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Increasing The Injured Area On Hypocotyl Explants Of Flax (Linum Usitatissimum L.) Leads To High Frequency Callus-Based Shoot Regeneration, Mustafa Yildiz, Celal Er
Increasing The Injured Area On Hypocotyl Explants Of Flax (Linum Usitatissimum L.) Leads To High Frequency Callus-Based Shoot Regeneration, Mustafa Yildiz, Celal Er
Turkish Journal of Biology
In this study, it was aimed to increase the frequency of shoots, which would be nominated to be transgenic after Agrobacterium inoculation, through expanding the wounding area by peeling the epidermis layer of hypocotyl explants of flax plants. Callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish were recorded as 0.8 g, 40%, 4.24 and 16.96, respectively in non-peeled explants. On the other hand, when the epidermis layer was peeled, callus weight, percentage of hypocotyls producing shoots, shoot number per explant and total shoot number per petri dish increased to 1.35 …
The Effect Of Different Explant Sources On Adventitious Shoot Regeneration In Flax (Linum Usitatissimum L.), Mustafa Yildiz, Sebahatti̇n Özcan, Celal Er
The Effect Of Different Explant Sources On Adventitious Shoot Regeneration In Flax (Linum Usitatissimum L.), Mustafa Yildiz, Sebahatti̇n Özcan, Celal Er
Turkish Journal of Biology
Hypocotyl and stem explants excised from in vitro and greenhouse-grown seedlings of Madaras, 1886 Sel. and Omega flax cultivars were compared in terms of percentage of explants producing shoots, shoot number per explant and total shoot number per petri dish. Hypocotyl explants resulted in better shoot regeneration. In vitro-grown seedlings were found to be more suitable than greenhouse-grown seedlings as an explant source.