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Role Of Ionic Interactions In The Catalytic Mechanism Of R67 Dihydrofolate Reductase, Stephanie Nicole Hicks
Role Of Ionic Interactions In The Catalytic Mechanism Of R67 Dihydrofolate Reductase, Stephanie Nicole Hicks
Masters Theses
The goal of this research is to investigate the role of K32 and K33 in the catalytic mechanism of R67 dihydrofolate reductase (DHFR). K32 is located in the active site pore and is the only charged residue in the active site while K33 is located on the surface of R67 DHFR. Both of the ligands for R67 DHFR, NADPH and dihydrofolate (DHF), have negative charges capable of forming ionic interactions with symmetry related K32 residues. NMR, DELPHI, and docking studies predict that K32 is involved in ionic interactions with the 2’phosphate of NADPH. Docking studies also predict that K32 participates …
Involvement Of Transit Peptide Aromatic Residues In Precursor Interaction(S) With The Chloroplast Import Apparatus, Huixia Zhang
Involvement Of Transit Peptide Aromatic Residues In Precursor Interaction(S) With The Chloroplast Import Apparatus, Huixia Zhang
Masters Theses
The import of nuclear-encoded preproteins into chloroplast is mediated by the transit peptide located in the N-terminus of the preproteins. A semi-conserved FGLK motif is identified in most of the transit peptides bioinformatically, despite the lack of homology in the primary sequence. To investigate the role of phenylalanines in the FGLK motifs of SStp in the interaction with outer envelope import apparatus, mutations were made on the two motifs found in prSSU from tobacco: F26TGLK and F35PVSRK. These mutations include F→W, F→S, and F→A in both of the motifs. The WT precursor (prSSU), 6 single prSSU mutants, 9 double prSSU …