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Expression Of Luxi In Bacillus Thuringiensis For The Development Of A Bacteriophage-Based Bioreporter System For The Detection Of Anthrax, Amy Tomaszewski
Expression Of Luxi In Bacillus Thuringiensis For The Development Of A Bacteriophage-Based Bioreporter System For The Detection Of Anthrax, Amy Tomaszewski
Masters Theses
In light of the recent anthrax attacks of 2001in the United States, the need for a rapid, sensitive, and real-time surveillance system for detecting pathogenic bacteria has become readily apparent. A proof-of-principle study involving development of a bacteriophage-based bioreporter system for the detection of Bacillus anthracis spores has been initiated in the surrogate strain Bacillus thuringiensis 4Q7. The final goal of the project is to develop an engineered bacteriophage with the luxI gene from Vibrio fischeri, which upon infection of the target organism, would produce the signal molecule, N-(3-oxohexanoyl)-L-homoserine lactone (OHHL), capable of inducing bioluminescence in a neighboring …