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Protoplast Fusion In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Ekrem Gürel, Zeki̇ Kaya
Protoplast Fusion In Sugar Beet (Beta Vulgaris L.), Songül Gürel, Ekrem Gürel, Zeki̇ Kaya
Turkish Journal of Biology
In this study, attempts were made to fuse the suspension-derived protoplasts of a sugar beet breeding line (ÇBM315) with the mesophyll-derived protoplasts of three breeding lines (M114, ELK345 and M1017), which were obtained from our previous experiments. Combinations of three different PEG concentrations (20%, 25% or 30%) and three different treatment durations (15, 20 or 25 min) were tested. The fusion efficiency was determined by measuring the viability, heterokaryon frequency and plating efficiency (PE) of the fused protoplasts after four weeks of culture. In general, 25% PEG combined with 20 min treatment duration produced the highest fusion frequency, but the …