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Identification And Characterisation Of A Cdna Sequence Encoding A Glutamic Acid-Rich Protein Specifically Transcribed In Trichinella Spiralis Newborn Larvae And Recognised By Infected Swine Serum, Dante S. Zarlenga, Patricia Boyd, J. Ralph Lichtenfels, Dolores Hill, H. Ray Gamble
Identification And Characterisation Of A Cdna Sequence Encoding A Glutamic Acid-Rich Protein Specifically Transcribed In Trichinella Spiralis Newborn Larvae And Recognised By Infected Swine Serum, Dante S. Zarlenga, Patricia Boyd, J. Ralph Lichtenfels, Dolores Hill, H. Ray Gamble
Harold W. Manter Laboratory of Parasitology: Faculty and Staff Publications
Presently, little is known of the mechanism by which Trichinella penetrates and modulates reprogramming of muscle cells. In light of evidence demonstrating strong protective characteristics of antigens derived from this stage, understanding this process may shed light on potential targets for effective abatement of infection. To this end, a PCR-derived cDNA expression library was constructed using 0.5 mg of total RNA from Trichinella spiralis newborn larvae. The library consisted of >125,000 insert-containing clones. Approximately 40-50 × 103 clones were screened immunologically using sera from pigs experimentally infected with 7,000 Trichinella L1. Multiple clones reacting positively with the swine infection …