Open Access. Powered by Scholars. Published by Universities.®
- Discipline
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Highly Divergent Ssu Rrna Genes Found In The Marine Ciliates Myrionecta Rubra And Mesodinium Pulex, Torstein Tengs
Highly Divergent Ssu Rrna Genes Found In The Marine Ciliates Myrionecta Rubra And Mesodinium Pulex, Torstein Tengs
Dr. Torstein Tengs
Myrionecta rubra and Mesodinium pulex are among the most commonly encountered planktonic ciliates in coastal marine and estuarine regions throughout the world. Despite their widespread distribution, both ciliates have received little attention by taxonomists. In order to better understand the phylogenetic position of these ciliates, we determined the SSU rRNA gene sequence from cultures of M. rubra and M. pulex. Partial sequence data were also generated from isolated cells of M. rubra from Chesapeake Bay. The M. rubra and M. pulex sequences were very divergent from all other ciliates, but shared a branch with 100% bootstrap support. Both species had …
Genomic Representations Using Concatenates Of Type Iib Restriction Endonuclease Digestion Fragments, Torstein Tengs
Genomic Representations Using Concatenates Of Type Iib Restriction Endonuclease Digestion Fragments, Torstein Tengs
Dr. Torstein Tengs
We have developed a method for genomic representation using Type IIB restriction endonucleases. Representation by concatenation of restriction digests, or RECORD, is an approach to sample the fragments generated by cleavage with these enzymes. Here, we show that the RECORD libraries may be used for digital karyotyping and for pathogen identification by computational subtraction.
Development Of Real–Time Pcr Assays For The Detection Of Chattonella Species In Culture And Environmental Samples, Torstein Tengs
Development Of Real–Time Pcr Assays For The Detection Of Chattonella Species In Culture And Environmental Samples, Torstein Tengs
Dr. Torstein Tengs
Raphidophytes have been associated with fish kill events in Japanese, European and U.S. coastal waters, and many produce toxins that can pose a threat to human health. The recognition of raphidophytes as HAB species in mid-Atlantic estuarine waters led us to initiate molecular phylogenetic analyses of these taxa and to develop real-time PCR assays for rapid detection of important species. The molecular studies and PCR detection methods will enhance ongoing taxonomic, toxicologic and ecological of these organisms and will be a useful tool in HAB monitoring programs.