Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Purification And Determination Of The Molecular Structure Of Hemolymph Lectin Of Agrotis Segetum (Denis And Schiff.), Nursel Gül, Cevat Ayvali
Purification And Determination Of The Molecular Structure Of Hemolymph Lectin Of Agrotis Segetum (Denis And Schiff.), Nursel Gül, Cevat Ayvali
Turkish Journal of Biology
A soluble lectin was purified from the hemolymph of Agrotis segetum by two methods, via affinity chromatography on a Sepharose-4B column and gel filtration on a Superdex-200 column. Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) showed a single protein band with a molecular weight of 69,000 in both the presence and absence of 2-Mercaptoethanol. Alternatively, the molecular weight of this lectin was estimated to be 69,000 by gel filtration on Superdex-200. Electron microscopic observation of the purified lectin was reticular in structure and consisted of tandem aligned spherical basic units.