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Accumulation Of Poly-\Beta-Hydroxybutyrate In Streptomyces Species During Growth With Different Nitrogen Sources, Aysel Uğur, Nuretti̇n Şahi̇n, Yavuz Beyatli
Accumulation Of Poly-\Beta-Hydroxybutyrate In Streptomyces Species During Growth With Different Nitrogen Sources, Aysel Uğur, Nuretti̇n Şahi̇n, Yavuz Beyatli
Turkish Journal of Biology
The accumulation of poly-\beta-hydroxybutyric acid (PHB) in 27 Streptomyces isolates with different taxonomical properties was investigated. The amount of synthesized PHB was determined as crotonic acid by spectrophotometry. Eighty percent of the tested isolates accumulated PHB between 0.3 and 7.6% of dry mycelial weight. PHB was produced by a selectant of Streptomyces isolates in media containing different nitrogen sources (KNO_3, glycine, peptone, proteose peptone, L-asparagine, tryptone and malt extract), each combined with 2 g/l glucose as the carbon source. The most effective PHB production was observed on glycine/glucose medium with a 7.6% dry mycelial weight. Nitrogen limiting conditions were inhibitory …
Selective Isolation And Numerical Classification Of Novel Thermophilic Streptomycetes, Nevzat Şahi̇n, Erman Öztürk, Kami̇l Işik, Ergi̇n Kari̇ptaş, Reşi̇t Özkanca
Selective Isolation And Numerical Classification Of Novel Thermophilic Streptomycetes, Nevzat Şahi̇n, Erman Öztürk, Kami̇l Işik, Ergi̇n Kari̇ptaş, Reşi̇t Özkanca
Turkish Journal of Biology
Forty-five thermophilic streptomycetes were isolated from different habitats and examined for 163 unit characters with 8 representative thermophilic type strains, 29 reference strains and 16 randomly selected duplicated organisms for many phenotypic properties. The data were evaluated using the simple matching coefficients (S_{SM}), and clustering achieved using the complete algorithm. It was clearly seen that the high cophenetic correlation, low test error values and high similarity level agreed with the defined numerical taxonomic criteria. The test strains were assigned to four aggregate groups consisting seven major (5-8 strains), nine minor (2-4 strains) and ten single membered clusters. It can be …