublands in the western United States and in arid regions throughout the world is a serious concern to livestock producers and ecologists. This process of desertification not only reduces available forage for livestock and wildlife, but also leads to increased soil erosion and reduced biodiversity. These shrubs often contain chemicals that make them unpalatable to browsing animals. We have been conducting a long-term study on the impact of volatile plant chemicals such as terpenes on shrub consumption by livestock. Our previous work showed that for a common shrub in the northern Chihuahuan Desert …

Biomedical Research And The Law:--Embryonic Stem Cells, Clones And Genes: Science, Law, Politics, And Values, Michael J. Malinowski

Michael J. Malinowski

This article directly addresses the stem cell controversy, but also the broader history and norms regarding the roles of federal and state government in U.S. science research funding.

Wikipedia: A Multi-Lingual, Multi-Subject Resource Created By Potentially Anyone And Everyone, Joe Zumalt

Joseph R. Zumalt

Review of the rise of Wikipedia.

Fisheries Management In Australia, Daryl Mcphee

Daryl McPhee

Whether it’s throwing a fresh local prawn on the BBQ or dangling a line off the local jetty, fisheries resources are economically and socially important for many Australians. Australian fisheries have undergone significant management changes over the last decade and Australia is now recognised as a world leader.

This book is a comprehensive analysis of fisheries management in Australia. It provides practical insight into the cross-disciplinary tools of fisheries management. It takes the reader away from the outdated notion of “managing the fish” to the reality of managing human behaviour. It does so without losing track of the fundamental need …

Trapped In The Creation Museum, Stephen Asma

Stephen T Asma

Into the swampy debate over evolution has waded the new Creation Museum, in Petersburg, Ky. In an America divided between those who accept Darwin's theories and those who believe God created the world in six days, it seeks to win moderates and compromisers over to its side. Shortly after the museum opened last spring, I made a pilgrimage to witness this quirky new spectacle of Americana...

Gene Signature Of Stromal Cells Which Support Dendritic Cell Development., Geneviève Despars, Pravin Periasamy, Jonathan Tan, Janice Abbey, Terence O'Neill, Helen O'Neill

Helen O'Neill

Spleen stromal cells are critical determinants of dendritic cell (DC) development in spleen. The spleen stromal line, namely STX3, supports DC differentiation in vitro from overlaid bone marrow cells while the lymph node stromal line, namely 2RL22, does not. Here we have characterised the hematopoietic support capacity of each stroma, and analyzed lineage origin of the stromal cell lines by gene profiling using microarrays. Stromal co-culture experiments were performed using bone marrow cells as a source of hematopoietic progenitors. A characteristic immature myeloid-like CD11c+CD11b+CD86+MHC-II−/loB220−CD8α− DC is produced after 14 days in STX3 cocultures, while 2RL22 cocultures produce only monocyte/macrophage-like cells. …