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Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

2003

South Dakota State University

Plant Breeding and Genetics

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Full-Text Articles in Life Sciences

Gene Family Encoding Hordeum Vulgare Translation Elongation Factor 1 Alpha (Ef-1a): Assignment Based On Bioinformatic Analysis Of Cdna Sequences, Jackson Moeller Jan 2003

Gene Family Encoding Hordeum Vulgare Translation Elongation Factor 1 Alpha (Ef-1a): Assignment Based On Bioinformatic Analysis Of Cdna Sequences, Jackson Moeller

The Journal of Undergraduate Research

Search of international DNA database, Genbank, produced eight barley cDNA EFlα sequences with unique accession numbers. The length of these sequences varied from an incomplete 502 bp to a full length 1646 bp encoding a 447 amino acid protein. The knowledge that there are four EF-lα gene family members in barley led us to query whether all four genes were represented in the group of eight database submitted sequences. Also of great importance was the correct assignment of gene family member to its mRNA and protein expression profiles. Before we could begin to address the expression studies, we undertook the …