Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Effects Of Phenobarbital On Serum And Liver Paraoxonase And Arylesterase Activities In Rats, Ahmet Ateşşahi̇n, İzzet Karahan, İbrahi̇m Pi̇ri̇nçci̇, M. Feri̇t Gürsu, Funda Gülcü
Effects Of Phenobarbital On Serum And Liver Paraoxonase And Arylesterase Activities In Rats, Ahmet Ateşşahi̇n, İzzet Karahan, İbrahi̇m Pi̇ri̇nçci̇, M. Feri̇t Gürsu, Funda Gülcü
Turkish Journal of Veterinary & Animal Sciences
This study was carried out to determine the effects of phenobarbital, administered orally and intraperitoneally over different periods, on paraoxonase, arylesterase, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) activities and total protein levels in the serum and liver. Thirty-six rats, 16-18 weeks old and weighing 240-260 g, were used. Phenobarbital was applied both orally and intraperitoneally at a dose of 100 mg/kg. Paraoxonase and arylesterase activities in serum and liver samples were measured spectrophotometrically using paraoxon and phenylasetate substrates, respectively. Although no significant changes were determined in serum paraoxonase and arylesterase activities, liver paraoxonase and arylesterase activities and total protein …
Morphometry Of The Lymph Nodes Excluding The Thoracic And Abdominal Cavity In Wistar Rats, İlknur Dabanoğlu, Hüsnü Erbay Bardakçioğlu, Fi̇gen Sevi̇l, Di̇dem Alp
Morphometry Of The Lymph Nodes Excluding The Thoracic And Abdominal Cavity In Wistar Rats, İlknur Dabanoğlu, Hüsnü Erbay Bardakçioğlu, Fi̇gen Sevi̇l, Di̇dem Alp
Turkish Journal of Veterinary & Animal Sciences
Ten male adult Wistar rats were used to obtain some measurements of the lymph nodes located in the head, neck, forelimb and hindlimb. The lymph nodes were marked by the injection of Evans Blue and were then dissected. In order to provide sufficient data for the morphometric properties of lymph nodes, the lymph nodes on each side were counted, and the longest and widest diameters of the lymph nodes were also measured. The mandibular lymph nodes were found to be higher in number in comparison with the other lymph nodes investigated. The largest was found in the axillary accessory lymph …
6-Phosphogluconate Dehydrogenase: Purification, Characterization And Kinetic Properties From Rat Erythrocytes, Şükrü Beydemi̇r, Mehmet Çi̇ftçi̇, Hayrullah Yilmaz, Ömer İrfan Küfrevi̇oğlu
6-Phosphogluconate Dehydrogenase: Purification, Characterization And Kinetic Properties From Rat Erythrocytes, Şükrü Beydemi̇r, Mehmet Çi̇ftçi̇, Hayrullah Yilmaz, Ömer İrfan Küfrevi̇oğlu
Turkish Journal of Veterinary & Animal Sciences
In this paper, a simple and rapid method for the purification of 6-phosphogluconate dehydrogenase from rat erythrocytes together with an analysis of the kinetic behavior and some properties of the enzyme are considered. The purification steps comprised high-speed centrifugation, 20-50% ammonium sulfate precipitation and 2', 5'-ADP Sepharose 4B affinity gel chromatography. The yield was 78.4% and the specific enzyme activity was 5.15 EU/mg proteins. The molecular mass of the subunit was estimated to be 59,566 Da by SDS polyacrylamide gel electrophoresis (SDS-PAGE) and native enzyme was found to be 111,000 Da by gel filtration column chromatography. The enzyme had an …