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Embryo Culture In Barley (Hordeum Vulgare L.), Betül Bürün, Esi̇n Çoban Poyrazoğlu
Embryo Culture In Barley (Hordeum Vulgare L.), Betül Bürün, Esi̇n Çoban Poyrazoğlu
Turkish Journal of Biology
The effects of medium and sterilization on the mature embryo culture of barley (Hordeum vulgare L.) were studied. Among the different media used, the highest plantlet development from embryos was obtained in Randolph and Cox medium (92.1%), followed by Murashige and Skoog (MS) (91.3%), half-strength MS (1/2 MS) (87.7%) and B5 (85.7%). Among the sterilization methods studied, sodium hypochlorite sterilization followed by treatment of antibiotic solution and sterilization HgCl_2 application was found to be more effective than the other methods. However, no statistically significant negative effect of HgCl_2 on the plantlet development from embryos was observed.